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Protein

Glycerol kinase

Gene

glpK

Organism
Xanthomonas campestris pv. vesicatoria (strain 85-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Key enzyme in the regulation of glycerol uptake and metabolism. Catalyzes the phosphorylation of glycerol to yield sn-glycerol 3-phosphate.UniRule annotation

Catalytic activityi

ATP + glycerol = ADP + sn-glycerol 3-phosphate.UniRule annotation

Enzyme regulationi

Inhibited by fructose 1,6-bisphosphate (FBP).UniRule annotation

Pathwayi: glycerol degradation via glycerol kinase pathway

This protein is involved in step 1 of the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol.UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. Glycerol kinase (glpK)
This subpathway is part of the pathway glycerol degradation via glycerol kinase pathway, which is itself part of Polyol metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes sn-glycerol 3-phosphate from glycerol, the pathway glycerol degradation via glycerol kinase pathway and in Polyol metabolism.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei13 – 131SubstrateUniRule annotation
Binding sitei17 – 171ATPUniRule annotation
Binding sitei135 – 1351SubstrateUniRule annotation
Binding sitei267 – 2671ATPUniRule annotation
Binding sitei310 – 3101ATP; via carbonyl oxygenUniRule annotation
Binding sitei314 – 3141ATP; via amide nitrogenUniRule annotation
Binding sitei329 – 3291ATPUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi13 – 153ATPUniRule annotation
Nucleotide bindingi411 – 4155ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Kinase, Transferase

Keywords - Biological processi

Glycerol metabolism

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciXCAM316273:GJF8-378-MONOMER.
UniPathwayiUPA00618; UER00672.

Names & Taxonomyi

Protein namesi
Recommended name:
Glycerol kinaseUniRule annotation (EC:2.7.1.30UniRule annotation)
Alternative name(s):
ATP:glycerol 3-phosphotransferaseUniRule annotation
GlycerokinaseUniRule annotation
Short name:
GKUniRule annotation
Gene namesi
Name:glpKUniRule annotation
Ordered Locus Names:XCV0372
OrganismiXanthomonas campestris pv. vesicatoria (strain 85-10)
Taxonomic identifieri316273 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
Proteomesi
  • UP000007069 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 499499Glycerol kinasePRO_1000020811Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316273.XCV0372.

Structurei

3D structure databases

ProteinModelPortaliQ3BYR0.
SMRiQ3BYR0. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni83 – 842Substrate bindingUniRule annotation
Regioni245 – 2462Substrate bindingUniRule annotation

Sequence similaritiesi

Belongs to the FGGY kinase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3BYR0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MEKQYVLAID QGTTSSRAML FDRQGKVAGV AQREFGQIFP QPGWVEHNPR
60 70 80 90 100
EIMTSVYTTI TELLNNAQID ARAIAGIGIT NQRETAVVWD KATGQPIYNA
110 120 130 140 150
IVWQSRQTKD ICTQLKEAGH EQMVREKTGL LIDAYFSGTK VKWILDHVEG
160 170 180 190 200
ARERAQKGEL AFGTIDSWLI WNLTGGKVHV TDYTNASRTM MFNIHTLEWD
210 220 230 240 250
AELLAMLDVP AQMLPDVRSS SEIYGMTQTQ YFYGEQVPIA GIAGDQQAAL
260 270 280 290 300
FGQACFEPGM AKNTYGTGCF MLMNTGDKAV ASKAGLLTTI AWGIDGKVEY
310 320 330 340 350
ALEGAIFVAG SVVQWLRDGL RMLGKASDSQ AYAERAGDND GVYIVPAFVG
360 370 380 390 400
LGAPYWRSDI RGAVFGLTRG TTKEHFVRAA VESMAYQTRD VLTAMQSDSG
410 420 430 440 450
IELKELRADG GAIANDFMAQ FQSDILNVPV LRPEVAETTA LGAAYLAGLA
460 470 480 490
TGFWGSREEI AKQWAVDRRF EPAMADDKRQ GLYAGWQQAV EATMGFRIS
Length:499
Mass (Da):54,818
Last modified:November 22, 2005 - v1
Checksum:iA91A6DFB99AEB1D2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039952 Genomic DNA. Translation: CAJ22003.1.
RefSeqiWP_011346063.1. NC_007508.1.

Genome annotation databases

EnsemblBacteriaiCAJ22003; CAJ22003; XCV0372.
KEGGixcv:XCV0372.
PATRICi24090094. VBIXanCam71633_0562.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039952 Genomic DNA. Translation: CAJ22003.1.
RefSeqiWP_011346063.1. NC_007508.1.

3D structure databases

ProteinModelPortaliQ3BYR0.
SMRiQ3BYR0. Positions 2-496.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316273.XCV0372.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ22003; CAJ22003; XCV0372.
KEGGixcv:XCV0372.
PATRICi24090094. VBIXanCam71633_0562.

Phylogenomic databases

eggNOGiENOG4108JQ0. Bacteria.
COG0554. LUCA.
HOGENOMiHOG000222134.
KOiK00864.
OMAiGWVEHEP.
OrthoDBiEOG6RZB46.

Enzyme and pathway databases

UniPathwayiUPA00618; UER00672.
BioCyciXCAM316273:GJF8-378-MONOMER.

Family and domain databases

HAMAPiMF_00186. Glycerol_kin.
InterProiIPR000577. Carb_kinase_FGGY.
IPR018485. Carb_kinase_FGGY_C.
IPR018483. Carb_kinase_FGGY_CS.
IPR018484. Carb_kinase_FGGY_N.
IPR005999. Glycerol_kin.
[Graphical view]
PfamiPF02782. FGGY_C. 1 hit.
PF00370. FGGY_N. 1 hit.
[Graphical view]
PIRSFiPIRSF000538. GlpK. 1 hit.
TIGRFAMsiTIGR01311. glycerol_kin. 1 hit.
PROSITEiPS00933. FGGY_KINASES_1. 1 hit.
PS00445. FGGY_KINASES_2. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 85-10.

Entry informationi

Entry nameiGLPK_XANC5
AccessioniPrimary (citable) accession number: Q3BYR0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: November 11, 2015
This is version 69 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.