Reviewed, UniProtKB/Swiss-Prot Q3BWA6 (DNAE2_XANC5)

Names and origin

Protein names

Recommended name:
    Error-prone DNA polymerase
    EC=2.7.7.7

Gene names

Name:	dnaE2
Ordered Locus Names:	XCV1226

Organism

Xanthomonas campestris pv. vesicatoria (strain 85-10) [Complete proteome] [HAMAP]

Taxonomic identifier

316273 [NCBI]

Taxonomic lineage

Bacteria › Proteobacteria › Gammaproteobacteria › Xanthomonadales › Xanthomonadaceae › Xanthomonas

Protein attributes

Sequence length	1082 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	DNA polymerase involved in damage-induced mutagenesis and translesion synthesis (TLS). It is not the major replicative DNA polymerase By similarity. HAMAP MF_01902
Catalytic activity	Deoxynucleoside triphosphate + DNA(n) = diphosphate + DNA(n+1). HAMAP MF_01902
Subcellular location	Cytoplasm Probable HAMAP MF_01902.
Sequence similarities	Belongs to the DNA polymerase type-C family. DnaE2 subfamily.

Ontologies

Keywords
Biological process	DNA damage DNA repair DNA replication
Cellular component	Cytoplasm
Molecular function	DNA-directed DNA polymerase Nucleotidyltransferase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA repair Inferred from electronic annotation. Source: UniProtKB-KW DNA replication Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	3'-5' exonuclease activity Inferred from electronic annotation. Source: InterPro DNA binding Inferred from electronic annotation. Source: InterPro DNA-directed DNA polymerase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1082	1082	Error-prone DNA polymerase HAMAP MF_01902		PRO_1000070599

Sequences

	Sequence		Length	Mass (Da)
	Q3BWA6-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: FC52F840E3FDC1D2	FASTA	1,082	120,423
	10 20 30 40 50 60 MSWDDAIDGV DRDTPGGRMP RAWNVAARLR AANDDISHAH VADGLPAYAE LHCLSDFSFL 70 80 90 100 110 120 RGASSAEQLF VRAQHCGYSA LAITDECSLA GIVRGLEASR ATGVRLIVGS EFALVDGTRF 130 140 150 160 170 180 VLLVENAHGY PQLCGLITTA RRAASKGAYR LGRAEVQAQF RDVAPGVFAL WLPGAQPQAE 190 200 210 220 230 240 QGAWLQQVFG ERAFLAVELH REQDDVARLQ VLQALAQQLG MTALASGDVH MAQRRERIVQ 250 260 270 280 290 300 DTLTAIRHTL PLAECGAHLF RNGERHLRTR RALGNIYPDG LLQATVELAQ RCTFDISKLQ 310 320 330 340 350 360 YTYPRELVPQ GHTPASYLRQ LTEAGMRERW PDGVPKKIRD DIENELALIA ELEYEAFFLT 370 380 390 400 410 420 VQDVVRFARS KDILCQGRGS SANSAVCYAL SITSVDPSKT RLLISRFLSK ARNEPPDIDV 430 440 450 460 470 480 DFEHERREEV LQYVYAKYGR ERAALAATVI CYRGKSAVRD VAKAFGLPPD QIALLANCYG 490 500 510 520 530 540 WGNGETPMEQ RIEEAGFDLA NPLINKILLV TDHLRDHPRH LSQHVGGFVI SDEPLSLLVP 550 560 570 580 590 600 VENAAMADRT IIQWDKDDLE TMKLLKVDCL ALGMLTCIRK TLDLVRGHRH RDYTLATLPD 610 620 630 640 650 660 EDAATYKMIQ RADTVGVFQI ESRAQMAMLP RLKPAEFYDL VIEVAIVRPG PIQGDMVHPY 670 680 690 700 710 720 LQRRKDKSLI NYPSEGVEQI LGPTLGIPLF QEQVMELVIH AGYDPDEADK LRRSMAAWRR 730 740 750 760 770 780 GGDMESHRLR IRALMEGKGY ASSFIDQIFE QIKGFGSYGF PQSHAASFAK LVYASCWLKR 790 800 810 820 830 840 HEPAAFACGL LNAQPMGFYS ASQIVQDARR GSPERERVEV LPVDVLHSDW DNTLVGGRPW 850 860 870 880 890 900 RSAADPGEQP AIRLGMRQVA GLSQVVAQRI VAARTQRAFA DIGDLCLRAA LDEKARLALA 910 920 930 940 950 960 EAGALHGLVG NRNAARWVMA GVEARRPLLP GSPEERPVAF EAPRAGEEIL ADYRSVGLSL 970 980 990 1000 1010 1020 RQHPMALLRP QMRQRRILGL RELQGRRHGS GVHVAGLVTQ RQRPATAKGT IFVTLEDEQG 1030 1040 1050 1060 1070 1080 MINVIVWSHL ALRRRRALLE SRLLAVRGRW ERVDGVEHLI AGDLYDLSNL LGDMQLPSRD FH

References

[1]

"Insights into genome plasticity and pathogenicity of the plant pathogenic Bacterium Xanthomonas campestris pv. vesicatoria revealed by the complete genome sequence." Thieme F., Koebnik R., Bekel T., Berger C., Boch J., Buettner D., Caldana C., Gaigalat L., Goesmann A., Kay S., Kirchner O., Lanz C., Linke B., McHardy A.C., Meyer F., Mittenhuber G., Nies D.H., Niesbach-Kloesgen U. , Patschkowski T., Rueckert C., Rupp O., Schneiker S., Schuster S.C., Vorhoelter F.J., Weber E., Puehler A., Bonas U., Bartels D., Kaiser O. J. Bacteriol. 187:7254-7266(2005) [PubMed: 16237009] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AM039952 Genomic DNA. Translation: CAJ22857.1.
RefSeq	YP_362957.1.
3D structure databases
SMR	Q3BWA6. Positions 46-920.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3BWA6.
Genome annotation databases
GeneID	3733746.
GenomeReviews	Gene locus XCV1226 in contig AM039952_GR.
KEGG	xcv:XCV1226.
NMPDR	fig|316273.3.peg.289.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0587.
HOGENOM	HBG734490.
OMA	MGFYSPD.
Family and domain databases
HAMAP	MF_01902. DNApol_error_prone. [Tree]
InterPro	IPR011708. DNA_pol3_alpha. IPR004365. NA_bd_OB_tRNA-helicase. IPR004013. PHP_C. IPR003141. Pol/His_phosphatase_N. IPR016195. Pol/histidinol_Pase-like. IPR004805. PolC_alpha. [Graphical view]
Pfam	PF07733. DNA_pol3_alpha. 1 hit. PF02811. PHP. 1 hit. PF01336. tRNA_anti. 1 hit. [Graphical view]
SMART	SM00481. POLIIIAc. 1 hit. [Graphical view]
TIGRFAMs	TIGR00594. polc. 1 hit.
ProtoNet	Search...

Entry information

Entry name

DNAE2_XANC5

Accession

Primary (citable) accession number: Q3BWA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	February 5, 2008
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 35 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)