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Protein

Presenilins-associated rhomboid-like protein, mitochondrial

Gene

Parl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity).By similarity

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei275 – 2751NucleophileBy similarity
Active sitei333 – 3331By similarity

GO - Molecular functioni

  1. endopeptidase activity Source: ParkinsonsUK-UCL
  2. serine-type endopeptidase activity Source: InterPro

GO - Biological processi

  1. negative regulation of intrinsic apoptotic signaling pathway Source: ParkinsonsUK-UCL
  2. negative regulation of release of cytochrome c from mitochondria Source: ParkinsonsUK-UCL
  3. proteolysis Source: ParkinsonsUK-UCL
  4. regulation of reactive oxygen species metabolic process Source: ParkinsonsUK-UCL
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilins-associated rhomboid-like protein, mitochondrial (EC:3.4.21.105)
Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL
Cleaved into the following chain:
P-beta
Short name:
Pbeta
Gene namesi
Name:ParlBy similarity
Synonyms:PsarlImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
ProteomesiUP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306191. Parl.

Subcellular locationi

Peptide P-beta :
  1. Nucleus By similarity

  2. Note: Translocated into the nucleus by an unknown mechanism.By similarity

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini51 – 9545Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei96 – 11621HelicalSequence AnalysisAdd
BLAST
Topological domaini117 – 16549Mitochondrial intermembraneSequence AnalysisAdd
BLAST
Transmembranei166 – 18621HelicalSequence AnalysisAdd
BLAST
Topological domaini187 – 21428Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei215 – 23521HelicalSequence AnalysisAdd
BLAST
Topological domaini236 – 2427Mitochondrial intermembraneSequence Analysis
Transmembranei243 – 26321HelicalSequence AnalysisAdd
BLAST
Topological domaini264 – 2685Mitochondrial matrixSequence Analysis
Transmembranei269 – 28921HelicalSequence AnalysisAdd
BLAST
Topological domaini290 – 2934Mitochondrial intermembraneSequence Analysis
Transmembranei294 – 31421HelicalSequence AnalysisAdd
BLAST
Topological domaini315 – 33117Mitochondrial matrixSequence AnalysisAdd
BLAST
Transmembranei332 – 35221HelicalSequence AnalysisAdd
BLAST
Topological domaini353 – 37725Mitochondrial intermembraneSequence AnalysisAdd
BLAST

GO - Cellular componenti

  1. integral component of membrane Source: UniProtKB-KW
  2. mitochondrial inner membrane Source: ParkinsonsUK-UCL
  3. nucleus Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transit peptidei1 – 5050MitochondrionBy similarityAdd
BLAST
Chaini51 – 377327Presenilins-associated rhomboid-like protein, mitochondrialPRO_0000257987Add
BLAST
Peptidei51 – 7525P-betaBy similarityPRO_0000257988Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei63 – 631PhosphoserineBy similarity
Modified residuei68 – 681PhosphoserineBy similarity

Post-translational modificationi

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PaxDbiQ3B8P0.
PRIDEiQ3B8P0.

PTM databases

PhosphoSiteiQ3B8P0.

Expressioni

Gene expression databases

GenevestigatoriQ3B8P0.

Interactioni

Subunit structurei

Interacts with PSEN1 and PSEN2. Binds OPA1 (By similarity).By similarity

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Sequence Analysis

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG0705.
HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ3B8P0.
KOiK09650.
PhylomeDBiQ3B8P0.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3B8P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALYSWVQRG WRCGQTWAPL LGGGYRELSA TQARQLLGRR FNLLLQQKCG
60 70 80 90 100
FRKAPRKVEP RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPVRTLLKPF
110 120 130 140 150
FFTVGFTGCA FGSAAIWQYE SLKSRVQSYF DGIKADWLDS IRPQKEGNLR
160 170 180 190 200
KEINKWWNSL SDGQRTVTGI IAANALVFCL WRVPSLHRTM IRYFTSNPAS
210 220 230 240 250
KVLCSPMLLS TFSHFSLFHM AANMYVLWSF STSIVNILGQ EQFVAVYLSA
260 270 280 290 300
GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP
310 320 330 340 350
VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL
360 370
IWKNREPLVK IWHEIRTNGP KKGGGSK
Length:377
Mass (Da):42,126
Last modified:November 22, 2005 - v1
Checksum:i4E27E9216BC0548C
GO
Isoform 21 Publication (identifier: Q3B8P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-105: Missing.

Note: No experimental confirmation available.Curated

Show »
Length:364
Mass (Da):40,619
Checksum:i282E7DA066C64A04
GO

Alternative sequence

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Alternative sequencei93 – 10513Missing in isoform 2. 1 PublicationVSP_052185Add
BLAST

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088226 mRNA. Translation: AAH88226.1.
BC105907 mRNA. Translation: AAI05908.1.
RefSeqiNP_001030326.1. NM_001035249.1. [Q3B8P0-1]
UniGeneiRn.7053.

Genome annotation databases

GeneIDi287979.
KEGGirno:287979.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088226 mRNA. Translation: AAH88226.1.
BC105907 mRNA. Translation: AAI05908.1.
RefSeqiNP_001030326.1. NM_001035249.1. [Q3B8P0-1]
UniGeneiRn.7053.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

PTM databases

PhosphoSiteiQ3B8P0.

Proteomic databases

PaxDbiQ3B8P0.
PRIDEiQ3B8P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287979.
KEGGirno:287979.

Organism-specific databases

CTDi55486.
RGDi1306191. Parl.

Phylogenomic databases

eggNOGiCOG0705.
HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ3B8P0.
KOiK09650.
PhylomeDBiQ3B8P0.

Miscellaneous databases

NextBioi627335.
PROiQ3B8P0.

Gene expression databases

GenevestigatoriQ3B8P0.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
    Tissue: LiverImported and ThymusImported.

Entry informationi

Entry nameiPARL_RAT
AccessioniPrimary (citable) accession number: Q3B8P0
Secondary accession number(s): Q5I0L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 22, 2005
Last modified: April 29, 2015
This is version 79 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.