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Protein

1,4-alpha-glucan branching enzyme GlgB 2

Gene

glgB2

Organism
Xanthomonas campestris pv. vesicatoria (strain 85-10)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position.UniRule annotation

Catalytic activityi

Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain.UniRule annotation

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei422 – 4221NucleophileUniRule annotation
Active sitei475 – 4751Proton donorUniRule annotation

GO - Molecular functioni

  1. 1,4-alpha-glucan branching enzyme activity Source: UniProtKB-HAMAP
  2. cation binding Source: InterPro
  3. hydrolase activity, hydrolyzing O-glycosyl compounds Source: InterPro

GO - Biological processi

  1. glycogen biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Carbohydrate metabolism, Glycogen biosynthesis, Glycogen metabolism

Enzyme and pathway databases

BioCyciXCAM316273:GJF8-460-MONOMER.
UniPathwayiUPA00164.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Names & Taxonomyi

Protein namesi
Recommended name:
1,4-alpha-glucan branching enzyme GlgB 2UniRule annotation (EC:2.4.1.18UniRule annotation)
Alternative name(s):
1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2UniRule annotation
Alpha-(1->4)-glucan branching enzyme 2UniRule annotation
Glycogen branching enzyme 2UniRule annotation
Short name:
BE 2UniRule annotation
Gene namesi
Name:glgB2UniRule annotation
Ordered Locus Names:XCV0452
OrganismiXanthomonas campestris pv. vesicatoria (strain 85-10)
Taxonomic identifieri316273 [NCBI]
Taxonomic lineageiBacteriaProteobacteriaGammaproteobacteriaXanthomonadalesXanthomonadaceaeXanthomonas
ProteomesiUP000007069: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 7437431,4-alpha-glucan branching enzyme GlgB 2PRO_0000260717Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi316273.XCV0452.

Structurei

3D structure databases

ProteinModelPortaliQ3BYI0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyl hydrolase 13 family. GlgB subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiWELFLPR.
OrthoDBiEOG6JX7GT.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3BYI0-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MSERQGGQEQ RTEADGMTTE GISETSQTLQ ALANGLPADA FAVLGPKPLA
60 70 80 90 100
EGRRQVRVLA PGAEAMGLID PRGKLLARMQ ASAIDGVFEG ILAADGPYRL
110 120 130 140 150
RIVWPDRVQE VEDPYAFAAT LDESLLLQIA AGDGQAVRRA LGAQHVHCGD
160 170 180 190 200
VPGVRFAVWA PHAQRVAVVG DFNGWDVRRH PMRQRIGGFW ELFLPRVEAG
210 220 230 240 250
ARYKYAVTAA DGRVLLKADP VARQTELPPA TASVVPSAAA FAWTDAAWMA
260 270 280 290 300
NRDPGAVPAP LSIYEVHAAS WRRDGHNQPL DWPTLAEQLI PYVQQLGFTH
310 320 330 340 350
IELLPITEHP FGGSWGYQPL GLYAPTARHG SPDGFAQFVD ACHRAGIGVI
360 370 380 390 400
LDWVSAHFPD DAHGLAQFDG AALYEHADPR EGMHRDWNTL IYNYGRPEVT
410 420 430 440 450
AYLLGSALEW IEHYHLDGLR VDAVASMLYR DYGRAEGEWV PNAHGGRENL
460 470 480 490 500
EAVAFLRQLN REIATQFPGV LTIAEESTAW PGVTAAISDG GLGFTHKWNM
510 520 530 540 550
GWMHDTLGYM QRDPAERAQH HSQLTFGLVY AFDERFVLPL SHDEVVHGTG
560 570 580 590 600
GLLGQMPGDD WRRFANLRAY LALMWAHPGD KLLFMGAEFG QWADWNHDQS
610 620 630 640 650
LDWHLLDGAR HRGMQQLVGD LNAALRRTPA LYRGSHRADG FDWSVADDAR
660 670 680 690 700
NSVLAFVRHD PAGGAPLLAV SNLTPQPHHD YHVGVPRAGL WREILNTDSA
710 720 730 740
HYGGSNLGNS GRLATEPVGM HGHAQRLRLT LPPLATIYLQ AEK
Length:743
Mass (Da):81,692
Last modified:November 22, 2005 - v1
Checksum:i993B9A7E05F84474
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039952 Genomic DNA. Translation: CAJ22083.1.
RefSeqiWP_011346122.1. NC_007508.1.
YP_362183.1. NC_007508.1.

Genome annotation databases

EnsemblBacteriaiCAJ22083; CAJ22083; XCV0452.
GeneIDi3732483.
KEGGixcv:XCV0452.
PATRICi24090254. VBIXanCam71633_0641.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AM039952 Genomic DNA. Translation: CAJ22083.1.
RefSeqiWP_011346122.1. NC_007508.1.
YP_362183.1. NC_007508.1.

3D structure databases

ProteinModelPortaliQ3BYI0.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316273.XCV0452.

Protein family/group databases

CAZyiCBM48. Carbohydrate-Binding Module Family 48.
GH13. Glycoside Hydrolase Family 13.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiCAJ22083; CAJ22083; XCV0452.
GeneIDi3732483.
KEGGixcv:XCV0452.
PATRICi24090254. VBIXanCam71633_0641.

Phylogenomic databases

eggNOGiCOG0296.
HOGENOMiHOG000283037.
KOiK00700.
OMAiWELFLPR.
OrthoDBiEOG6JX7GT.

Enzyme and pathway databases

UniPathwayiUPA00164.
BioCyciXCAM316273:GJF8-460-MONOMER.

Family and domain databases

Gene3Di2.60.40.10. 2 hits.
2.60.40.1180. 1 hit.
3.20.20.80. 1 hit.
HAMAPiMF_00685. GlgB.
InterProiIPR006048. A-amylase_b_C.
IPR006407. GlgB.
IPR015902. Glyco_hydro_13.
IPR013780. Glyco_hydro_13_b.
IPR006047. Glyco_hydro_13_cat_dom.
IPR004193. Glyco_hydro_13_N.
IPR013781. Glyco_hydro_catalytic_dom.
IPR017853. Glycoside_hydrolase_SF.
IPR013783. Ig-like_fold.
IPR014756. Ig_E-set.
[Graphical view]
PANTHERiPTHR10357. PTHR10357. 1 hit.
PfamiPF00128. Alpha-amylase. 1 hit.
PF02806. Alpha-amylase_C. 1 hit.
PF02922. CBM_48. 1 hit.
[Graphical view]
PIRSFiPIRSF000463. GlgB. 1 hit.
SUPFAMiSSF51445. SSF51445. 1 hit.
SSF81296. SSF81296. 1 hit.
TIGRFAMsiTIGR01515. branching_enzym. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: 85-10.

Entry informationi

Entry nameiGLGB2_XANC5
AccessioniPrimary (citable) accession number: Q3BYI0
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 28, 2006
Last sequence update: November 22, 2005
Last modified: January 7, 2015
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Glycosyl hydrolases
    Classification of glycosyl hydrolase families and list of entries
  2. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  3. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.