Reviewed, UniProtKB/Swiss-Prot Q3BYI0 (GLGB2_XANC5)

Names and origin

Protein names

Recommended name:
    1,4-alpha-glucan-branching enzyme 2
    EC=2.4.1.18
Alternative name(s):
    Glycogen-branching enzyme 2
      Short name=BE 2
    1,4-alpha-D-glucan:1,4-alpha-D-glucan 6-glucosyl-transferase 2

Gene names

Name:	glgB2
Ordered Locus Names:	XCV0452

Organism

Xanthomonas campestris pv. vesicatoria (strain 85-10) [Complete proteome] [HAMAP]

Taxonomic identifier

316273 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	743 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the formation of the alpha-1,6-glucosidic linkages in glycogen by scission of a 1,4-alpha-linked oligosaccharide from growing alpha-1,4-glucan chains and the subsequent attachment of the oligosaccharide to the alpha-1,6 position By similarity.
Catalytic activity	Transfers a segment of a (1->4)-alpha-D-glucan chain to a primary hydroxy group in a similar glucan chain. HAMAP MF_00685
Pathway	Glycan biosynthesis; glycogen biosynthesis. HAMAP MF_00685
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the glycosyl hydrolase 13 family.

Ontologies

Keywords
Biological process	Glycogen biosynthesis
Molecular function	Glycosyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycogen biosynthetic process Inferred from electronic annotation. Source: HAMAP
Molecular function	1,4-alpha-glucan branching enzyme activity Inferred from electronic annotation. Source: HAMAP cation binding Inferred from electronic annotation. Source: InterPro hydrolase activity, hydrolyzing O-glycosyl compounds Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 743	743	1,4-alpha-glucan-branching enzyme 2 HAMAP MF_00685		PRO_0000260717
Sites
	Active site	317	1	By similarity
	Active site	352	1	By similarity
	Active site	357	1	By similarity
	Active site	420	1	By similarity
	Active site	422	1	By similarity
	Active site	475	1	By similarity
	Active site	542	1	By similarity
	Active site	543	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3BYI0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 993B9A7E05F84474	FASTA	743	81,692
	10 20 30 40 50 60 MSERQGGQEQ RTEADGMTTE GISETSQTLQ ALANGLPADA FAVLGPKPLA EGRRQVRVLA 70 80 90 100 110 120 PGAEAMGLID PRGKLLARMQ ASAIDGVFEG ILAADGPYRL RIVWPDRVQE VEDPYAFAAT 130 140 150 160 170 180 LDESLLLQIA AGDGQAVRRA LGAQHVHCGD VPGVRFAVWA PHAQRVAVVG DFNGWDVRRH 190 200 210 220 230 240 PMRQRIGGFW ELFLPRVEAG ARYKYAVTAA DGRVLLKADP VARQTELPPA TASVVPSAAA 250 260 270 280 290 300 FAWTDAAWMA NRDPGAVPAP LSIYEVHAAS WRRDGHNQPL DWPTLAEQLI PYVQQLGFTH 310 320 330 340 350 360 IELLPITEHP FGGSWGYQPL GLYAPTARHG SPDGFAQFVD ACHRAGIGVI LDWVSAHFPD 370 380 390 400 410 420 DAHGLAQFDG AALYEHADPR EGMHRDWNTL IYNYGRPEVT AYLLGSALEW IEHYHLDGLR 430 440 450 460 470 480 VDAVASMLYR DYGRAEGEWV PNAHGGRENL EAVAFLRQLN REIATQFPGV LTIAEESTAW 490 500 510 520 530 540 PGVTAAISDG GLGFTHKWNM GWMHDTLGYM QRDPAERAQH HSQLTFGLVY AFDERFVLPL 550 560 570 580 590 600 SHDEVVHGTG GLLGQMPGDD WRRFANLRAY LALMWAHPGD KLLFMGAEFG QWADWNHDQS 610 620 630 640 650 660 LDWHLLDGAR HRGMQQLVGD LNAALRRTPA LYRGSHRADG FDWSVADDAR NSVLAFVRHD 670 680 690 700 710 720 PAGGAPLLAV SNLTPQPHHD YHVGVPRAGL WREILNTDSA HYGGSNLGNS GRLATEPVGM 730 740 HGHAQRLRLT LPPLATIYLQ AEK

References

[1]

"Insights into genome plasticity and pathogenicity of the plant pathogenic Bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence." Thieme F., Koebnik R., Bekel T., Berger C., Boch J., Buettner D., Caldana C., Gaigalat L., Goesmann A., Kay S., Kirchner O., Lanz C., Linke B., McHardy A.C., Meyer F., Mittenhuber G., Nies D.H., Niesbach-Kloesgen U. , Patschkowski T., Rueckert C., Rupp O., Schneiker S., Schuster S.C., Vorhoelter F.J., Weber E., Puehler A., Bonas U., Bartels D., Kaiser O. J. Bacteriol. 187:7254-7266(2005) [PubMed: 16237009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	AM039952 Genomic DNA. Translation: CAJ22083.1.
RefSeq	YP_362183.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3BYI0.
Protein family/group databases
CAZy	CBM48. Carbohydrate-Binding Module Family 48. GH13. Glycoside Hydrolase Family 13.
Genome annotation databases
GeneID	3732483.
GenomeReviews	Gene locus XCV0452 in contig AM039952_GR.
KEGG	xcv:XCV0452.
NMPDR	fig|316273.3.peg.671.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3BYI0.
OMA	GFDWSVA.
Enzyme and pathway databases
BioCyc	XCAM316273:XCV0452-MON.
Family and domain databases
HAMAP	MF_00685. [Tree]
InterPro	IPR006407. 1-4-A-glucan_branch_enz_core. IPR006048. A-amylase_b_C. IPR013780. Glyco_hydro_13_b. IPR006047. Glyco_hydro_13_cat. IPR004193. Glyco_hydro_13_N. IPR013781. Glyco_hydro_sg_catalytic. IPR013783. Ig-like_fold. [Graphical view]
Gene3D	G3DSA:2.60.40.1180. Glyco_hydro_13_b. 1 hit. G3DSA:3.20.20.80. Glyco_hydro_cat. 1 hit. G3DSA:2.60.40.10. Ig-like_fold. 1 hit.
Pfam	PF00128. Alpha-amylase. 1 hit. PF02806. Alpha-amylase_C. 1 hit. PF02922. CBM_48. 1 hit. [Graphical view]
TIGRFAMs	TIGR01515. branching_enzym. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLGB2_XANC5

Accession

Primary (citable) accession number: Q3BYI0

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 28, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 34 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)