Q3BCR8 (TPMT_PANTR) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Pan troglodytes (Chimpanzee)

Taxonomic identifier

9598 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Primates › Haplorrhini › Catarrhini › Hominidae › Pan

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation Phosphoprotein
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000220110
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	14	1	Phosphoserine By similarity
	Modified residue	58	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3BCR8 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: DA0E738BC5EC7076	FASTA	245	28,174
	10 20 30 40 50 60 MDGTRTSLDI EEYSDTEVQK NQVLTLEEWQ DKWVNGKTAF HQEQGHQLLK KHLDTFLKGK 70 80 90 100 110 120 SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIREFF TEQNLSYSEE PITEIPGTKV 130 140 150 160 170 180 FKSSSGNISL YCCSIFDLPR TNIGKFDMIW DRGALVAINP GDRKCYADTM LSLLGKKFQY 190 200 210 220 230 240 LLCVLSYDPT KHPGPPFYVP HAEIERLFGK ICNIRCLEKV DAFEERHKSW GIDCLFEKLY LLTEK

References

[1]	"Thiopurine S-methyltransferase pharmacogenetics: variant allele functional and comparative genomics." Salavaggione O.E., Wang L., Wiepert M., Yee V.C., Weinshilboum R.M. Pharmacogenet. Genomics 15:801-815(2005) [PubMed: 16220112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY819777 mRNA. Translation: AAX37639.1.
RefSeq	NP_001030596.1. NM_001035519.1.
UniGene	Ptr.4400.
3D structure databases
ProteinModelPortal	Q3BCR8.
SMR	Q3BCR8. Positions 17-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3BCR8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
Ensembl	ENSPTRT00000032822; ENSPTRP00000030322; ENSPTRG00000017756.
GeneID	471863.
KEGG	ptr:471863.
Organism-specific databases
CTD	7172.
Phylogenomic databases
eggNOG	prNOG05434.
GeneTree	ENSGT00390000016823.
HOVERGEN	HBG003037.
InParanoid	Q3BCR8.
OMA	QGDIFTL.
OrthoDB	EOG4C5CKF.
PhylomeDB	Q3BCR8.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
KO	K00569.
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_PANTR

Accession

Primary (citable) accession number: Q3BCR8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 22, 2005
Last modified:	December 14, 2011
This is version 48 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program