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Q3B7Z2

- OSBP1_MOUSE

UniProt

Q3B7Z2 - OSBP1_MOUSE

Protein

Oxysterol-binding protein 1

Gene

Osbp

Organism
Mus musculus (Mouse)
Status
Reviewed - Annotation score: 3 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 80 (01 Oct 2014)
      Sequence version 3 (06 Feb 2013)
      Previous versions | rss
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    Functioni

    Binds cholesterol and a range of oxysterols. Cholesterol binding promotes the formation of a complex with PP2A and a tyrosine phosphatase which dephosphorylate ERK1/2, whereas 25-hydroxycholesterol causes its disassembly. Regulates cholesterol efflux by decreasing ABCA1 stability By similarity.By similarity

    GO - Molecular functioni

    1. lipid binding Source: UniProtKB-KW

    GO - Biological processi

    1. lipid transport Source: UniProtKB-KW

    Keywords - Biological processi

    Lipid transport, Transport

    Keywords - Ligandi

    Lipid-binding

    Enzyme and pathway databases

    ReactomeiREACT_198151. Sphingolipid de novo biosynthesis.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Oxysterol-binding protein 1
    Gene namesi
    Name:Osbp
    Synonyms:Kiaa4220
    OrganismiMus musculus (Mouse)
    Taxonomic identifieri10090 [NCBI]
    Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresGliresRodentiaSciurognathiMuroideaMuridaeMurinaeMusMus
    ProteomesiUP000000589: Chromosome 19

    Organism-specific databases

    MGIiMGI:97447. Osbp.

    Subcellular locationi

    Cytoplasm By similarity. Golgi apparatus membrane By similarity; Peripheral membrane protein By similarity
    Note: When bound to oxysterols, translocates to the periphery of Golgi membranes.By similarity

    GO - Cellular componenti

    1. Golgi membrane Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm, Golgi apparatus, Membrane

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 805805Oxysterol-binding protein 1PRO_0000349262Add
    BLAST

    Amino acid modifications

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Modified residuei188 – 1881Phosphoserine3 Publications
    Modified residuei191 – 1911Phosphoserine3 Publications
    Modified residuei196 – 1961Phosphoserine1 Publication
    Modified residuei238 – 2381Phosphoserine1 Publication
    Modified residuei349 – 3491Phosphoserine3 Publications
    Modified residuei380 – 3801Phosphoserine1 Publication
    Modified residuei387 – 3871PhosphoserineBy similarity

    Keywords - PTMi

    Phosphoprotein

    Proteomic databases

    MaxQBiQ3B7Z2.
    PaxDbiQ3B7Z2.
    PRIDEiQ3B7Z2.

    PTM databases

    PhosphoSiteiQ3B7Z2.

    Expressioni

    Gene expression databases

    GenevestigatoriQ3B7Z2.

    Interactioni

    Subunit structurei

    Homodimer or homotrimer. Interacts with VAPA By similarity.By similarity

    Structurei

    3D structure databases

    ProteinModelPortaliQ3B7Z2.
    SMRiQ3B7Z2. Positions 89-178, 344-378, 403-798.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Domains and Repeats

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Domaini86 – 17994PHPROSITE-ProRule annotationAdd
    BLAST

    Region

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Regioni404 – 45552Sterol bindingBy similarityAdd
    BLAST

    Coiled coil

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Coiled coili289 – 32436Sequence AnalysisAdd
    BLAST
    Coiled coili728 – 75932Sequence AnalysisAdd
    BLAST

    Domaini

    The PH domain control cholesterol binding without affecting 25-hydroxycholesterol binding.By similarity
    The second coiled-coil domain is required for interaction with the tyrosine phosphatase.By similarity

    Sequence similaritiesi

    Belongs to the OSBP family.Curated
    Contains 1 PH domain.PROSITE-ProRule annotation

    Keywords - Domaini

    Coiled coil

    Phylogenomic databases

    eggNOGiNOG281324.
    GeneTreeiENSGT00710000106456.
    HOGENOMiHOG000231233.
    HOVERGENiHBG053374.
    InParanoidiQ3B7Z2.
    OMAiEYWECKE.
    OrthoDBiEOG7MPRD7.
    TreeFamiTF320922.

    Family and domain databases

    Gene3Di2.30.29.30. 1 hit.
    InterProiIPR000648. Oxysterol-bd.
    IPR018494. Oxysterol-bd_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view]
    PANTHERiPTHR10972. PTHR10972. 1 hit.
    PfamiPF01237. Oxysterol_BP. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view]
    SMARTiSM00233. PH. 1 hit.
    [Graphical view]
    PROSITEiPS01013. OSBP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view]

    Sequences (2)i

    Sequence statusi: Complete.

    This entry describes 2 isoformsi produced by alternative splicing. Align

    Isoform 1 (identifier: Q3B7Z2-1) [UniParc]FASTAAdd to Basket

    This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

    « Hide

    MAATELRGVV GPGPAAIAAP GGGGAGPPAV GGGGGRGDAG PGPGVAAATA    50
    ATAGGPGPGA GGVAAGGPGS APPAAGSGGS GAGGSGSARE GWLFKWTNYI 100
    KGYQRRWFVL SNGLLSYYRS KAEMRHTCRG TINLATANIT VEDSCNFIIS 150
    NGGAQTYHLK ASSEVERQRW VTALELAKAK AVKMLAESDD SGDEESVSQT 200
    DKTELQSTLR TLSSKVEDLS TCNDLIAKHG TALQRSLSEL ESLKLPAESN 250
    EKIKQVNERA TLFRITSNAM INACRDFLML AQTHSKKWQK SLQYERDQRI 300
    RLEETLEQLA KQHNHLERAF RGATVLPANP PGSAGSGKDQ CCSGKGDMSD 350
    EDDENEFFDA PEIITMPENL GHKRTGSNIS GASSDVSLDE QYKHQLEETK 400
    KEKRTRIPYK PNYSLNLWSI MKNCIGKELS KIPMPVNFNE PLSMLQRLTE 450
    DLEYHELLDR AAKCENSLEQ LCYVAAFTVS SYSTTVFRTS KPFNPLLGET 500
    FELDRLEENG YRSICEQVSH HPPAAAHHAE SKNGWTLRQE IKITSKFRGK 550
    YLSIMPLGTI HCIFHSTGHH YTWKKVTTTV HNIIVGKLWI DQSGEIDIVN 600
    HKTGDKCNLK FVPYSYFSRD VARKVTGEVT DPSGKVHFAL LGTWDEKMDC 650
    FKVQAASGEN GGDARQRGHE AEDSRVMLWK RNPLPKNAEN MYYFSELALT 700
    LNAWEGGTAP TDSRLRPDQR LMENGRWDEA NAEKQRLEEK QRLSRKKREA 750
    EAAKATEDGT PHDPYKALWF ERKKDPVTRE LTHIYSGEYW ECKEKQDWGS 800
    CPDIF 805
    Length:805
    Mass (Da):88,797
    Last modified:February 6, 2013 - v3
    Checksum:i0922E5C6D660B425
    GO
    Isoform 2 (identifier: Q3B7Z2-2) [UniParc]FASTAAdd to Basket

    The sequence of this isoform differs from the canonical sequence as follows:
         1-268: Missing.
         269-272: AMIN → MLQL

    Note: No experimental confirmation available.

    Show »
    Length:537
    Mass (Da):61,670
    Checksum:i6FF692745FD305DC
    GO

    Sequence cautioni

    The sequence AAI07328.1 differs from that shown. Reason: Probable cloning artifact.
    The sequence AAI07329.1 differs from that shown. Reason: Probable cloning artifact.

    Alternative sequence

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Alternative sequencei1 – 268268Missing in isoform 2. 1 PublicationVSP_035281Add
    BLAST
    Alternative sequencei269 – 2724AMIN → MLQL in isoform 2. 1 PublicationVSP_035282

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK132663 mRNA. Translation: BAE21289.1.
    AC124464 Genomic DNA. No translation available.
    BC094342 mRNA. Translation: AAH94342.1.
    BC107327 mRNA. Translation: AAI07328.1. Sequence problems.
    BC107328 mRNA. Translation: AAI07329.1. Sequence problems.
    AK220301 mRNA. Translation: BAD90226.1.
    CCDSiCCDS37925.1. [Q3B7Z2-1]
    RefSeqiNP_001028346.1. NM_001033174.1. [Q3B7Z2-1]
    XP_006527502.1. XM_006527439.1. [Q3B7Z2-2]
    UniGeneiMm.291279.

    Genome annotation databases

    EnsembliENSMUST00000025590; ENSMUSP00000025590; ENSMUSG00000024687. [Q3B7Z2-1]
    GeneIDi76303.
    KEGGimmu:76303.
    UCSCiuc008gtk.1. mouse. [Q3B7Z2-2]

    Keywords - Coding sequence diversityi

    Alternative splicing

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    AK132663 mRNA. Translation: BAE21289.1 .
    AC124464 Genomic DNA. No translation available.
    BC094342 mRNA. Translation: AAH94342.1 .
    BC107327 mRNA. Translation: AAI07328.1 . Sequence problems.
    BC107328 mRNA. Translation: AAI07329.1 . Sequence problems.
    AK220301 mRNA. Translation: BAD90226.1 .
    CCDSi CCDS37925.1. [Q3B7Z2-1 ]
    RefSeqi NP_001028346.1. NM_001033174.1. [Q3B7Z2-1 ]
    XP_006527502.1. XM_006527439.1. [Q3B7Z2-2 ]
    UniGenei Mm.291279.

    3D structure databases

    ProteinModelPortali Q3B7Z2.
    SMRi Q3B7Z2. Positions 89-178, 344-378, 403-798.
    ModBasei Search...
    MobiDBi Search...

    PTM databases

    PhosphoSitei Q3B7Z2.

    Proteomic databases

    MaxQBi Q3B7Z2.
    PaxDbi Q3B7Z2.
    PRIDEi Q3B7Z2.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    Ensembli ENSMUST00000025590 ; ENSMUSP00000025590 ; ENSMUSG00000024687 . [Q3B7Z2-1 ]
    GeneIDi 76303.
    KEGGi mmu:76303.
    UCSCi uc008gtk.1. mouse. [Q3B7Z2-2 ]

    Organism-specific databases

    CTDi 5007.
    MGIi MGI:97447. Osbp.
    Rougei Search...

    Phylogenomic databases

    eggNOGi NOG281324.
    GeneTreei ENSGT00710000106456.
    HOGENOMi HOG000231233.
    HOVERGENi HBG053374.
    InParanoidi Q3B7Z2.
    OMAi EYWECKE.
    OrthoDBi EOG7MPRD7.
    TreeFami TF320922.

    Enzyme and pathway databases

    Reactomei REACT_198151. Sphingolipid de novo biosynthesis.

    Miscellaneous databases

    ChiTaRSi OSBP. mouse.
    NextBioi 344933.
    PROi Q3B7Z2.
    SOURCEi Search...

    Gene expression databases

    Genevestigatori Q3B7Z2.

    Family and domain databases

    Gene3Di 2.30.29.30. 1 hit.
    InterProi IPR000648. Oxysterol-bd.
    IPR018494. Oxysterol-bd_CS.
    IPR001849. PH_domain.
    IPR011993. PH_like_dom.
    [Graphical view ]
    PANTHERi PTHR10972. PTHR10972. 1 hit.
    Pfami PF01237. Oxysterol_BP. 1 hit.
    PF00169. PH. 1 hit.
    [Graphical view ]
    SMARTi SM00233. PH. 1 hit.
    [Graphical view ]
    PROSITEi PS01013. OSBP. 1 hit.
    PS50003. PH_DOMAIN. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. "The transcriptional landscape of the mammalian genome."
      Carninci P., Kasukawa T., Katayama S., Gough J., Frith M.C., Maeda N., Oyama R., Ravasi T., Lenhard B., Wells C., Kodzius R., Shimokawa K., Bajic V.B., Brenner S.E., Batalov S., Forrest A.R., Zavolan M., Davis M.J.
      , Wilming L.G., Aidinis V., Allen J.E., Ambesi-Impiombato A., Apweiler R., Aturaliya R.N., Bailey T.L., Bansal M., Baxter L., Beisel K.W., Bersano T., Bono H., Chalk A.M., Chiu K.P., Choudhary V., Christoffels A., Clutterbuck D.R., Crowe M.L., Dalla E., Dalrymple B.P., de Bono B., Della Gatta G., di Bernardo D., Down T., Engstrom P., Fagiolini M., Faulkner G., Fletcher C.F., Fukushima T., Furuno M., Futaki S., Gariboldi M., Georgii-Hemming P., Gingeras T.R., Gojobori T., Green R.E., Gustincich S., Harbers M., Hayashi Y., Hensch T.K., Hirokawa N., Hill D., Huminiecki L., Iacono M., Ikeo K., Iwama A., Ishikawa T., Jakt M., Kanapin A., Katoh M., Kawasawa Y., Kelso J., Kitamura H., Kitano H., Kollias G., Krishnan S.P., Kruger A., Kummerfeld S.K., Kurochkin I.V., Lareau L.F., Lazarevic D., Lipovich L., Liu J., Liuni S., McWilliam S., Madan Babu M., Madera M., Marchionni L., Matsuda H., Matsuzawa S., Miki H., Mignone F., Miyake S., Morris K., Mottagui-Tabar S., Mulder N., Nakano N., Nakauchi H., Ng P., Nilsson R., Nishiguchi S., Nishikawa S., Nori F., Ohara O., Okazaki Y., Orlando V., Pang K.C., Pavan W.J., Pavesi G., Pesole G., Petrovsky N., Piazza S., Reed J., Reid J.F., Ring B.Z., Ringwald M., Rost B., Ruan Y., Salzberg S.L., Sandelin A., Schneider C., Schoenbach C., Sekiguchi K., Semple C.A., Seno S., Sessa L., Sheng Y., Shibata Y., Shimada H., Shimada K., Silva D., Sinclair B., Sperling S., Stupka E., Sugiura K., Sultana R., Takenaka Y., Taki K., Tammoja K., Tan S.L., Tang S., Taylor M.S., Tegner J., Teichmann S.A., Ueda H.R., van Nimwegen E., Verardo R., Wei C.L., Yagi K., Yamanishi H., Zabarovsky E., Zhu S., Zimmer A., Hide W., Bult C., Grimmond S.M., Teasdale R.D., Liu E.T., Brusic V., Quackenbush J., Wahlestedt C., Mattick J.S., Hume D.A., Kai C., Sasaki D., Tomaru Y., Fukuda S., Kanamori-Katayama M., Suzuki M., Aoki J., Arakawa T., Iida J., Imamura K., Itoh M., Kato T., Kawaji H., Kawagashira N., Kawashima T., Kojima M., Kondo S., Konno H., Nakano K., Ninomiya N., Nishio T., Okada M., Plessy C., Shibata K., Shiraki T., Suzuki S., Tagami M., Waki K., Watahiki A., Okamura-Oho Y., Suzuki H., Kawai J., Hayashizaki Y.
      Science 309:1559-1563(2005) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORM 2).
      Strain: C57BL/6J.
      Tissue: Testis.
    2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: C57BL/6J.
    3. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
      The MGC Project Team
      Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 58-805.
      Strain: C57BL/6.
    4. "Prediction of the coding sequences of mouse homologues of KIAA gene. The complete nucleotide sequences of mouse KIAA-homologous cDNAs identified by screening of terminal sequences of cDNA clones randomly sampled from size-fractionated libraries."
      Okazaki N., Kikuno R.F., Ohara R., Inamoto S., Nagase T., Ohara O., Koga H.
      Submitted (FEB-2005) to the EMBL/GenBank/DDBJ databases
      Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] OF 290-805.
      Tissue: Pancreatic islet.
    5. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188; SER-191; SER-196; SER-349 AND SER-380, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    6. "Specific phosphopeptide enrichment with immobilized titanium ion affinity chromatography adsorbent for phosphoproteome analysis."
      Zhou H., Ye M., Dong J., Han G., Jiang X., Wu R., Zou H.
      J. Proteome Res. 7:3957-3967(2008) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188; SER-191 AND SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Liver.
    7. "The phagosomal proteome in interferon-gamma-activated macrophages."
      Trost M., English L., Lemieux S., Courcelles M., Desjardins M., Thibault P.
      Immunity 30:143-154(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-188; SER-191; SER-238 AND SER-349, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    8. "Large scale localization of protein phosphorylation by use of electron capture dissociation mass spectrometry."
      Sweet S.M., Bailey C.M., Cunningham D.L., Heath J.K., Cooper H.J.
      Mol. Cell. Proteomics 8:904-912(2009) [PubMed] [Europe PMC] [Abstract]
      Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
      Tissue: Embryonic fibroblast.

    Entry informationi

    Entry nameiOSBP1_MOUSE
    AccessioniPrimary (citable) accession number: Q3B7Z2
    Secondary accession number(s): E9QPD4
    , Q3V163, Q52KH7, Q570Y8
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: September 2, 2008
    Last sequence update: February 6, 2013
    Last modified: October 1, 2014
    This is version 80 of the entry and version 3 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programChordata Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. MGD cross-references
      Mouse Genome Database (MGD) cross-references in UniProtKB/Swiss-Prot
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3