Reviewed, UniProtKB/Swiss-Prot Q3B724 (CALS5_ARATH)

Names and origin

Protein names

Recommended name:
    Callose synthase 5
    EC=2.4.1.34
Alternative name(s):
    1,3-beta-glucan synthase
    Protein GLUCAN SYNTHASE-LIKE 2
    Protein LESS ADHERENT POLLEN 1

Gene names

Name:	CALS5
Synonyms:	GSL2, LAP1
Ordered Locus Names:	At2g13680
ORF Names:	F13J11.3, T10F5.22

Organism

Arabidopsis thaliana (Mouse-ear cress) [Complete proteome]

Taxonomic identifier

3702 [NCBI]

Taxonomic lineage

Eukaryota › Viridiplantae › Streptophyta › Embryophyta › Tracheophyta › Spermatophyta › Magnoliophyta › eudicotyledons › core eudicotyledons › rosids › eurosids II › Brassicales › Brassicaceae › Arabidopsis

Protein attributes

Sequence length	1923 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Evidence at protein level.

General annotation (Comments)

Function	Required for the formation of the callose wall separating the tetraspores (interstitial wall) and surrounding the pollen mother cells (pheripheral wall). Required for exine formation on pollen wall. May be involved in callose synthesis during pollen tube growth. During plant growth and development, callose is found as a transitory component of the cell plate in dividing cells, is a major component of pollen mother cell walls and pollen tubes, and is found as a structural component of plasmodesmatal canals. Ref.1 Ref.4
Catalytic activity	UDP-glucose + ((1->3)-beta-D-glucosyl)(n) = UDP + ((1->3)-beta-D-glucosyl)(n+1).
Subcellular location	Cell membrane; Multi-pass membrane protein Probable.
Developmental stage	Expressed throughout pollen development, both in pollen mother cells and in developing and mature pollen grains. Expressed in growing pollen tube. Ref.1 Ref.4
Disruption phenotype	Plants develop deformed and inviable pollen grains which do not have exin. Ref.1
Sequence similarities	Belongs to the glycosyltransferase 48 family.
Sequence caution	The sequence AAM15250.1 differs from that shown. Reason: Erroneous gene model prediction. The sequence AAM15369.1 differs from that shown. Reason: Erroneous gene model prediction.

Ontologies

Keywords
Biological process	Cell shape Cell wall biogenesis/degradation
Cellular component	Cell membrane Membrane
Domain	Transmembrane
Molecular function	Glycosyltransferase Transferase
PTM	Glycoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	1,3-beta-glucan biosynthetic process Ref.4 Inferred from mutant phenotype. Source: TAIR cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW microsporogenesis Ref.4 Inferred from mutant phenotype. Source: TAIR pollen wall assembly Ref.4 Inferred from mutant phenotype. Source: TAIR regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	1,3-beta-glucan synthase complex Inferred from electronic annotation. Source: InterPro integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	1,3-beta-glucan synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 1923	1923	Callose synthase 5		PRO_0000334577
Regions
	Topological domain	1 – 481	481	Cytoplasmic Potential
	Transmembrane	482 – 502	21	Potential
	Topological domain	503 – 521	19	Extracellular Potential
	Transmembrane	522 – 542	21	Potential
	Topological domain	543 – 559	17	Cytoplasmic Potential
	Transmembrane	560 – 580	21	Potential
	Topological domain	581 – 601	21	Extracellular Potential
	Transmembrane	602 – 622	21	Potential
	Topological domain	623 – 658	36	Cytoplasmic Potential
	Transmembrane	659 – 679	21	Potential
	Topological domain	680 – 719	40	Extracellular Potential
	Transmembrane	720 – 740	21	Potential
	Topological domain	741 – 1486	746	Cytoplasmic Potential
	Transmembrane	1487 – 1507	21	Potential
	Topological domain	1508 – 1535	28	Extracellular Potential
	Transmembrane	1536 – 1556	21	Potential
	Topological domain	1557 – 1566	10	Cytoplasmic Potential
	Transmembrane	1567 – 1587	21	Potential
	Topological domain	1588 – 1630	43	Extracellular Potential
	Transmembrane	1631 – 1651	21	Potential
	Topological domain	1652 – 1657	6	Cytoplasmic Potential
	Transmembrane	1658 – 1678	21	Potential
	Topological domain	1679 – 1732	54	Extracellular Potential
	Transmembrane	1733 – 1755	23	Potential
	Topological domain	1756 – 1766	11	Cytoplasmic Potential
	Transmembrane	1767 – 1787	21	Potential
	Topological domain	1788 – 1803	16	Extracellular Potential
	Transmembrane	1804 – 1824	21	Potential
	Topological domain	1825	1	Cytoplasmic Potential
	Transmembrane	1826 – 1846	21	Potential
	Topological domain	1847 – 1873	27	Extracellular Potential
	Transmembrane	1874 – 1894	21	Potential
	Topological domain	1895 – 1923	29	Cytoplasmic Potential
Amino acid modifications
	Glycosylation	1710	1	N-linked (GlcNAc...) Potential
Experimental info
	Mutagenesis	651	1	G → I in cals5-4; loss of exine. Ref.1 Ref.4

Sequences

	Sequence		Length	Mass (Da)
	Q3B724-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: BDB264C8530D418C	FASTA	1,923	220,661
	10 20 30 40 50 60 MAQSSTSHDS GPQGLMRRPS RSAATTVSIE VFDHEVVPAS LGTIAPILRV AAEIEHERPR 70 80 90 100 110 120 VAYLCRFYAF EKAHRLDPSS GGRGVRQFKT LLFQRLERDN ASSLASRVKK TDGREVESFY 130 140 150 160 170 180 QQYYEHYVRA LDQGDQADRA QLGKAYQTAG VLFEVLMAVN KSEKVEAVAP EIIAAARDVQ 190 200 210 220 230 240 EKNEIYAPYN ILPLDSAGAS QSVMQLEEVK AAVAALGNTR GLNWPSGFEQ HRKKTGNLDL 250 260 270 280 290 300 LDWLRAMFGF QRDNVRNQRE HLVCLFADNH IRLTPKPEPL NKLDDRAVDT VMSKLFKNYK 310 320 330 340 350 360 NWCKFLGRKH SLRLPQAAQD IQQRKILYMG LYLLIWGEAA NIRFMPECLC YIFHNMAYEL 370 380 390 400 410 420 HGLLAGNVSI VTGENIKPSY GGDDEAFLRK VITPIYRVVQ TEANKNANGK AAHSDWSNYD 430 440 450 460 470 480 DLNEYFWTPD CFSLGWPMRD DGDLFKSTRD TTQGKKGSFR KAGRTGKSNF TETRTFWHIY 490 500 510 520 530 540 HSFDRLWTFY LLALQAMIIL AFERVELREI LRKDVLYALS SIFITAAFLR FLQSVLDVIL 550 560 570 580 590 600 NFPGFHRWKF TDVLRNILKI VVSLAWCVVL PLCYAQSVSF APGKLKQWLS FLPQVKGVPP 610 620 630 640 650 660 LYIMAVALYL LPNVLAAIMF IFPMLRRWIE NSDWHIFRLL LWWSQPRIYV GRGMHESQIA 670 680 690 700 710 720 LIKYTIFWLL LFCCKFAFSY FLQVKLLVKP TNAIMSIRHV KYKWHEFFPN AEHNYGAVVS 730 740 750 760 770 780 LWLPVILVYF MDTQIWYAIF STICGGVIGA FDRLGEIRTL GMLRSRFQSL PGAFNTYLVP 790 800 810 820 830 840 SDKTRRRGFS LSKRFAEVTA ARRTEAAKFS QLWNEIISSF REEDLISDRE MDLLLVPYTS 850 860 870 880 890 900 DPSLKLIQWP PFLLASKIPI ALDMAAQFRT RDSDLWKRIC ADEYMKCAVI ECYESFKHVL 910 920 930 940 950 960 HTLVIGENEK RIIGIIIKEV ESNISKNSFL SNFRMAPLPA LCSKFVELVG ILKNADPAKR 970 980 990 1000 1010 1020 DTVVLLLQDM LEVVTRDMMQ NENRELVELG HTNKESGRQL FAGTDAKPAI LFPPVATAQW 1030 1040 1050 1060 1070 1080 HEQISRLHLL LTVKESAMDV PTNLEAQRRI AFFTNSLFMD MPRAPRVRNM LSFSVLTPYY 1090 1100 1110 1120 1130 1140 SEETVYSKND LEMENEDGVS VVYYLQKIFP DEWTNFLERL DCKDETSVLE SEENILQLRH 1150 1160 1170 1180 1190 1200 WVSLRGQTLF RTVRGMMYYR RALKLQAFLD MANETEILAG YKAISEPTEE DKKSQRSLYT 1210 1220 1230 1240 1250 1260 QLEAVADLKF TYVATCQNYG NQKRSGDRRA TDILNLMVNN PSLRVAYIDE VEEREGGKVQ 1270 1280 1290 1300 1310 1320 KVFYSVLIKA VDNLDQEIYR IKLPGPAKIG EGKPENQNHA LIFTRGEALQ AIDMNQDHYL 1330 1340 1350 1360 1370 1380 EEALKMRNLL EEFNEDHGVR APTILGFREH IFTGSVSSLA WFMSNQETSF VTIGQRVLAS 1390 1400 1410 1420 1430 1440 PLKVRFHYGH PDVFDRIFHI TRGGISKASR GINLSEDIFA GFNSTLRRGN VTHHEYIQVG 1450 1460 1470 1480 1490 1500 KGRDVGLNQI SLFEAKVACG NGEQTLSRDL YRLGHRFDFF RMMSCYFTTV GFYISSMIVV 1510 1520 1530 1540 1550 1560 LTVYAFLYGR LYLSLSGVEE AIVKFAAAKG DSSLKAAMAS QSVVQLGLLM TLPMVMEIGL 1570 1580 1590 1600 1610 1620 ERGFRTALSD LIIMQLQLAP VFFTFSLGTK VHYYGRTILH GGSKYRATGR GFVVKHEKFA 1630 1640 1650 1660 1670 1680 ENYRMYSRSH FVKGMELMVL LICYRIYGKA AEDSVGYALV MGSTWFLVGS WLFAPFFFNP 1690 1700 1710 1720 1730 1740 SGFEWQKIVD DWDDWNKWIS SRGGIGVPAN KSWESWWEEE QEHLLHSGFF GKFWEIFLSL 1750 1760 1770 1780 1790 1800 RYFIYQYGIV YQLNLTKESR MGKQHSIIVY GLSWLVIVAV MIVLKIVSMG RKKFSADFQL 1810 1820 1830 1840 1850 1860 MFRLLKLFLF IGSVVIVGML FHFLKLTVGD IMQSLLAFLP TGWALLQISQ VARPLMKTVG 1870 1880 1890 1900 1910 1920 MWGSVKALAR GYEYIMGVVI FMPVTVLAWF PFVSEFQTRL LFNQAFSRGL QIQRILAGGK KQK

References

	« Hide 'large scale' referencesShow 'large scale' references »
[1]	"Callose (beta-1,3 glucan) is essential for Arabidopsis pollen wall patterning, but not tube growth." Nishikawa S., Zinkl G.M., Swanson R.J., Maruyama D., Preuss D. BMC Plant Biol. 5:22-22(2005) [PubMed: 16212660] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [MRNA], FUNCTION, DEVELOPMENTAL STAGE, IDENTIFICATION, MUTAGENESIS OF GLY-651, DISRUPTION PHENOTYPE. Strain: cv. Landsberg erecta.
[2]	"Sequence and analysis of chromosome 2 of the plant Arabidopsis thaliana." Lin X., Kaul S., Rounsley S.D., Shea T.P., Benito M.-I., Town C.D., Fujii C.Y., Mason T.M., Bowman C.L., Barnstead M.E., Feldblyum T.V., Buell C.R., Ketchum K.A., Lee J.J., Ronning C.M., Koo H.L., Moffat K.S., Cronin L.A. , Shen M., Pai G., Van Aken S., Umayam L., Tallon L.J., Gill J.E., Adams M.D., Carrera A.J., Creasy T.H., Goodman H.M., Somerville C.R., Copenhaver G.P., Preuss D., Nierman W.C., White O., Eisen J.A., Salzberg S.L., Fraser C.M., Venter J.C. Nature 402:761-768(1999) [PubMed: 10617197] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: cv. Columbia.
[3]	"A cell plate-specific callose synthase and its interaction with phragmoplastin." Hong Z., Delauney A.J., Verma D.P.S. Plant Cell 13:755-768(2001) [PubMed: 11283334] [Abstract] Cited for: GENE FAMILY AND NOMENCLATURE.
[4]	"Callose synthase (CalS5) is required for exine formation during microgametogenesis and for pollen viability in Arabidopsis." Dong X., Hong Z., Sivaramakrishnan M., Mahfouz M., Verma D.P.S. Plant J. 42:315-328(2005) [PubMed: 15842618] [Abstract] Cited for: FUNCTION, DEVELOPMENTAL STAGE, MUTAGENESIS OF GLY-651.
[5]	"Two callose synthases, GSL1 and GSL5, play an essential and redundant role in plant and pollen development and in fertility." Enns L.C., Kanaoka M.M., Torii K.U., Comai L., Okada K., Cleland R.E. Plant Mol. Biol. 58:333-349(2005) [PubMed: 16021399] [Abstract] Cited for: NOMENCLATURE.

Cross-references

Sequence databases
EMBLGenBankDDBJ	AY337762 mRNA. Translation: AAR00322.1. AC006436 Genomic DNA. Translation: AAM15250.1. Sequence problems. AC007063 Genomic DNA. Translation: AAM15369.1. Sequence problems. BK001470 mRNA. Translation: DAA01511.1.
IPI	IPI00516810.
RefSeq	NP_849953.2.
UniGene	At.52728
3D structure databases
ModBase	Search...
Protein family/group databases
CAZy	GT48. Glycosyltransferase Family 48.
Proteomic databases
PRIDE	Q3B724.
Genome annotation databases
GeneID	815852.
GenomeReviews	Gene locus AT2G13680 in contig CT485783_GR.
KEGG	ath:AT2G13680.
NMPDR	fig|3702.1.peg.8414.
Organism-specific databases
TAIR	At2g13680.
Phylogenomic databases
OMA	Q3B724. RRWIENS.
Family and domain databases
InterPro	IPR003440. Glyco_trans_48. [Graphical view]
Pfam	PF02364. Glucan_synthase. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CALS5_ARATH

Accession

Primary (citable) accession number: Q3B724
Secondary accession number(s): Q8S8D4, Q8S8G9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 22, 2005
Last modified:	July 7, 2009
This is version 28 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

PPAP (Plant Proteome Annotation Project)