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Protein

Presenilins-associated rhomboid-like protein, mitochondrial

Gene

Parl

Organism
Rattus norvegicus (Rat)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Required for the control of apoptosis during postnatal growth. Essential for proteolytic processing of an antiapoptotic form of OPA1 which prevents the release of mitochondrial cytochrome c in response to intrinsic apoptoptic signals (By similarity).By similarity

Catalytic activityi

Cleaves type-1 transmembrane domains using a catalytic dyad composed of serine and histidine that are contributed by different transmembrane domains.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Active sitei275NucleophileBy similarity1
Active sitei333By similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase, Protease, Serine protease

Names & Taxonomyi

Protein namesi
Recommended name:
Presenilins-associated rhomboid-like protein, mitochondrial (EC:3.4.21.105)
Alternative name(s):
Mitochondrial intramembrane-cleaving protease PARL
Cleaved into the following chain:
P-beta
Short name:
Pbeta
Gene namesi
Name:ParlBy similarity
Synonyms:PsarlImported
OrganismiRattus norvegicus (Rat)
Taxonomic identifieri10116 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeRattus
Proteomesi
  • UP000002494 Componenti: Unplaced

Organism-specific databases

RGDi1306191. Parl.

Subcellular locationi

  • Mitochondrion inner membrane By similarity; Multi-pass membrane protein By similarity
P-beta :
  • Nucleus By similarity

  • Note: Translocated into the nucleus by an unknown mechanism.By similarity

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Topological domaini51 – 95Mitochondrial matrixSequence analysisAdd BLAST45
Transmembranei96 – 116HelicalSequence analysisAdd BLAST21
Topological domaini117 – 165Mitochondrial intermembraneSequence analysisAdd BLAST49
Transmembranei166 – 186HelicalSequence analysisAdd BLAST21
Topological domaini187 – 214Mitochondrial matrixSequence analysisAdd BLAST28
Transmembranei215 – 235HelicalSequence analysisAdd BLAST21
Topological domaini236 – 242Mitochondrial intermembraneSequence analysis7
Transmembranei243 – 263HelicalSequence analysisAdd BLAST21
Topological domaini264 – 268Mitochondrial matrixSequence analysis5
Transmembranei269 – 289HelicalSequence analysisAdd BLAST21
Topological domaini290 – 293Mitochondrial intermembraneSequence analysis4
Transmembranei294 – 314HelicalSequence analysisAdd BLAST21
Topological domaini315 – 331Mitochondrial matrixSequence analysisAdd BLAST17
Transmembranei332 – 352HelicalSequence analysisAdd BLAST21
Topological domaini353 – 377Mitochondrial intermembraneSequence analysisAdd BLAST25

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Membrane, Mitochondrion, Mitochondrion inner membrane, Nucleus

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transit peptidei1 – 50MitochondrionBy similarityAdd BLAST50
ChainiPRO_000025798751 – 377Presenilins-associated rhomboid-like protein, mitochondrialAdd BLAST327
PeptideiPRO_000025798851 – 75P-betaBy similarityAdd BLAST25

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei63PhosphoserineBy similarity1
Modified residuei68PhosphoserineBy similarity1

Post-translational modificationi

P-beta is proteolytically processed (beta-cleavage) in a PARL-dependent manner.By similarity

Keywords - PTMi

Phosphoprotein

Proteomic databases

PRIDEiQ3B8P0.

PTM databases

iPTMnetiQ3B8P0.
PhosphoSitePlusiQ3B8P0.

Interactioni

Subunit structurei

Interacts with PSEN1 and PSEN2. Binds OPA1 (By similarity).By similarity

Structurei

3D structure databases

ProteinModelPortaliQ3B8P0.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the peptidase S54 family.Sequence analysis

Keywords - Domaini

Transit peptide, Transmembrane, Transmembrane helix

Phylogenomic databases

HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ3B8P0.
KOiK09650.
PhylomeDBiQ3B8P0.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative splicing. AlignAdd to basket

Isoform 11 Publication (identifier: Q3B8P0-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MALYSWVQRG WRCGQTWAPL LGGGYRELSA TQARQLLGRR FNLLLQQKCG
60 70 80 90 100
FRKAPRKVEP RRSDTGSSGE AYKRSALIPP LEETVFYPSP YPVRTLLKPF
110 120 130 140 150
FFTVGFTGCA FGSAAIWQYE SLKSRVQSYF DGIKADWLDS IRPQKEGNLR
160 170 180 190 200
KEINKWWNSL SDGQRTVTGI IAANALVFCL WRVPSLHRTM IRYFTSNPAS
210 220 230 240 250
KVLCSPMLLS TFSHFSLFHM AANMYVLWSF STSIVNILGQ EQFVAVYLSA
260 270 280 290 300
GVISNFVSYV CKVATGRYGP SLGASGAIMT VLAAVCTKIP EGRLAIIFLP
310 320 330 340 350
VFTFTAGNAL KAIIAMDTAG MILGWKFFDH AAHLGGALFG IWYITYGHEL
360 370
IWKNREPLVK IWHEIRTNGP KKGGGSK
Length:377
Mass (Da):42,126
Last modified:November 22, 2005 - v1
Checksum:i4E27E9216BC0548C
GO
Isoform 21 Publication (identifier: Q3B8P0-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     93-105: Missing.

Note: No experimental confirmation available.Curated
Show »
Length:364
Mass (Da):40,619
Checksum:i282E7DA066C64A04
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_05218593 – 105Missing in isoform 2. 1 PublicationAdd BLAST13

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088226 mRNA. Translation: AAH88226.1.
BC105907 mRNA. Translation: AAI05908.1.
RefSeqiNP_001030326.1. NM_001035249.1. [Q3B8P0-1]
UniGeneiRn.7053.

Genome annotation databases

GeneIDi287979.
KEGGirno:287979.

Keywords - Coding sequence diversityi

Alternative splicing

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC088226 mRNA. Translation: AAH88226.1.
BC105907 mRNA. Translation: AAI05908.1.
RefSeqiNP_001030326.1. NM_001035249.1. [Q3B8P0-1]
UniGeneiRn.7053.

3D structure databases

ProteinModelPortaliQ3B8P0.
ModBaseiSearch...
MobiDBiSearch...

PTM databases

iPTMnetiQ3B8P0.
PhosphoSitePlusiQ3B8P0.

Proteomic databases

PRIDEiQ3B8P0.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

GeneIDi287979.
KEGGirno:287979.

Organism-specific databases

CTDi55486.
RGDi1306191. Parl.

Phylogenomic databases

HOGENOMiHOG000230670.
HOVERGENiHBG082107.
InParanoidiQ3B8P0.
KOiK09650.
PhylomeDBiQ3B8P0.

Miscellaneous databases

PROiQ3B8P0.

Family and domain databases

Gene3Di1.20.1540.10. 1 hit.
InterProiIPR002610. Peptidase_S54_rhomboid.
IPR022764. Peptidase_S54_rhomboid_dom.
[Graphical view]
PANTHERiPTHR22936. PTHR22936. 1 hit.
PfamiPF01694. Rhomboid. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiPARL_RAT
AccessioniPrimary (citable) accession number: Q3B8P0
Secondary accession number(s): Q5I0L2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: October 31, 2006
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 88 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Peptidase families
    Classification of peptidase families and list of entries
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.