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Q3B8P0 (PARL_RAT) Reviewed, UniProtKB/Swiss-Prot

Last modified July 9, 2014. Version 73. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Alt products·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Presenilins-associated rhomboid-like protein, mitochondrial

EC=3.4.21.105
Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL

Cleaved into the following chain:

  1. P-beta
    Short name=Pbeta
Gene names
Name:Parl
Synonyms:Psarl
OrganismRattus norvegicus (Rat) [Reference proteome]
Taxonomic identifier10116 [NCBI]
Taxonomic lineageEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus

Protein attributes

Sequence length377 AA.
Sequence statusComplete.
Sequence processingThe displayed sequence is further processed into a mature form.
Protein existenceEvidence at transcript level

General annotation (Comments)

Function

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals By similarity. UniProtKB Q5XJY4

Catalytic activity

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains. UniProtKB Q9H300

Subunit structure

Interacts with PSEN1 and PSEN2. Binds OPA1 By similarity. UniProtKB Q9H300 UniProtKB Q5XJY4

Subcellular location

Mitochondrion inner membrane; Multi-pass membrane protein By similarity.

P-beta: Nucleus By similarity. Note: Translocated into the nucleus by an unknown mechanism By similarity.

Post-translational modification

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner By similarity. UniProtKB Q9H300

Sequence similarities

Belongs to the peptidase S54 family.

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]
Isoform 1 Ref.1 (identifier: Q3B8P0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.
Isoform 2 Ref.1 (identifier: Q3B8P0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     93-105: Missing.
Note: No experimental confirmation available.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Transit peptide1 – 5050Mitochondrion By similarity UniProtKB Q9H300
Chain51 – 377327Presenilins-associated rhomboid-like protein, mitochondrial UniProtKB Q9H300
PRO_0000257987
Peptide51 – 7525P-beta By similarity UniProtKB Q9H300
PRO_0000257988

Regions

Topological domain51 – 9545Mitochondrial matrix Potential
Transmembrane96 – 11621Helical; Potential
Topological domain117 – 16549Mitochondrial intermembrane Potential
Transmembrane166 – 18621Helical; Potential
Topological domain187 – 21428Mitochondrial matrix Potential
Transmembrane215 – 23521Helical; Potential
Topological domain236 – 2427Mitochondrial intermembrane Potential
Transmembrane243 – 26321Helical; Potential
Topological domain264 – 2685Mitochondrial matrix Potential
Transmembrane269 – 28921Helical; Potential
Topological domain290 – 2934Mitochondrial intermembrane Potential
Transmembrane294 – 31421Helical; Potential
Topological domain315 – 33117Mitochondrial matrix Potential
Transmembrane332 – 35221Helical; Potential
Topological domain353 – 37725Mitochondrial intermembrane Potential

Sites

Active site2751Nucleophile By similarity UniProtKB P53259
Active site3331 By similarity UniProtKB P53259

Amino acid modifications

Modified residue631Phosphoserine By similarity
Modified residue681Phosphoserine By similarity

Natural variations

Alternative sequence93 – 10513Missing in isoform 2. Ref.1
VSP_052185

Sequences

Sequence LengthMass (Da)Tools
Isoform 1 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 4E27E9216BC0548C

FASTA37742,126
        10         20         30         40         50         60 
MALYSWVQRG WRCGQTWAPL LGGGYRELSA TQARQLLGRR FNLLLQQKCG FRKAPRKVEP 

        70         80         90        100        110        120 
RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPVRTLLKPF FFTVGFTGCA FGSAAIWQYE 

       130        140        150        160        170        180 
SLKSRVQSYF DGIKADWLDS IRPQKEGNLR KEINKWWNSL SDGQRTVTGI IAANALVFCL 

       190        200        210        220        230        240 
WRVPSLHRTM IRYFTSNPAS KVLCSPMLLS TFSHFSLFHM AANMYVLWSF STSIVNILGQ 

       250        260        270        280        290        300 
EQFVAVYLSA GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP 

       310        320        330        340        350        360 
VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL IWKNREPLVK 

       370 
IWHEIRTNGP KKGGGSK 

« Hide

Isoform 2 [UniParc].

Checksum: 282E7DA066C64A04
Show »

FASTA36440,619

References

[1]"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
The MGC Project Team
Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2).
Tissue: Liver and Thymus.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
BC088226 mRNA. Translation: AAH88226.1.
BC105907 mRNA. Translation: AAI05908.1.
RefSeqNP_001030326.1. NM_001035249.1. [Q3B8P0-1]
UniGeneRn.7053.

3D structure databases

ProteinModelPortalQ3B8P0.
ModBaseSearch...
MobiDBSearch...

PTM databases

PhosphoSiteQ3B8P0.

Proteomic databases

PaxDbQ3B8P0.
PRIDEQ3B8P0.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

GeneID287979.
KEGGrno:287979.

Organism-specific databases

CTD55486.
RGD1306191. Parl.

Phylogenomic databases

eggNOGCOG0705.
HOGENOMHOG000230670.
HOVERGENHBG082107.
InParanoidQ3B8P0.
KOK09650.
PhylomeDBQ3B8P0.

Gene expression databases

GenevestigatorQ3B8P0.

Family and domain databases

Gene3D1.20.1540.10. 1 hit.
InterProIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERPTHR22936. PTHR22936. 1 hit.
PfamPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetSearch...

Other

NextBio627335.

Entry information

Entry namePARL_RAT
AccessionPrimary (citable) accession number: Q3B8P0
Secondary accession number(s): Q5I0L2
Entry history
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 22, 2005
Last modified: July 9, 2014
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Peptidase families

Classification of peptidase families and list of entries