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Protein

DNA-directed RNA polymerase I subunit RPA43

Gene

TWISTNB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

BioCyciZFISH:HS12611-MONOMER.
ReactomeiR-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA43
Alternative name(s):
Twist neighbor protein
Gene namesi
Name:TWISTNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:18027. TWISTNB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

OpenTargetsiENSG00000105849.
PharmGKBiPA134878254.

Polymorphism and mutation databases

BioMutaiTWISTNB.
DMDMi121942339.

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002886321 – 338DNA-directed RNA polymerase I subunit RPA43Add BLAST338

Amino acid modifications

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Modified residuei242PhosphoserineCombined sources1
Modified residuei304PhosphoserineCombined sources1
Modified residuei316PhosphoserineCombined sources1
Modified residuei322PhosphothreonineCombined sources1
Modified residuei328PhosphoserineCombined sources1

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3B726.
MaxQBiQ3B726.
PaxDbiQ3B726.
PeptideAtlasiQ3B726.
PRIDEiQ3B726.

PTM databases

iPTMnetiQ3B726.
PhosphoSitePlusiQ3B726.

Expressioni

Tissue specificityi

Widely expressed. Expressed in all fetal and adult tissues tested, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues.1 Publication

Gene expression databases

BgeeiENSG00000105849.
CleanExiHS_TWISTNB.
GenevisibleiQ3B726. HS.

Organism-specific databases

HPAiHPA019071.
HPA022416.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity). Interacts with RRN3/TIF-IA.By similarity1 Publication

Protein-protein interaction databases

BioGridi128759. 20 interactors.
IntActiQ3B726. 6 interactors.
STRINGi9606.ENSP00000222567.

Structurei

3D structure databases

ProteinModelPortaliQ3B726.
SMRiQ3B726.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Compositional biasi224 – 334Lys-richAdd BLAST111

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4134. Eukaryota.
ENOG4111JD4. LUCA.
GeneTreeiENSGT00390000005553.
HOGENOMiHOG000060204.
HOVERGENiHBG108416.
InParanoidiQ3B726.
KOiK03004.
OMAiWLTMEIN.
OrthoDBiEOG091G0JWK.
PhylomeDBiQ3B726.
TreeFamiTF325602.

Family and domain databases

InterProiIPR005576. RNA_pol_Rpb7_N.
[Graphical view]
PfamiPF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3B726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGCSEAPR PAAASDGSLV GQAGVLPCLE LPTYAAACAL VNSRYSCLVA
60 70 80 90 100
GPHQRHIALS PRYLNRKRTG IREQLDAELL RYSESLLGVP IAYDNIKVVG
110 120 130 140 150
ELGDIYDDQG HIHLNIEADF VIFCPEPGQK LMGIVNKVSS SHIGCLVHGC
160 170 180 190 200
FNASIPKPEQ LSAEQWQTME INMGDELEFE VFRLDSDAAG VFCIRGKLNI
210 220 230 240 250
TSLQFKRSEV SEEVTENGTE EAAKKPKKKK KKKDPETYEV DSGTTKLADD
260 270 280 290 300
ADDTPMEESA LQNTNNANGI WEEEPKKKKK KKKHQEVQDQ DPVFQGSDSS
310 320 330
GYQSDHKKKK KKRKHSEEAE FTPPLKCSPK RKGKSNFL
Length:338
Mass (Da):37,432
Last modified:November 22, 2005 - v1
Checksum:i6DA3829E74E5D25C
GO

Sequence cautioni

The sequence AAH14574 differs from that shown. Reason: Frameshift at position 224.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301329 mRNA. Translation: BAH13460.1.
CH236948 Genomic DNA. Translation: EAL24277.1.
CH471073 Genomic DNA. Translation: EAW93714.1.
BC014574 mRNA. Translation: AAH14574.1. Frameshift.
BC130298 mRNA. Translation: AAI30299.1.
BC130300 mRNA. Translation: AAI30301.1.
BK000492 Genomic DNA. Translation: DAA05732.1.
CCDSiCCDS34606.1.
RefSeqiNP_001002926.1. NM_001002926.1.
UniGeneiHs.353035.

Genome annotation databases

EnsembliENST00000222567; ENSP00000222567; ENSG00000105849.
GeneIDi221830.
KEGGihsa:221830.
UCSCiuc003sup.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301329 mRNA. Translation: BAH13460.1.
CH236948 Genomic DNA. Translation: EAL24277.1.
CH471073 Genomic DNA. Translation: EAW93714.1.
BC014574 mRNA. Translation: AAH14574.1. Frameshift.
BC130298 mRNA. Translation: AAI30299.1.
BC130300 mRNA. Translation: AAI30301.1.
BK000492 Genomic DNA. Translation: DAA05732.1.
CCDSiCCDS34606.1.
RefSeqiNP_001002926.1. NM_001002926.1.
UniGeneiHs.353035.

3D structure databases

ProteinModelPortaliQ3B726.
SMRiQ3B726.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128759. 20 interactors.
IntActiQ3B726. 6 interactors.
STRINGi9606.ENSP00000222567.

PTM databases

iPTMnetiQ3B726.
PhosphoSitePlusiQ3B726.

Polymorphism and mutation databases

BioMutaiTWISTNB.
DMDMi121942339.

Proteomic databases

EPDiQ3B726.
MaxQBiQ3B726.
PaxDbiQ3B726.
PeptideAtlasiQ3B726.
PRIDEiQ3B726.

Protocols and materials databases

DNASUi221830.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222567; ENSP00000222567; ENSG00000105849.
GeneIDi221830.
KEGGihsa:221830.
UCSCiuc003sup.2. human.

Organism-specific databases

CTDi221830.
GeneCardsiTWISTNB.
HGNCiHGNC:18027. TWISTNB.
HPAiHPA019071.
HPA022416.
MIMi608312. gene.
neXtProtiNX_Q3B726.
OpenTargetsiENSG00000105849.
PharmGKBiPA134878254.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4134. Eukaryota.
ENOG4111JD4. LUCA.
GeneTreeiENSGT00390000005553.
HOGENOMiHOG000060204.
HOVERGENiHBG108416.
InParanoidiQ3B726.
KOiK03004.
OMAiWLTMEIN.
OrthoDBiEOG091G0JWK.
PhylomeDBiQ3B726.
TreeFamiTF325602.

Enzyme and pathway databases

BioCyciZFISH:HS12611-MONOMER.
ReactomeiR-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

GenomeRNAii221830.
PROiQ3B726.
SOURCEiSearch...

Gene expression databases

BgeeiENSG00000105849.
CleanExiHS_TWISTNB.
GenevisibleiQ3B726. HS.

Family and domain databases

InterProiIPR005576. RNA_pol_Rpb7_N.
[Graphical view]
PfamiPF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Entry informationi

Entry nameiRPA43_HUMAN
AccessioniPrimary (citable) accession number: Q3B726
Secondary accession number(s): A0PJ45, B7Z724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 100 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.