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Q3B724 (CALS5_ARATH) Reviewed, UniProtKB/Swiss-Prot

Last modified January 25, 2012. Version 47. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Callose synthase 5
EC=2.4.1.34
Alternative name(s):
1,3-beta-glucan synthase
Protein GLUCAN SYNTHASE-LIKE 2
Protein LESS ADHERENT POLLEN 1
Gene names
Name:CALS5
Synonyms:GSL2, LAP1
Ordered Locus Names:At2g13680
ORF Names:F13J11.3, T10F5.22
OrganismArabidopsis thaliana (Mouse-ear cress)
Taxonomic identifier3702 [NCBI]
Taxonomic lineageEukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › malvids › Brassicales › Brassicaceae › Camelineae › Arabidopsis

Protein attributes

Sequence length1923 AA.
Sequence statusComplete.
Protein existenceEvidence at protein level

General annotation (Comments)

Function

Required for the formation of the callose wall separating the tetraspores (interstitial wall) and surrounding the pollen mother cells (pheripheral wall). Required for exine formation on pollen wall. May be involved in callose synthesis during pollen tube growth. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.1 Ref.5

Catalytic activity

UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).

Subcellular location

Cell membrane; Multi-pass membrane protein Probable.

Developmental stage

Expressed throughout pollen development, both in pollen mother cells and in developing and mature pollen grains. Expressed in growing pollen tube. Ref.1 Ref.5

Disruption phenotype

Plants develop deformed and inviable pollen grains which do not have exin. Ref.1

Sequence similarities

Belongs to the glycosyltransferase 48 family.

Sequence caution

The sequence AAM15250.1 differs from that shown. Reason: Erroneous gene model prediction.

The sequence AAM15369.1 differs from that shown. Reason: Erroneous gene model prediction.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 19231923Callose synthase 5
PRO_0000334577

Regions

Topological domain1 – 481481Cytoplasmic Potential
Transmembrane482 – 50221Helical; Potential
Topological domain503 – 52119Extracellular Potential
Transmembrane522 – 54221Helical; Potential
Topological domain543 – 55917Cytoplasmic Potential
Transmembrane560 – 58021Helical; Potential
Topological domain581 – 60121Extracellular Potential
Transmembrane602 – 62221Helical; Potential
Topological domain623 – 65836Cytoplasmic Potential
Transmembrane659 – 67921Helical; Potential
Topological domain680 – 71940Extracellular Potential
Transmembrane720 – 74021Helical; Potential
Topological domain741 – 1486746Cytoplasmic Potential
Transmembrane1487 – 150721Helical; Potential
Topological domain1508 – 153528Extracellular Potential
Transmembrane1536 – 155621Helical; Potential
Topological domain1557 – 156610Cytoplasmic Potential
Transmembrane1567 – 158721Helical; Potential
Topological domain1588 – 163043Extracellular Potential
Transmembrane1631 – 165121Helical; Potential
Topological domain1652 – 16576Cytoplasmic Potential
Transmembrane1658 – 167821Helical; Potential
Topological domain1679 – 173254Extracellular Potential
Transmembrane1733 – 175523Helical; Potential
Topological domain1756 – 176611Cytoplasmic Potential
Transmembrane1767 – 178721Helical; Potential
Topological domain1788 – 180316Extracellular Potential
Transmembrane1804 – 182421Helical; Potential
Topological domain18251Cytoplasmic Potential
Transmembrane1826 – 184621Helical; Potential
Topological domain1847 – 187327Extracellular Potential
Transmembrane1874 – 189421Helical; Potential
Topological domain1895 – 192329Cytoplasmic Potential

Amino acid modifications

Glycosylation17101N-linked (GlcNAc...) Potential

Experimental info

Mutagenesis6511G → I in cals5-4; loss of exine. Ref.1 Ref.5

Sequences

Sequence LengthMass (Da)Tools
Q3B724 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: BDB264C8530D418C

FASTA1,923220,661
        10         20         30         40         50         60 
MAQSSTSHDS GPQGLMRRPS RSAATTVSIE VFDHEVVPAS LGTIAPILRV AAEIEHERPR 

        70         80         90        100        110        120 
VAYLCRFYAF EKAHRLDPSS GGRGVRQFKT LLFQRLERDN ASSLASRVKK TDGREVESFY 

       130        140        150        160        170        180 
QQYYEHYVRA LDQGDQADRA QLGKAYQTAG VLFEVLMAVN KSEKVEAVAP EIIAAARDVQ 

       190        200        210        220        230        240 
EKNEIYAPYN ILPLDSAGAS QSVMQLEEVK AAVAALGNTR GLNWPSGFEQ HRKKTGNLDL 

       250        260        270        280        290        300 
LDWLRAMFGF QRDNVRNQRE HLVCLFADNH IRLTPKPEPL NKLDDRAVDT VMSKLFKNYK 

       310        320        330        340        350        360 
NWCKFLGRKH SLRLPQAAQD IQQRKILYMG LYLLIWGEAA NIRFMPECLC YIFHNMAYEL 

       370        380        390        400        410        420 
HGLLAGNVSI VTGENIKPSY GGDDEAFLRK VITPIYRVVQ TEANKNANGK AAHSDWSNYD 

       430        440        450        460        470        480 
DLNEYFWTPD CFSLGWPMRD DGDLFKSTRD TTQGKKGSFR KAGRTGKSNF TETRTFWHIY 

       490        500        510        520        530        540 
HSFDRLWTFY LLALQAMIIL AFERVELREI LRKDVLYALS SIFITAAFLR FLQSVLDVIL 

       550        560        570        580        590        600 
NFPGFHRWKF TDVLRNILKI VVSLAWCVVL PLCYAQSVSF APGKLKQWLS FLPQVKGVPP 

       610        620        630        640        650        660 
LYIMAVALYL LPNVLAAIMF IFPMLRRWIE NSDWHIFRLL LWWSQPRIYV GRGMHESQIA 

       670        680        690        700        710        720 
LIKYTIFWLL LFCCKFAFSY FLQVKLLVKP TNAIMSIRHV KYKWHEFFPN AEHNYGAVVS 

       730        740        750        760        770        780 
LWLPVILVYF MDTQIWYAIF STICGGVIGA FDRLGEIRTL GMLRSRFQSL PGAFNTYLVP 

       790        800        810        820        830        840 
SDKTRRRGFS LSKRFAEVTA ARRTEAAKFS QLWNEIISSF REEDLISDRE MDLLLVPYTS 

       850        860        870        880        890        900 
DPSLKLIQWP PFLLASKIPI ALDMAAQFRT RDSDLWKRIC ADEYMKCAVI ECYESFKHVL 

       910        920        930        940        950        960 
HTLVIGENEK RIIGIIIKEV ESNISKNSFL SNFRMAPLPA LCSKFVELVG ILKNADPAKR 

       970        980        990       1000       1010       1020 
DTVVLLLQDM LEVVTRDMMQ NENRELVELG HTNKESGRQL FAGTDAKPAI LFPPVATAQW 

      1030       1040       1050       1060       1070       1080 
HEQISRLHLL LTVKESAMDV PTNLEAQRRI AFFTNSLFMD MPRAPRVRNM LSFSVLTPYY 

      1090       1100       1110       1120       1130       1140 
SEETVYSKND LEMENEDGVS VVYYLQKIFP DEWTNFLERL DCKDETSVLE SEENILQLRH 

      1150       1160       1170       1180       1190       1200 
WVSLRGQTLF RTVRGMMYYR RALKLQAFLD MANETEILAG YKAISEPTEE DKKSQRSLYT 

      1210       1220       1230       1240       1250       1260 
QLEAVADLKF TYVATCQNYG NQKRSGDRRA TDILNLMVNN PSLRVAYIDE VEEREGGKVQ 

      1270       1280       1290       1300       1310       1320 
KVFYSVLIKA VDNLDQEIYR IKLPGPAKIG EGKPENQNHA LIFTRGEALQ AIDMNQDHYL 

      1330       1340       1350       1360       1370       1380 
EEALKMRNLL EEFNEDHGVR APTILGFREH IFTGSVSSLA WFMSNQETSF VTIGQRVLAS 

      1390       1400       1410       1420       1430       1440 
PLKVRFHYGH PDVFDRIFHI TRGGISKASR GINLSEDIFA GFNSTLRRGN VTHHEYIQVG 

      1450       1460       1470       1480       1490       1500 
KGRDVGLNQI SLFEAKVACG NGEQTLSRDL YRLGHRFDFF RMMSCYFTTV GFYISSMIVV 

      1510       1520       1530       1540       1550       1560 
LTVYAFLYGR LYLSLSGVEE AIVKFAAAKG DSSLKAAMAS QSVVQLGLLM TLPMVMEIGL 

      1570       1580       1590       1600       1610       1620 
ERGFRTALSD LIIMQLQLAP VFFTFSLGTK VHYYGRTILH GGSKYRATGR GFVVKHEKFA 

      1630       1640       1650       1660       1670       1680 
ENYRMYSRSH FVKGMELMVL LICYRIYGKA AEDSVGYALV MGSTWFLVGS WLFAPFFFNP 

      1690       1700       1710       1720       1730       1740 
SGFEWQKIVD DWDDWNKWIS SRGGIGVPAN KSWESWWEEE QEHLLHSGFF GKFWEIFLSL 

      1750       1760       1770       1780       1790       1800 
RYFIYQYGIV YQLNLTKESR MGKQHSIIVY GLSWLVIVAV MIVLKIVSMG RKKFSADFQL 

      1810       1820       1830       1840       1850       1860 
MFRLLKLFLF IGSVVIVGML FHFLKLTVGD IMQSLLAFLP TGWALLQISQ VARPLMKTVG 

      1870       1880       1890       1900       1910       1920 
MWGSVKALAR GYEYIMGVVI FMPVTVLAWF PFVSEFQTRL LFNQAFSRGL QIQRILAGGK 


KQK

« Hide

References

« Hide 'large scale' references
[1]"Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth."
Nishikawa S., Zinkl G.M., Swanson R.J., Maruyama D., Preuss D.
BMC Plant Biol. 5:22-22(2005) [PubMed: 16212660] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, IDENTIFICATION, MUTAGENESIS OF GLY-651, DISRUPTION PHENOTYPE.
Strain: cv. Landsberg erecta.
[2]"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana."
Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. expand/collapse author list , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C.
Nature 402:761-768(1999) [PubMed: 10617197] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: cv. Columbia.
[3]The Arabidopsis Information Resource (TAIR)
Submitted (APR-2011) to the EMBL/GenBank/DDBJ databases
Cited for: GENOME REANNOTATION.
Strain: cv. Columbia.
[4]"A cell plate-specific callose synthase and its interaction with phragmoplastin."
Hong Z., Delauney A.J., Verma D.P.S.
Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract]
Cited for: GENE FAMILY AND NOMENCLATURE.
[5]"Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis."
Dong X., Hong Z., Sivaramakrishnan M., Mahfouz M., Verma D.P.S.
Plant J. 42:315-328(2005) [PubMed: 15842618] [Abstract]
Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-651.
[6]"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility."
Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E.
Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract]
Cited for: NOMENCLATURE.
+Additional computationally mapped references.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ		AY337762 mRNA. Translation: AAR00322.1.
AC006436 Genomic DNA. Translation: AAM15250.1. Sequence problems.
AC007063 Genomic DNA. Translation: AAM15369.1. Sequence problems.
CP002685 Genomic DNA. Translation: AEC06254.1.
BK001470 mRNA. Translation: DAA01511.1.
IPIIPI00516810.
RefSeqNP_849953.2. NM_179622.3.
UniGeneAt.52728.

3D structure databases

ProteinModelPortalQ3B724.
ModBaseSearch...

Protein family/group databases

CAZyGT48. Glycosyltransferase Family 48.

Proteomic databases

PRIDEQ3B724.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblPlantsAT2G13680.1; AT2G13680.1; AT2G13680.
GeneID815852.
GenomeReviewsGene locus AT2G13680 in contig CT485783_GR.
KEGGath:AT2G13680.
NMPDRfig|3702.1.peg.8414.

Organism-specific databases

TAIRAt2g13680.

Phylogenomic databases

eggNOGKOG0916.
GeneTreeEPGT00070000028111.
HOGENOMHBG316390.
InParanoidQ3B724.
OMARICADEY.
PhylomeDBQ3B724.
ProtClustDBCLSN2681240.

Gene expression databases

GenevestigatorQ3B724.

Family and domain databases

InterProIPR006745. DUF605.
IPR003440. Glyco_trans_48.
IPR023175. VPS_Vta1_N.
[Graphical view]
Gene3DG3DSA:1.25.40.270. G3DSA:1.25.40.270. 1 hit.
KOK11000.
PfamPF04652. DUF605. 1 hit.
PF02364. Glucan_synthase. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameCALS5_ARATH
AccessionPrimary (citable) accession number: Q3B724
Secondary accession number(s): Q8S8D4, Q8S8G9
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 22, 2005
Last modified: January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programPlant Protein Annotation Program

Relevant documents

Arabidopsis thaliana

Arabidopsis thaliana: entries and gene names

SIMILARITY comments

Index of protein domains and families

to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·Documents