Reviewed, UniProtKB/Swiss-Prot Q3B7M2 (GLO2_BOVIN)

Names and origin

Protein names

Recommended name:
    Hydroxyacylglutathione hydrolase, mitochondrial
    EC=3.1.2.6
Alternative name(s):
    Glyoxalase II
      Short name=Glx II

Gene names

Name:

HAGH

Organism

Bos taurus (Bovine)

Taxonomic identifier

9913 [NCBI]

Taxonomic lineage

Eukaryota › Metazoa › Chordata › Craniata › Vertebrata › Euteleostomi › Mammalia › Eutheria › Laurasiatheria › Cetartiodactyla › Ruminantia › Pecora › Bovidae › Bovinae › Bos

Protein attributes

Sequence length	308 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is further processed into a mature form.
Protein existence	Evidence at transcript level.

General annotation (Comments)

Function	Thiolesterase that catalyzes the hydrolysis of S-D-lactoyl-glutathione to form glutathione and D-lactic acid By similarity.
Catalytic activity	S-(2-hydroxyacyl)glutathione + H2O = glutathione + a 2-hydroxy carboxylate.
Cofactor	Binds 2 zinc ions per subunit By similarity.
Pathway	Secondary metabolite metabolism; methylglyoxal degradation; (R)-lactate from methylglyoxal: step 2/2.
Subcellular location	Isoform 1: Mitochondrion matrix By similarity. Isoform 2: Cytoplasm By similarity.
Sequence similarities	Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family.
Caution	Only one single gene encoding glyoxalase II has been identified in vertebrates. In yeast and higher plants, separate genes encode the cytosolic and mitochondrial forms of glyoxalase II.

Ontologies

Keywords
Cellular component	Cytoplasm Mitochondrion
Coding sequence diversity	Alternative initiation
Domain	Transit peptide
Ligand	Metal-binding Zinc
Molecular function	Hydrolase
PTM	Acetylation
Gene Ontology (GO)
Cellular component	mitochondrial matrix Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	hydroxyacylglutathione hydrolase activity Inferred from electronic annotation. Source: EC zinc ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Alternative products

This entry describes 2 isoforms produced by alternative initiation. [Align] [Select]

Isoform 1 (identifier: Q3B7M2-1)

This isoform has been chosen as the 'canonical' sequence. All positional information in this entry refers to it. This is also the sequence that appears in the downloadable versions of the entry.

Isoform 2 (identifier: Q3B7M2-2)

The sequence of this isoform differs from the canonical sequence as follows:
     1-48: Missing.

Note: Produced by alternative initiation at Met-49 of isoform 1. Alternative initiation has been proven in human.

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Transit peptide	1 – ?		Mitochondrion Potential
	Chain	? – 308		Hydroxyacylglutathione hydrolase, mitochondrial		PRO_0000309592
Regions
	Region	191 – 193	3	Substrate By similarity
	Region	221 – 223	3	Substrate By similarity
	Region	297 – 300	4	Substrate By similarity
Sites
	Metal binding	102	1	Zinc 1 By similarity
	Metal binding	104	1	Zinc 1 By similarity
	Metal binding	106	1	Zinc 2 By similarity
	Metal binding	107	1	Zinc 2 By similarity
	Metal binding	158	1	Zinc 1 By similarity
	Metal binding	182	1	Zinc 1 By similarity
	Metal binding	182	1	Zinc 2 By similarity
	Metal binding	221	1	Zinc 2 By similarity
Amino acid modifications
	Modified residue	229	1	N6-acetyllysine By similarity
Natural variations
	Alternative sequence	1 – 48	48	Missing in isoform 2.		VSP_037927

Sequences

	Sequence		Length	Mass (Da)
	Isoform 1 [UniParc]. Last modified September 1, 2009. Version 3. Checksum: B67A0140178AF9C5	FASTA	308	34,089
	10 20 30 40 50 60 MVLGRGLLGR WSVAELGAVC ARLGLGPALL GSLHHLGLRK SLTVDQGTMK VELLPALTDN 70 80 90 100 110 120 YMYLLIDEDT KEAAIVDPVQ PQKVVETARK HGVKLTTVLT THHHWDHAGG NEKLVKLEPG 130 140 150 160 170 180 LKVYGGDDRI GALTHKVTHL STLQVGSLHV KCLSTPCHTS GHICYFVTKP NSPEPPAVFT 190 200 210 220 230 240 GDTLFVAGCG KFYEGTADEM YKALLEVLGR LPADTRVYCG HEYTINNLKF ARHVEPDNTA 250 260 270 280 290 300 VREKLAWAKE KYSIGEPTVP STIAEEFTYN PFMRVREKTV QQHAGETEPV ATMRAIRKEK DQFKMPRD
	Isoform 2. Checksum: 1D7F3F31EB89279B	FASTA	260	29,156
	10 20 30 40 50 60 MKVELLPALT DNYMYLLIDE DTKEAAIVDP VQPQKVVETA RKHGVKLTTV LTTHHHWDHA 70 80 90 100 110 120 GGNEKLVKLE PGLKVYGGDD RIGALTHKVT HLSTLQVGSL HVKCLSTPCH TSGHICYFVT 130 140 150 160 170 180 KPNSPEPPAV FTGDTLFVAG CGKFYEGTAD EMYKALLEVL GRLPADTRVY CGHEYTINNL 190 200 210 220 230 240 KFARHVEPDN TAVREKLAWA KEKYSIGEPT VPSTIAEEFT YNPFMRVREK TVQQHAGETE 250 260 PVATMRAIRK EKDQFKMPRD

References

[1]	NIH - Mammalian Gene Collection (MGC) project Submitted (OCT-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA] (ISOFORMS 1 AND 2). Strain: Hereford. Tissue: Heart ventricle.

Cross-references

Sequence databases
EMBL GenBank DDBJ	BC107545 mRNA. Translation: AAI07546.1.
IPI	IPI00718599. IPI00944423.
RefSeq	NP_001030351.1.
UniGene	Bt.20141
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B7M2.
Genome annotation databases
Ensembl	ENSBTAT00000026635; ENSBTAP00000026635; ENSBTAG00000019998; Bos taurus. [Genome view]
GeneID	509274.
KEGG	bta:509274.
Organism-specific databases
CTD	509274.
Phylogenomic databases
eggNOG	maNOG05483.
HOVERGEN	Q3B7M2.
InParanoid	Q3B7M2.
OMA	GSLNVKC.
OrthoDB	EOG9PRW91.
PhylomeDB	Q3B7M2.
Enzyme and pathway databases
BRENDA	3.1.2.6. 251.
Family and domain databases
InterPro	IPR001279. Blactmase-like. IPR017782. Hydroxyacylglutathione_Hdrlase. [Graphical view]
SMART	SM00849. Lactamase_B. 1 hit. [Graphical view]
TIGRFAMs	TIGR03413. GSH_gloB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

GLO2_BOVIN

Accession

Primary (citable) accession number: Q3B7M2

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 13, 2007
Last sequence update:	September 1, 2009
Last modified:	February 9, 2010
This is version 32 of the entry and version 3 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HPI (Human Proteome Initiative)