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Protein

Hydroxyacylglutathione hydrolase, mitochondrial

Gene

HAGH

Organism
Bos taurus (Bovine)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at transcript leveli

Functioni

Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid.By similarity

Catalytic activityi

S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.

Cofactori

Zn2+By similarityNote: Binds 2 Zn2+ ions per subunit.By similarity

Pathwayi: methylglyoxal degradation

This protein is involved in step 2 of the subpathway that synthesizes (R)-lactate from methylglyoxal.
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. Lactoylglutathione lyase (GLO1)
  2. Hydroxyacylglutathione hydrolase, mitochondrial (HAGH)
This subpathway is part of the pathway methylglyoxal degradation, which is itself part of Secondary metabolite metabolism.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-lactate from methylglyoxal, the pathway methylglyoxal degradation and in Secondary metabolite metabolism.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi102Zinc 1; via tele nitrogenBy similarity1
Metal bindingi104Zinc 1; via pros nitrogenBy similarity1
Metal bindingi106Zinc 2By similarity1
Metal bindingi107Zinc 2; via tele nitrogenBy similarity1
Metal bindingi158Zinc 1; via tele nitrogenBy similarity1
Metal bindingi182Zinc 1By similarity1
Metal bindingi182Zinc 2By similarity1
Metal bindingi221Zinc 2; via tele nitrogenBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

ReactomeiR-BTA-70268. Pyruvate metabolism.
UniPathwayiUPA00619; UER00676.

Names & Taxonomyi

Protein namesi
Recommended name:
Hydroxyacylglutathione hydrolase, mitochondrial (EC:3.1.2.6)
Alternative name(s):
Glyoxalase II
Short name:
Glx II
Gene namesi
Name:HAGH
OrganismiBos taurus (Bovine)
Taxonomic identifieri9913 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaLaurasiatheriaCetartiodactylaRuminantiaPecoraBovidaeBovinaeBos
Proteomesi
  • UP000009136 Componenti: Chromosome 25

Subcellular locationi

Isoform 1 :
  • Mitochondrion matrix By similarity
Isoform 2 :
  • Cytoplasm By similarity

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm, Mitochondrion

Pathology & Biotechi

Chemistry databases

ChEMBLiCHEMBL3627591.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_0000309592? – 308Hydroxyacylglutathione hydrolase, mitochondrial
Transit peptidei1 – ?MitochondrionSequence analysis

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei116N6-acetyllysineBy similarity1
Modified residuei229N6-acetyllysine; alternateBy similarity1
Modified residuei229N6-succinyllysine; alternateBy similarity1

Keywords - PTMi

Acetylation

Proteomic databases

PaxDbiQ3B7M2.
PeptideAtlasiQ3B7M2.
PRIDEiQ3B7M2.

Expressioni

Gene expression databases

BgeeiENSBTAG00000019998.
ExpressionAtlasiQ3B7M2. differential.

Interactioni

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026635.

Structurei

3D structure databases

ProteinModelPortaliQ3B7M2.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni191 – 193Substrate bindingBy similarity3
Regioni221 – 223Substrate bindingBy similarity3
Regioni297 – 300Substrate bindingBy similarity4

Sequence similaritiesi

Keywords - Domaini

Transit peptide

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
HOVERGENiHBG001152.
InParanoidiQ3B7M2.
KOiK01069.
OMAiHVKCLST.
OrthoDBiEOG091G0E58.
TreeFamiTF105273.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.

Sequences (2)i

Sequence statusi: Complete.

Sequence processingi: The displayed sequence is further processed into a mature form.

This entry describes 2 isoformsi produced by alternative initiation. AlignAdd to basket

Isoform 1 (identifier: Q3B7M2-1) [UniParc]FASTAAdd to basket

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

« Hide

        10         20         30         40         50
MVLGRGLLGR WSVAELGAVC ARLGLGPALL GSLHHLGLRK SLTVDQGTMK
60 70 80 90 100
VELLPALTDN YMYLLIDEDT KEAAIVDPVQ PQKVVETARK HGVKLTTVLT
110 120 130 140 150
THHHWDHAGG NEKLVKLEPG LKVYGGDDRI GALTHKVTHL STLQVGSLHV
160 170 180 190 200
KCLSTPCHTS GHICYFVTKP NSPEPPAVFT GDTLFVAGCG KFYEGTADEM
210 220 230 240 250
YKALLEVLGR LPADTRVYCG HEYTINNLKF ARHVEPDNTA VREKLAWAKE
260 270 280 290 300
KYSIGEPTVP STIAEEFTYN PFMRVREKTV QQHAGETEPV ATMRAIRKEK

DQFKMPRD
Length:308
Mass (Da):34,089
Last modified:September 1, 2009 - v3
Checksum:iB67A0140178AF9C5
GO
Isoform 2 (identifier: Q3B7M2-2) [UniParc]FASTAAdd to basket

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Note: Produced by alternative initiation at Met-49 of isoform 1. Alternative initiation has been proven in human.
Show »
Length:260
Mass (Da):29,156
Checksum:i1D7F3F31EB89279B
GO

Alternative sequence

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Alternative sequenceiVSP_0379271 – 48Missing in isoform 2. 1 PublicationAdd BLAST48

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107545 mRNA. Translation: AAI07546.1.
RefSeqiNP_001030351.1. NM_001035274.1. [Q3B7M2-1]
UniGeneiBt.20141.

Genome annotation databases

EnsembliENSBTAT00000026635; ENSBTAP00000026635; ENSBTAG00000019998. [Q3B7M2-1]
GeneIDi509274.
KEGGibta:509274.

Keywords - Coding sequence diversityi

Alternative initiation

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
BC107545 mRNA. Translation: AAI07546.1.
RefSeqiNP_001030351.1. NM_001035274.1. [Q3B7M2-1]
UniGeneiBt.20141.

3D structure databases

ProteinModelPortaliQ3B7M2.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi9913.ENSBTAP00000026635.

Chemistry databases

ChEMBLiCHEMBL3627591.

Proteomic databases

PaxDbiQ3B7M2.
PeptideAtlasiQ3B7M2.
PRIDEiQ3B7M2.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENSBTAT00000026635; ENSBTAP00000026635; ENSBTAG00000019998. [Q3B7M2-1]
GeneIDi509274.
KEGGibta:509274.

Organism-specific databases

CTDi3029.

Phylogenomic databases

eggNOGiKOG0813. Eukaryota.
COG0491. LUCA.
GeneTreeiENSGT00530000063033.
HOGENOMiHOG000058041.
HOVERGENiHBG001152.
InParanoidiQ3B7M2.
KOiK01069.
OMAiHVKCLST.
OrthoDBiEOG091G0E58.
TreeFamiTF105273.

Enzyme and pathway databases

UniPathwayiUPA00619; UER00676.
ReactomeiR-BTA-70268. Pyruvate metabolism.

Gene expression databases

BgeeiENSBTAG00000019998.
ExpressionAtlasiQ3B7M2. differential.

Family and domain databases

Gene3Di3.60.15.10. 1 hit.
HAMAPiMF_01374. Glyoxalase_2. 1 hit.
InterProiIPR032282. HAGH_C.
IPR017782. Hydroxyacylglutathione_Hdrlase.
IPR001279. Metallo-B-lactamas.
[Graphical view]
PfamiPF16123. HAGH_C. 1 hit.
PF00753. Lactamase_B. 1 hit.
[Graphical view]
PIRSFiPIRSF005457. Glx. 1 hit.
SMARTiSM00849. Lactamase_B. 1 hit.
[Graphical view]
SUPFAMiSSF56281. SSF56281. 1 hit.
TIGRFAMsiTIGR03413. GSH_gloB. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiGLO2_BOVIN
AccessioniPrimary (citable) accession number: Q3B7M2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 13, 2007
Last sequence update: September 1, 2009
Last modified: November 30, 2016
This is version 80 of the entry and version 3 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program

Miscellaneousi

Caution

Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.Curated

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.