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Protein

NH(3)-dependent NAD(+) synthetase

Gene

nadE

Organism
Chlorobium luteolum (strain DSM 273 / 2530) (Pelodictyon luteolum)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+.UniRule annotation

Pathwayi: NAD(+) biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route).UniRule annotation
Proteins known to be involved in this subpathway in this organism are:
  1. NH(3)-dependent NAD(+) synthetase (nadE)
This subpathway is part of the pathway NAD(+) biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes NAD(+) from deamido-NAD(+) (ammonia route), the pathway NAD(+) biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Active sitei38 – 381UniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi36 – 438ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Ligase

Keywords - Ligandi

ATP-binding, NAD, Nucleotide-binding

Enzyme and pathway databases

BioCyciPLUT319225:GHDM-578-MONOMER.
UniPathwayiUPA00253; UER00333.

Names & Taxonomyi

Protein namesi
Recommended name:
NH(3)-dependent NAD(+) synthetaseUniRule annotation (EC:6.3.1.5UniRule annotation)
Gene namesi
Name:nadEUniRule annotation
Ordered Locus Names:Plut_0564
OrganismiChlorobium luteolum (strain DSM 273 / 2530) (Pelodictyon luteolum)
Taxonomic identifieri319225 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupPelodictyon
Proteomesi
  • UP000002709 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 277277NH(3)-dependent NAD(+) synthetasePRO_1000077577Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi319225.Plut_0564.

Structurei

3D structure databases

ProteinModelPortaliQ3B5D4.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the NAD synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238069.
KOiK01916.
OMAiCARLRMA.
OrthoDBiEOG6VB6S8.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3B5D4-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQPQDLDLNY GLLEDILKAF LQNEIRKFGF SSVVLGLSGG IDSAVVAELA
60 70 80 90 100
VRALGPEHVL ALKMPYKESS RESLEHADLM IRRLNIKAEE RPVTPMADDF
110 120 130 140 150
FRDVPEGERL RRGNIMARTR MVLLYDVSAR DGSLVAGTSN KTELLLGYGT
160 170 180 190 200
MFGDMASAVN PLGDLYKTQV RGLARHLGIP SILIDKAPSA DLWEGQSDES
210 220 230 240 250
DLGFSYGEVD LLLYMMLELR MEREAILGEG VSASFYDRVR KMVVRNQYKR
260 270
LMPVIAKISG RTPGIDFRYA RDWQEVR
Length:277
Mass (Da):31,121
Last modified:November 22, 2005 - v1
Checksum:i64FC0F8CAB6AF0EE
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000096 Genomic DNA. Translation: ABB23447.1.
RefSeqiWP_011357322.1. NC_007512.1.

Genome annotation databases

EnsemblBacteriaiABB23447; ABB23447; Plut_0564.
KEGGiplt:Plut_0564.
PATRICi21378618. VBIChlLut1287_0587.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000096 Genomic DNA. Translation: ABB23447.1.
RefSeqiWP_011357322.1. NC_007512.1.

3D structure databases

ProteinModelPortaliQ3B5D4.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi319225.Plut_0564.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB23447; ABB23447; Plut_0564.
KEGGiplt:Plut_0564.
PATRICi21378618. VBIChlLut1287_0587.

Phylogenomic databases

eggNOGiENOG4105C4K. Bacteria.
COG0171. LUCA.
HOGENOMiHOG000238069.
KOiK01916.
OMAiCARLRMA.
OrthoDBiEOG6VB6S8.

Enzyme and pathway databases

UniPathwayiUPA00253; UER00333.
BioCyciPLUT319225:GHDM-578-MONOMER.

Family and domain databases

Gene3Di3.40.50.620. 1 hit.
HAMAPiMF_00193. NadE.
InterProiIPR022310. NAD/GMP_synthase.
IPR003694. NAD_synthase.
IPR022926. NH(3)-dep_NAD(+)_synth.
IPR014729. Rossmann-like_a/b/a_fold.
[Graphical view]
PfamiPF02540. NAD_synthase. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00552. nadE. 1 hit.
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Pelodictyon luteolum DSM 273."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: DSM 273 / 2530.

Entry informationi

Entry nameiNADE_CHLL7
AccessioniPrimary (citable) accession number: Q3B5D4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 22, 2005
Last modified: November 11, 2015
This is version 70 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.