Q3B8P0 (PARL_RAT) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Presenilins-associated rhomboid-like protein, mitochondrial
EC=3.4.21.105

Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL

Cleaved into the following chain:

1. P-beta
   Short name=Pbeta

Gene names

Name:	Parl
Synonyms:	Psarl

Organism

Rattus norvegicus (Rat) [Reference proteome]

Taxonomic identifier

10116 [NCBI]

Taxonomic lineage

cellular organisms › Eukaryota › Opisthokonta › Metazoa › Eumetazoa › Bilateria › Deuterostomia › Chordata › Craniata › Vertebrata › Gnathostomata › Teleostomi › Euteleostomi › Sarcopterygii › Tetrapoda › Amniota › Mammalia › Theria › Eutheria › Euarchontoglires › Glires › Rodentia › Sciurognathi › Muroidea › Muridae › Murinae › Rattus

Protein attributes

Sequence length	377 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals By similarity. UniProtKB Q5XJY4
Catalytic activity	Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains. UniProtKB Q9H300
Subunit structure	Interacts with PSEN1 and PSEN2. Binds OPA1 By similarity. UniProtKB Q9H300 UniProtKB Q5XJY4
Subcellular location	Mitochondrion inner membrane; Multi-pass membrane protein By similarity. P-beta: Nucleus By similarity. Note: Translocated into the nucleus by an unknown mechanism By similarity.
Post-translational modification	P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner By similarity. UniProtKB Q9H300
Sequence similarities	Belongs to the peptidase S54 family.

Ontologies

Keywords
Cellular component	Membrane Mitochondrion Mitochondrion inner membrane Nucleus
Coding sequence diversity	Alternative splicing
Domain	Transit peptide Transmembrane Transmembrane helix
Molecular function	Hydrolase Protease Serine protease
PTM	Phosphoprotein
Technical term	Complete proteome Reference proteome
Gene Ontology (GO)
Biological_process	proteolysis Inferred from electronic annotation. Source: UniProtKB-KW
Cellular_component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW mitochondrial inner membrane Inferred from electronic annotation. Source: UniProtKB-SubCell nucleus Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular_function	serine-type endopeptidase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative splicing. [Align] [Select]

Isoform 1 Ref.1 (identifier: Q3B8P0-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 Ref.1 (identifier: Q3B8P0-2)

The sequence of this isoform differs from the canonical sequence as follows:
     93-105: Missing.

Note: No experimental confirmation available.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – 50	50	Mitochondrion By similarity UniProtKB Q9H300
	Chain	51 – 377	327	Presenilins-associated rhomboid-like protein, mitochondrial UniProtKB Q9H300		PRO_0000257987
	Peptide	51 – 75	25	P-beta By similarity UniProtKB Q9H300		PRO_0000257988
Regions
	Topological domain	51 – 95	45	Mitochondrial matrix Potential
	Transmembrane	96 – 116	21	Helical; Potential
	Topological domain	117 – 165	49	Mitochondrial intermembrane Potential
	Transmembrane	166 – 186	21	Helical; Potential
	Topological domain	187 – 214	28	Mitochondrial matrix Potential
	Transmembrane	215 – 235	21	Helical; Potential
	Topological domain	236 – 242	7	Mitochondrial intermembrane Potential
	Transmembrane	243 – 263	21	Helical; Potential
	Topological domain	264 – 268	5	Mitochondrial matrix Potential
	Transmembrane	269 – 289	21	Helical; Potential
	Topological domain	290 – 293	4	Mitochondrial intermembrane Potential
	Transmembrane	294 – 314	21	Helical; Potential
	Topological domain	315 – 331	17	Mitochondrial matrix Potential
	Transmembrane	332 – 352	21	Helical; Potential
	Topological domain	353 – 377	25	Mitochondrial intermembrane Potential
Sites
	Active site	275	1	Nucleophile By similarity UniProtKB P53259
	Active site	333	1	By similarity UniProtKB P53259
Amino acid modifications
	Modified residue	63	1	Phosphoserine By similarity
	Modified residue	68	1	Phosphoserine By similarity
Natural variations
	Alternative sequence	93 – 105	13	Missing in isoform 2. Ref.1		VSP_052185

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 4E27E9216BC0548C	FASTA	377	42,126
	10 20 30 40 50 60 MALYSWVQRG WRCGQTWAPL LGGGYRELSA TQARQLLGRR FNLLLQQKCG FRKAPRKVEP 70 80 90 100 110 120 RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPVRTLLKPF FFTVGFTGCA FGSAAIWQYE 130 140 150 160 170 180 SLKSRVQSYF DGIKADWLDS IRPQKEGNLR KEINKWWNSL SDGQRTVTGI IAANALVFCL 190 200 210 220 230 240 WRVPSLHRTM IRYFTSNPAS KVLCSPMLLS TFSHFSLFHM AANMYVLWSF STSIVNILGQ 250 260 270 280 290 300 EQFVAVYLSA GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP 310 320 330 340 350 360 VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL IWKNREPLVK 370 IWHEIRTNGP KKGGGSK
	Isoform 2 [UniParc]. Checksum: 282E7DA066C64A04	FASTA	364	40,619
	10 20 30 40 50 60 MALYSWVQRG WRCGQTWAPL LGGGYRELSA TQARQLLGRR FNLLLQQKCG FRKAPRKVEP 70 80 90 100 110 120 RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPFTGCAFGS AAIWQYESLK SRVQSYFDGI 130 140 150 160 170 180 KADWLDSIRP QKEGNLRKEI NKWWNSLSDG QRTVTGIIAA NALVFCLWRV PSLHRTMIRY 190 200 210 220 230 240 FTSNPASKVL CSPMLLSTFS HFSLFHMAAN MYVLWSFSTS IVNILGQEQF VAVYLSAGVI 250 260 270 280 290 300 SNFVSYVCKV ATGRYGPSLG ASGAIMTVLA AVCTKIPEGR LAIIFLPVFT FTAGNALKAI 310 320 330 340 350 360 IAMDTAGMIL GWKFFDHAAH LGGALFGIWY ITYGHELIWK NREPLVKIWH EIRTNGPKKG GGSK

References

[1]	"The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)." The MGC Project Team Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Tissue: Liver and Thymus.
+	Additional computationally mapped references.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC088226 mRNA. Translation: AAH88226.1. BC105907 mRNA. Translation: AAI05908.1.
IPI	IPI00361872. IPI00781173.
RefSeq	NP_001030326.1. NM_001035249.1.
UniGene	Rn.7053.
3D structure databases
ProteinModelPortal	Q3B8P0.
ModBase	Search...
PTM databases
PhosphoSite	Q3B8P0.
Proteomic databases
PaxDb	Q3B8P0.
PRIDE	Q3B8P0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	287979.
KEGG	rno:287979.
Organism-specific databases
CTD	55486.
RGD	1306191. Parl.
Phylogenomic databases
eggNOG	COG0705.
HOGENOM	HOG000230670.
HOVERGEN	HBG082107.
InParanoid	Q3B8P0.
KO	K09650.
OrthoDB	EOG4ZGPCC.
Gene expression databases
ArrayExpress	Q3B8P0.
Genevestigator	Q3B8P0.
GermOnline	ENSRNOG00000023271. Rattus norvegicus.
Family and domain databases
InterPro	IPR002610. Peptidase_S54_rhomboid. IPR022764. Peptidase_S54_rhomboid_dom. [Graphical view]
PANTHER	PTHR22936. PTHR22936. 1 hit.
Pfam	PF01694. Rhomboid. 1 hit. [Graphical view]
ProtoNet	Search...
Other
NextBio	627335.

Entry information

Entry name

PARL_RAT

Accession

Primary (citable) accession number: Q3B8P0
Secondary accession number(s): Q5I0L2

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 22, 2005
Last modified:	April 3, 2013
This is version 62 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Chordata Protein Annotation Program