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Protein

DNA-directed RNA polymerase I subunit RPA43

Gene

TWISTNB

Organism
Homo sapiens (Human)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA using the four ribonucleoside triphosphates as substrates. Component of RNA polymerase I which synthesizes ribosomal RNA precursors. Through its association with RRN3/TIF-IA may be involved in recruitment of Pol I to rDNA promoters.

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Biological processi

Transcription

Enzyme and pathway databases

ReactomeiR-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.

Names & Taxonomyi

Protein namesi
Recommended name:
DNA-directed RNA polymerase I subunit RPA43
Alternative name(s):
Twist neighbor protein
Gene namesi
Name:TWISTNB
OrganismiHomo sapiens (Human)
Taxonomic identifieri9606 [NCBI]
Taxonomic lineageiEukaryotaMetazoaChordataCraniataVertebrataEuteleostomiMammaliaEutheriaEuarchontogliresPrimatesHaplorrhiniCatarrhiniHominidaeHomo
Proteomesi
  • UP000005640 Componenti: Chromosome 7

Organism-specific databases

HGNCiHGNC:18027. TWISTNB.

Subcellular locationi

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

DNA-directed RNA polymerase, Nucleus

Pathology & Biotechi

Organism-specific databases

PharmGKBiPA134878254.

Polymorphism and mutation databases

BioMutaiTWISTNB.
DMDMi121942339.

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 338338DNA-directed RNA polymerase I subunit RPA43PRO_0000288632Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei242 – 2421PhosphoserineCombined sources
Modified residuei304 – 3041PhosphoserineCombined sources
Modified residuei316 – 3161PhosphoserineCombined sources
Modified residuei322 – 3221PhosphothreonineCombined sources
Modified residuei328 – 3281PhosphoserineCombined sources

Keywords - PTMi

Phosphoprotein

Proteomic databases

EPDiQ3B726.
MaxQBiQ3B726.
PaxDbiQ3B726.
PeptideAtlasiQ3B726.
PRIDEiQ3B726.

PTM databases

iPTMnetiQ3B726.
PhosphoSiteiQ3B726.

Expressioni

Tissue specificityi

Widely expressed. Expressed in all fetal and adult tissues tested, with highest expression in fetal lung, liver, and kidney, and low expression in all adult tissues.1 Publication

Gene expression databases

BgeeiQ3B726.
CleanExiHS_TWISTNB.
GenevisibleiQ3B726. HS.

Organism-specific databases

HPAiHPA019071.
HPA022416.

Interactioni

Subunit structurei

Component of the RNA polymerase I (Pol I) complex consisting of at least 13 subunits (By similarity). Interacts with RRN3/TIF-IA.By similarity1 Publication

Protein-protein interaction databases

BioGridi128759. 20 interactions.
IntActiQ3B726. 6 interactions.
STRINGi9606.ENSP00000222567.

Structurei

3D structure databases

ProteinModelPortaliQ3B726.
SMRiQ3B726. Positions 42-157.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Compositional bias

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Compositional biasi224 – 334111Lys-richAdd
BLAST

Sequence similaritiesi

Phylogenomic databases

eggNOGiKOG4134. Eukaryota.
ENOG4111JD4. LUCA.
GeneTreeiENSGT00390000005553.
HOGENOMiHOG000060204.
HOVERGENiHBG108416.
InParanoidiQ3B726.
KOiK03004.
OMAiWLTMEIN.
OrthoDBiEOG7KWSKB.
PhylomeDBiQ3B726.
TreeFamiTF325602.

Family and domain databases

InterProiIPR005576. RNA_pol_Rpb7_N.
[Graphical view]
PfamiPF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3B726-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAAGCSEAPR PAAASDGSLV GQAGVLPCLE LPTYAAACAL VNSRYSCLVA
60 70 80 90 100
GPHQRHIALS PRYLNRKRTG IREQLDAELL RYSESLLGVP IAYDNIKVVG
110 120 130 140 150
ELGDIYDDQG HIHLNIEADF VIFCPEPGQK LMGIVNKVSS SHIGCLVHGC
160 170 180 190 200
FNASIPKPEQ LSAEQWQTME INMGDELEFE VFRLDSDAAG VFCIRGKLNI
210 220 230 240 250
TSLQFKRSEV SEEVTENGTE EAAKKPKKKK KKKDPETYEV DSGTTKLADD
260 270 280 290 300
ADDTPMEESA LQNTNNANGI WEEEPKKKKK KKKHQEVQDQ DPVFQGSDSS
310 320 330
GYQSDHKKKK KKRKHSEEAE FTPPLKCSPK RKGKSNFL
Length:338
Mass (Da):37,432
Last modified:November 22, 2005 - v1
Checksum:i6DA3829E74E5D25C
GO

Sequence cautioni

The sequence AAH14574.1 differs from that shown. Reason: Frameshift at position 224. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301329 mRNA. Translation: BAH13460.1.
CH236948 Genomic DNA. Translation: EAL24277.1.
CH471073 Genomic DNA. Translation: EAW93714.1.
BC014574 mRNA. Translation: AAH14574.1. Frameshift.
BC130298 mRNA. Translation: AAI30299.1.
BC130300 mRNA. Translation: AAI30301.1.
BK000492 Genomic DNA. Translation: DAA05732.1.
CCDSiCCDS34606.1.
RefSeqiNP_001002926.1. NM_001002926.1.
UniGeneiHs.353035.

Genome annotation databases

EnsembliENST00000222567; ENSP00000222567; ENSG00000105849.
GeneIDi221830.
KEGGihsa:221830.
UCSCiuc003sup.2. human.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AK301329 mRNA. Translation: BAH13460.1.
CH236948 Genomic DNA. Translation: EAL24277.1.
CH471073 Genomic DNA. Translation: EAW93714.1.
BC014574 mRNA. Translation: AAH14574.1. Frameshift.
BC130298 mRNA. Translation: AAI30299.1.
BC130300 mRNA. Translation: AAI30301.1.
BK000492 Genomic DNA. Translation: DAA05732.1.
CCDSiCCDS34606.1.
RefSeqiNP_001002926.1. NM_001002926.1.
UniGeneiHs.353035.

3D structure databases

ProteinModelPortaliQ3B726.
SMRiQ3B726. Positions 42-157.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

BioGridi128759. 20 interactions.
IntActiQ3B726. 6 interactions.
STRINGi9606.ENSP00000222567.

PTM databases

iPTMnetiQ3B726.
PhosphoSiteiQ3B726.

Polymorphism and mutation databases

BioMutaiTWISTNB.
DMDMi121942339.

Proteomic databases

EPDiQ3B726.
MaxQBiQ3B726.
PaxDbiQ3B726.
PeptideAtlasiQ3B726.
PRIDEiQ3B726.

Protocols and materials databases

DNASUi221830.
Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsembliENST00000222567; ENSP00000222567; ENSG00000105849.
GeneIDi221830.
KEGGihsa:221830.
UCSCiuc003sup.2. human.

Organism-specific databases

CTDi221830.
GeneCardsiTWISTNB.
HGNCiHGNC:18027. TWISTNB.
HPAiHPA019071.
HPA022416.
MIMi608312. gene.
neXtProtiNX_Q3B726.
PharmGKBiPA134878254.
GenAtlasiSearch...

Phylogenomic databases

eggNOGiKOG4134. Eukaryota.
ENOG4111JD4. LUCA.
GeneTreeiENSGT00390000005553.
HOGENOMiHOG000060204.
HOVERGENiHBG108416.
InParanoidiQ3B726.
KOiK03004.
OMAiWLTMEIN.
OrthoDBiEOG7KWSKB.
PhylomeDBiQ3B726.
TreeFamiTF325602.

Enzyme and pathway databases

ReactomeiR-HSA-427413. NoRC negatively regulates rRNA expression.
R-HSA-5250924. B-WICH complex positively regulates rRNA expression.
R-HSA-73762. RNA Polymerase I Transcription Initiation.
R-HSA-73772. RNA Polymerase I Promoter Escape.
R-HSA-73777. RNA Polymerase I Chain Elongation.
R-HSA-73863. RNA Polymerase I Transcription Termination.

Miscellaneous databases

GenomeRNAii221830.
PROiQ3B726.
SOURCEiSearch...

Gene expression databases

BgeeiQ3B726.
CleanExiHS_TWISTNB.
GenevisibleiQ3B726. HS.

Family and domain databases

InterProiIPR005576. RNA_pol_Rpb7_N.
[Graphical view]
PfamiPF03876. SHS2_Rpb7-N. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Complete sequencing and characterization of 21,243 full-length human cDNAs."
    Ota T., Suzuki Y., Nishikawa T., Otsuki T., Sugiyama T., Irie R., Wakamatsu A., Hayashi K., Sato H., Nagai K., Kimura K., Makita H., Sekine M., Obayashi M., Nishi T., Shibahara T., Tanaka T., Ishii S.
    , Yamamoto J., Saito K., Kawai Y., Isono Y., Nakamura Y., Nagahari K., Murakami K., Yasuda T., Iwayanagi T., Wagatsuma M., Shiratori A., Sudo H., Hosoiri T., Kaku Y., Kodaira H., Kondo H., Sugawara M., Takahashi M., Kanda K., Yokoi T., Furuya T., Kikkawa E., Omura Y., Abe K., Kamihara K., Katsuta N., Sato K., Tanikawa M., Yamazaki M., Ninomiya K., Ishibashi T., Yamashita H., Murakawa K., Fujimori K., Tanai H., Kimata M., Watanabe M., Hiraoka S., Chiba Y., Ishida S., Ono Y., Takiguchi S., Watanabe S., Yosida M., Hotuta T., Kusano J., Kanehori K., Takahashi-Fujii A., Hara H., Tanase T.-O., Nomura Y., Togiya S., Komai F., Hara R., Takeuchi K., Arita M., Imose N., Musashino K., Yuuki H., Oshima A., Sasaki N., Aotsuka S., Yoshikawa Y., Matsunawa H., Ichihara T., Shiohata N., Sano S., Moriya S., Momiyama H., Satoh N., Takami S., Terashima Y., Suzuki O., Nakagawa S., Senoh A., Mizoguchi H., Goto Y., Shimizu F., Wakebe H., Hishigaki H., Watanabe T., Sugiyama A., Takemoto M., Kawakami B., Yamazaki M., Watanabe K., Kumagai A., Itakura S., Fukuzumi Y., Fujimori Y., Komiyama M., Tashiro H., Tanigami A., Fujiwara T., Ono T., Yamada K., Fujii Y., Ozaki K., Hirao M., Ohmori Y., Kawabata A., Hikiji T., Kobatake N., Inagaki H., Ikema Y., Okamoto S., Okitani R., Kawakami T., Noguchi S., Itoh T., Shigeta K., Senba T., Matsumura K., Nakajima Y., Mizuno T., Morinaga M., Sasaki M., Togashi T., Oyama M., Hata H., Watanabe M., Komatsu T., Mizushima-Sugano J., Satoh T., Shirai Y., Takahashi Y., Nakagawa K., Okumura K., Nagase T., Nomura N., Kikuchi H., Masuho Y., Yamashita R., Nakai K., Yada T., Nakamura Y., Ohara O., Isogai T., Sugano S.
    Nat. Genet. 36:40-45(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Synovium.
  2. "Human chromosome 7: DNA sequence and biology."
    Scherer S.W., Cheung J., MacDonald J.R., Osborne L.R., Nakabayashi K., Herbrick J.-A., Carson A.R., Parker-Katiraee L., Skaug J., Khaja R., Zhang J., Hudek A.K., Li M., Haddad M., Duggan G.E., Fernandez B.A., Kanematsu E., Gentles S.
    , Christopoulos C.C., Choufani S., Kwasnicka D., Zheng X.H., Lai Z., Nusskern D.R., Zhang Q., Gu Z., Lu F., Zeesman S., Nowaczyk M.J., Teshima I., Chitayat D., Shuman C., Weksberg R., Zackai E.H., Grebe T.A., Cox S.R., Kirkpatrick S.J., Rahman N., Friedman J.M., Heng H.H.Q., Pelicci P.G., Lo-Coco F., Belloni E., Shaffer L.G., Pober B., Morton C.C., Gusella J.F., Bruns G.A.P., Korf B.R., Quade B.J., Ligon A.H., Ferguson H., Higgins A.W., Leach N.T., Herrick S.R., Lemyre E., Farra C.G., Kim H.-G., Summers A.M., Gripp K.W., Roberts W., Szatmari P., Winsor E.J.T., Grzeschik K.-H., Teebi A., Minassian B.A., Kere J., Armengol L., Pujana M.A., Estivill X., Wilson M.D., Koop B.F., Tosi S., Moore G.E., Boright A.P., Zlotorynski E., Kerem B., Kroisel P.M., Petek E., Oscier D.G., Mould S.J., Doehner H., Doehner K., Rommens J.M., Vincent J.B., Venter J.C., Li P.W., Mural R.J., Adams M.D., Tsui L.-C.
    Science 300:767-772(2003) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  3. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
  4. "The status, quality, and expansion of the NIH full-length cDNA project: the Mammalian Gene Collection (MGC)."
    The MGC Project Team
    Genome Res. 14:2121-2127(2004) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE MRNA].
    Tissue: Urinary bladder.
  5. "Identification and characterisation of the gene TWIST NEIGHBOR (TWISTNB) located in the microdeletion syndrome 7p21 region."
    Kosan C., Kunz J.
    Cytogenet. Genome Res. 97:167-170(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION, TISSUE SPECIFICITY.
  6. "Multiple interactions between RNA polymerase I, TIF-IA and TAF(I) subunits regulate preinitiation complex assembly at the ribosomal gene promoter."
    Yuan X., Zhao J., Zentgraf H., Hoffmann-Rohrer U., Grummt I.
    EMBO Rep. 3:1082-1087(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: INTERACTION WITH RRN3.
  7. "Global, in vivo, and site-specific phosphorylation dynamics in signaling networks."
    Olsen J.V., Blagoev B., Gnad F., Macek B., Kumar C., Mortensen P., Mann M.
    Cell 127:635-648(2006) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  8. "Combining protein-based IMAC, peptide-based IMAC, and MudPIT for efficient phosphoproteomic analysis."
    Cantin G.T., Yi W., Lu B., Park S.K., Xu T., Lee J.-D., Yates J.R. III
    J. Proteome Res. 7:1346-1351(2008) [PubMed] [Europe PMC] [Abstract]
    Cited for: IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  9. Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  10. "Quantitative phosphoproteomics reveals widespread full phosphorylation site occupancy during mitosis."
    Olsen J.V., Vermeulen M., Santamaria A., Kumar C., Miller M.L., Jensen L.J., Gnad F., Cox J., Jensen T.S., Nigg E.A., Brunak S., Mann M.
    Sci. Signal. 3:RA3-RA3(2010) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma.
  11. "System-wide temporal characterization of the proteome and phosphoproteome of human embryonic stem cell differentiation."
    Rigbolt K.T., Prokhorova T.A., Akimov V., Henningsen J., Johansen P.T., Kratchmarova I., Kassem M., Mann M., Olsen J.V., Blagoev B.
    Sci. Signal. 4:RS3-RS3(2011) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-316, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
  12. "Toward a comprehensive characterization of a human cancer cell phosphoproteome."
    Zhou H., Di Palma S., Preisinger C., Peng M., Polat A.N., Heck A.J., Mohammed S.
    J. Proteome Res. 12:260-271(2013) [PubMed] [Europe PMC] [Abstract]
    Cited for: PHOSPHORYLATION [LARGE SCALE ANALYSIS] AT SER-242; SER-304; SER-316; THR-322 AND SER-328, IDENTIFICATION BY MASS SPECTROMETRY [LARGE SCALE ANALYSIS].
    Tissue: Cervix carcinoma and Erythroleukemia.

Entry informationi

Entry nameiRPA43_HUMAN
AccessioniPrimary (citable) accession number: Q3B726
Secondary accession number(s): A0PJ45, B7Z724
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 22, 2005
Last modified: July 6, 2016
This is version 97 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programChordata Protein Annotation Program
DisclaimerAny medical or genetic information present in this entry is provided for research, educational and informational purposes only. It is not in any way intended to be used as a substitute for professional medical advice, diagnosis, treatment or care.

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Human chromosome 7
    Human chromosome 7: entries, gene names and cross-references to MIM
  2. MIM cross-references
    Online Mendelian Inheritance in Man (MIM) cross-references in UniProtKB/Swiss-Prot
  3. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.