Q3BCQ8 (TPMT_RABIT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Thiopurine S-methyltransferase
EC=2.1.1.67

Alternative name(s):
Thiopurine methyltransferase

Gene names

Name:

TPMT

Organism

Oryctolagus cuniculus (Rabbit) [Complete proteome]

Taxonomic identifier

9986 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Euarchontoglires › Glires › Lagomorpha › Leporidae › Oryctolagus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Catalyzes the S-methylation of thiopurine drugs such as 6-mercaptopurine.
Catalytic activity	S-adenosyl-L-methionine + a thiopurine = S-adenosyl-L-homocysteine + a thiopurine S-methylether.
Subunit structure	Monomer By similarity.
Subcellular location	Cytoplasm.
Sequence similarities	Belongs to the methyltransferase superfamily. TPMT family.

Ontologies

Keywords
Cellular component	Cytoplasm
Ligand	S-adenosyl-L-methionine
Molecular function	Methyltransferase Transferase
PTM	Acetylation
Technical term	Complete proteome
Gene Ontology (GO)
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	thiopurine S-methyltransferase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Thiopurine S-methyltransferase		PRO_0000220112
Sites
	Binding site	33	1	S-adenosyl-L-methionine By similarity
	Binding site	69	1	S-adenosyl-L-methionine; via carbonyl oxygen By similarity
	Binding site	90	1	S-adenosyl-L-methionine By similarity
	Binding site	152	1	S-adenosyl-L-methionine By similarity
Amino acid modifications
	Modified residue	58	1	N6-acetyllysine By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3BCQ8 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: C3973A3E662AD044	FASTA	245	28,283
	10 20 30 40 50 60 MDDARTLLDI KEYPDTEVQK NRVLTLEEWQ DKWVSHKIGF HQEQGHKLLK KHLDAFLKGE 70 80 90 100 110 120 SGLRVFFPLC GKAVEMKWFA DRGHSVVGVE ISELGIREFF SEQNLSYSEE PITEVPGAKV 130 140 150 160 170 180 FKSSAGNISL YCCSIFDLPR VNIGKFDRIW DRGALVAVNP SDRKRYAAVM LSLLRKGFQY 190 200 210 220 230 240 LLAVLSYDPT KHAGPPFYVP DAEIKMLFDT KCKIHCLEKV DAFEERHKSW GIDYIFEKLY LLTEK

References

[1]	"Thiopurine S-methyltransferase pharmacogenetics: variant allele functional and comparative genomics." Salavaggione O.E., Wang L., Wiepert M., Yee V.C., Weinshilboum R.M. Pharmacogenet. Genomics 15:801-815(2005) [PubMed: 16220112] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	AY827084 mRNA. Translation: AAX37648.1.
RefSeq	NP_001076153.1. NM_001082684.1.
UniGene	Ocu.3344.
3D structure databases
ProteinModelPortal	Q3BCQ8.
SMR	Q3BCQ8. Positions 14-245.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3BCQ8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	100009411.
Organism-specific databases
CTD	7172.
Phylogenomic databases
eggNOG	maNOG10315.
GeneTree	ENSGT00390000016823.
HOVERGEN	HBG003037.
OrthoDB	EOG4C5CKF.
Family and domain databases
InterPro	IPR008854. Thiopurine_S-MeTrfase. IPR016822. Thiopurine_S-MeTrfase_sub. [Graphical view]
PANTHER	PTHR10259. PTHR10259. 1 hit.
Pfam	PF05724. TPMT. 1 hit. [Graphical view]
PIRSF	PIRSF023956. Thiopurine_S-methyltransferase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

TPMT_RABIT

Accession

Primary (citable) accession number: Q3BCQ8

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 20, 2005
Last sequence update:	November 22, 2005
Last modified:	November 16, 2011
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program