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Protein

Callose synthase 5

Gene

CALS5

Organism
Arabidopsis thaliana (Mouse-ear cress)
Status
Reviewed-Annotation score: Annotation score: 5 out of 5-Experimental evidence at protein leveli

Functioni

Required for the formation of the callose wall separating the tetraspores (interstitial wall) and surrounding the pollen mother cells (pheripheral wall). Required for exine formation on pollen wall. May be involved in callose synthesis during pollen tube growth. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals.2 Publications

Catalytic activityi

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

GO - Molecular functioni

GO - Biological processi

  • (1->3)-beta-D-glucan biosynthetic process Source: TAIR
  • cell wall organization Source: UniProtKB-KW
  • microsporogenesis Source: TAIR
  • pollen germination Source: TAIR
  • pollen tube growth Source: TAIR
  • pollen wall assembly Source: TAIR
  • regulation of cell shape Source: UniProtKB-KW
  • regulation of pollen tube growth Source: TAIR
Complete GO annotation...

Keywords - Molecular functioni

Glycosyltransferase, Transferase

Keywords - Biological processi

Cell shape, Cell wall biogenesis/degradation

Enzyme and pathway databases

BioCyciARA:AT2G13680-MONOMER.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.
TCDBi9.B.119.1.2. the glycan synthase, fks1 (fks1) family.

Names & Taxonomyi

Protein namesi
Recommended name:
Callose synthase 5 (EC:2.4.1.34)
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 2
Protein LESS ADHERENT POLLEN 1
Gene namesi
Name:CALS5
Synonyms:GSL2, LAP1
Ordered Locus Names:At2g13680
ORF Names:F13J11.3, T10F5.22
OrganismiArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifieri3702 [NCBI]
Taxonomic lineageiEukaryotaViridiplantaeStreptophytaEmbryophytaTracheophytaSpermatophytaMagnoliophytaeudicotyledonsGunneridaePentapetalaerosidsmalvidsBrassicalesBrassicaceaeCamelineaeArabidopsis
Proteomesi
  • UP000006548 Componenti: Chromosome 2

Organism-specific databases

TAIRiAT2G13680.

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Topological domaini1 – 481481CytoplasmicSequence analysisAdd
BLAST
Transmembranei482 – 50221HelicalSequence analysisAdd
BLAST
Topological domaini503 – 52119ExtracellularSequence analysisAdd
BLAST
Transmembranei522 – 54221HelicalSequence analysisAdd
BLAST
Topological domaini543 – 55917CytoplasmicSequence analysisAdd
BLAST
Transmembranei560 – 58021HelicalSequence analysisAdd
BLAST
Topological domaini581 – 60121ExtracellularSequence analysisAdd
BLAST
Transmembranei602 – 62221HelicalSequence analysisAdd
BLAST
Topological domaini623 – 65836CytoplasmicSequence analysisAdd
BLAST
Transmembranei659 – 67921HelicalSequence analysisAdd
BLAST
Topological domaini680 – 71940ExtracellularSequence analysisAdd
BLAST
Transmembranei720 – 74021HelicalSequence analysisAdd
BLAST
Topological domaini741 – 1486746CytoplasmicSequence analysisAdd
BLAST
Transmembranei1487 – 150721HelicalSequence analysisAdd
BLAST
Topological domaini1508 – 153528ExtracellularSequence analysisAdd
BLAST
Transmembranei1536 – 155621HelicalSequence analysisAdd
BLAST
Topological domaini1557 – 156610CytoplasmicSequence analysis
Transmembranei1567 – 158721HelicalSequence analysisAdd
BLAST
Topological domaini1588 – 163043ExtracellularSequence analysisAdd
BLAST
Transmembranei1631 – 165121HelicalSequence analysisAdd
BLAST
Topological domaini1652 – 16576CytoplasmicSequence analysis
Transmembranei1658 – 167821HelicalSequence analysisAdd
BLAST
Topological domaini1679 – 173254ExtracellularSequence analysisAdd
BLAST
Transmembranei1733 – 175523HelicalSequence analysisAdd
BLAST
Topological domaini1756 – 176611CytoplasmicSequence analysisAdd
BLAST
Transmembranei1767 – 178721HelicalSequence analysisAdd
BLAST
Topological domaini1788 – 180316ExtracellularSequence analysisAdd
BLAST
Transmembranei1804 – 182421HelicalSequence analysisAdd
BLAST
Topological domaini1825 – 18251CytoplasmicSequence analysis
Transmembranei1826 – 184621HelicalSequence analysisAdd
BLAST
Topological domaini1847 – 187327ExtracellularSequence analysisAdd
BLAST
Transmembranei1874 – 189421HelicalSequence analysisAdd
BLAST
Topological domaini1895 – 192329CytoplasmicSequence analysisAdd
BLAST

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Disruption phenotypei

Plants develop deformed and inviable pollen grains which do not have exin.1 Publication

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi651 – 6511G → I in cals5-4; loss of exine. 2 Publications

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 19231923Callose synthase 5PRO_0000334577Add
BLAST

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Glycosylationi1710 – 17101N-linked (GlcNAc...)Sequence analysis

Keywords - PTMi

Glycoprotein

Proteomic databases

PaxDbiQ3B724.
PRIDEiQ3B724.

Expressioni

Developmental stagei

Expressed throughout pollen development, both in pollen mother cells and in developing and mature pollen grains. Expressed in growing pollen tube.2 Publications

Gene expression databases

GenevisibleiQ3B724. AT.

Interactioni

Protein-protein interaction databases

STRINGi3702.AT2G13680.1.

Structurei

3D structure databases

ProteinModelPortaliQ3B724.
SMRiQ3B724. Positions 36-161.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the glycosyltransferase 48 family.Curated

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ3B724.
KOiK11000.
OMAiFPENYRM.
PhylomeDBiQ3B724.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3B724-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MAQSSTSHDS GPQGLMRRPS RSAATTVSIE VFDHEVVPAS LGTIAPILRV
60 70 80 90 100
AAEIEHERPR VAYLCRFYAF EKAHRLDPSS GGRGVRQFKT LLFQRLERDN
110 120 130 140 150
ASSLASRVKK TDGREVESFY QQYYEHYVRA LDQGDQADRA QLGKAYQTAG
160 170 180 190 200
VLFEVLMAVN KSEKVEAVAP EIIAAARDVQ EKNEIYAPYN ILPLDSAGAS
210 220 230 240 250
QSVMQLEEVK AAVAALGNTR GLNWPSGFEQ HRKKTGNLDL LDWLRAMFGF
260 270 280 290 300
QRDNVRNQRE HLVCLFADNH IRLTPKPEPL NKLDDRAVDT VMSKLFKNYK
310 320 330 340 350
NWCKFLGRKH SLRLPQAAQD IQQRKILYMG LYLLIWGEAA NIRFMPECLC
360 370 380 390 400
YIFHNMAYEL HGLLAGNVSI VTGENIKPSY GGDDEAFLRK VITPIYRVVQ
410 420 430 440 450
TEANKNANGK AAHSDWSNYD DLNEYFWTPD CFSLGWPMRD DGDLFKSTRD
460 470 480 490 500
TTQGKKGSFR KAGRTGKSNF TETRTFWHIY HSFDRLWTFY LLALQAMIIL
510 520 530 540 550
AFERVELREI LRKDVLYALS SIFITAAFLR FLQSVLDVIL NFPGFHRWKF
560 570 580 590 600
TDVLRNILKI VVSLAWCVVL PLCYAQSVSF APGKLKQWLS FLPQVKGVPP
610 620 630 640 650
LYIMAVALYL LPNVLAAIMF IFPMLRRWIE NSDWHIFRLL LWWSQPRIYV
660 670 680 690 700
GRGMHESQIA LIKYTIFWLL LFCCKFAFSY FLQVKLLVKP TNAIMSIRHV
710 720 730 740 750
KYKWHEFFPN AEHNYGAVVS LWLPVILVYF MDTQIWYAIF STICGGVIGA
760 770 780 790 800
FDRLGEIRTL GMLRSRFQSL PGAFNTYLVP SDKTRRRGFS LSKRFAEVTA
810 820 830 840 850
ARRTEAAKFS QLWNEIISSF REEDLISDRE MDLLLVPYTS DPSLKLIQWP
860 870 880 890 900
PFLLASKIPI ALDMAAQFRT RDSDLWKRIC ADEYMKCAVI ECYESFKHVL
910 920 930 940 950
HTLVIGENEK RIIGIIIKEV ESNISKNSFL SNFRMAPLPA LCSKFVELVG
960 970 980 990 1000
ILKNADPAKR DTVVLLLQDM LEVVTRDMMQ NENRELVELG HTNKESGRQL
1010 1020 1030 1040 1050
FAGTDAKPAI LFPPVATAQW HEQISRLHLL LTVKESAMDV PTNLEAQRRI
1060 1070 1080 1090 1100
AFFTNSLFMD MPRAPRVRNM LSFSVLTPYY SEETVYSKND LEMENEDGVS
1110 1120 1130 1140 1150
VVYYLQKIFP DEWTNFLERL DCKDETSVLE SEENILQLRH WVSLRGQTLF
1160 1170 1180 1190 1200
RTVRGMMYYR RALKLQAFLD MANETEILAG YKAISEPTEE DKKSQRSLYT
1210 1220 1230 1240 1250
QLEAVADLKF TYVATCQNYG NQKRSGDRRA TDILNLMVNN PSLRVAYIDE
1260 1270 1280 1290 1300
VEEREGGKVQ KVFYSVLIKA VDNLDQEIYR IKLPGPAKIG EGKPENQNHA
1310 1320 1330 1340 1350
LIFTRGEALQ AIDMNQDHYL EEALKMRNLL EEFNEDHGVR APTILGFREH
1360 1370 1380 1390 1400
IFTGSVSSLA WFMSNQETSF VTIGQRVLAS PLKVRFHYGH PDVFDRIFHI
1410 1420 1430 1440 1450
TRGGISKASR GINLSEDIFA GFNSTLRRGN VTHHEYIQVG KGRDVGLNQI
1460 1470 1480 1490 1500
SLFEAKVACG NGEQTLSRDL YRLGHRFDFF RMMSCYFTTV GFYISSMIVV
1510 1520 1530 1540 1550
LTVYAFLYGR LYLSLSGVEE AIVKFAAAKG DSSLKAAMAS QSVVQLGLLM
1560 1570 1580 1590 1600
TLPMVMEIGL ERGFRTALSD LIIMQLQLAP VFFTFSLGTK VHYYGRTILH
1610 1620 1630 1640 1650
GGSKYRATGR GFVVKHEKFA ENYRMYSRSH FVKGMELMVL LICYRIYGKA
1660 1670 1680 1690 1700
AEDSVGYALV MGSTWFLVGS WLFAPFFFNP SGFEWQKIVD DWDDWNKWIS
1710 1720 1730 1740 1750
SRGGIGVPAN KSWESWWEEE QEHLLHSGFF GKFWEIFLSL RYFIYQYGIV
1760 1770 1780 1790 1800
YQLNLTKESR MGKQHSIIVY GLSWLVIVAV MIVLKIVSMG RKKFSADFQL
1810 1820 1830 1840 1850
MFRLLKLFLF IGSVVIVGML FHFLKLTVGD IMQSLLAFLP TGWALLQISQ
1860 1870 1880 1890 1900
VARPLMKTVG MWGSVKALAR GYEYIMGVVI FMPVTVLAWF PFVSEFQTRL
1910 1920
LFNQAFSRGL QIQRILAGGK KQK
Length:1,923
Mass (Da):220,661
Last modified:November 22, 2005 - v1
Checksum:iBDB264C8530D418C
GO

Sequence cautioni

The sequence AAM15250.1 differs from that shown. Reason: Erroneous gene model prediction. Curated
The sequence AAM15369.1 differs from that shown. Reason: Erroneous gene model prediction. Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY337762 mRNA. Translation: AAR00322.1.
AC006436 Genomic DNA. Translation: AAM15250.1. Sequence problems.
AC007063 Genomic DNA. Translation: AAM15369.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06254.1.
BK001470 mRNA. Translation: DAA01511.1.
RefSeqiNP_849953.2. NM_179622.3.
UniGeneiAt.52728.

Genome annotation databases

EnsemblPlantsiAT2G13680.1; AT2G13680.1; AT2G13680.
GeneIDi815852.
GrameneiAT2G13680.1; AT2G13680.1; AT2G13680.
KEGGiath:AT2G13680.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
AY337762 mRNA. Translation: AAR00322.1.
AC006436 Genomic DNA. Translation: AAM15250.1. Sequence problems.
AC007063 Genomic DNA. Translation: AAM15369.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06254.1.
BK001470 mRNA. Translation: DAA01511.1.
RefSeqiNP_849953.2. NM_179622.3.
UniGeneiAt.52728.

3D structure databases

ProteinModelPortaliQ3B724.
SMRiQ3B724. Positions 36-161.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi3702.AT2G13680.1.

Protein family/group databases

CAZyiGT48. Glycosyltransferase Family 48.
TCDBi9.B.119.1.2. the glycan synthase, fks1 (fks1) family.

Proteomic databases

PaxDbiQ3B724.
PRIDEiQ3B724.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsiAT2G13680.1; AT2G13680.1; AT2G13680.
GeneIDi815852.
GrameneiAT2G13680.1; AT2G13680.1; AT2G13680.
KEGGiath:AT2G13680.

Organism-specific databases

TAIRiAT2G13680.

Phylogenomic databases

eggNOGiKOG0916. Eukaryota.
ENOG410XQ8V. LUCA.
HOGENOMiHOG000029513.
InParanoidiQ3B724.
KOiK11000.
OMAiFPENYRM.
PhylomeDBiQ3B724.

Enzyme and pathway databases

BioCyciARA:AT2G13680-MONOMER.

Miscellaneous databases

PROiQ3B724.

Gene expression databases

GenevisibleiQ3B724. AT.

Family and domain databases

Gene3Di1.25.40.270. 1 hit.
InterProiIPR026899. FKS1-like_dom1.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1/CALS_N-dom.
[Graphical view]
PfamiPF14288. FKS1_dom1. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
SMARTiSM01205. FKS1_dom1. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. "Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth."
    Nishikawa S., Zinkl G.M., Swanson R.J., Maruyama D., Preuss D.
    BMC Plant Biol. 5:22-22(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, IDENTIFICATION, MUTAGENESIS OF GLY-651, DISRUPTION PHENOTYPE.
    Strain: cv. Landsberg erecta.
  2. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: cv. Columbia.
  3. The Arabidopsis Information Resource (TAIR)
    Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
    Cited for: GENOME REANNOTATION.
    Strain: cv. Columbia.
  4. "A cell plate-specific callose synthase and its interaction with phragmoplastin."
    Hong Z., Delauney A.J., Verma D.P.S.
    Plant Cell 13:755-768(2001) [PubMed] [Europe PMC] [Abstract]
    Cited for: GENE FAMILY, NOMENCLATURE.
  5. "Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis."
    Dong X., Hong Z., Sivaramakrishnan M., Mahfouz M., Verma D.P.S.
    Plant J. 42:315-328(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-651.
  6. "Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
    Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
    Plant Mol. Biol. 58:333-349(2005) [PubMed] [Europe PMC] [Abstract]
    Cited for: NOMENCLATURE.

Entry informationi

Entry nameiCALS5_ARATH
AccessioniPrimary (citable) accession number: Q3B724
Secondary accession number(s): Q8S8D4, Q8S8G9
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 22, 2005
Last modified: May 11, 2016
This is version 78 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Arabidopsis thaliana
    Arabidopsis thaliana: entries and gene names
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.