Q3B589 (ILVD_PELLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Plut_0609

Organism

Pelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273)) [Complete proteome] [HAMAP]

Taxonomic identifier

319225 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	565 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 565	565	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_1000001025
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3B589 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: B4E7598507D7F621	FASTA	565	59,531
	10 20 30 40 50 60 MRSDTIKTGF EKAPHRSLLK ATGTIHGKSD YQKPFIGICN SFNELIPGHA HLQELGRIAK 70 80 90 100 110 120 EEVRKAGGVP FEFNTIGVCD GIAMGHIGMR YSLASRELIA DSVETVAEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM AALRINIPVI FVSGGPMKAG HTPSGKTVDL ISVFEAVGQR STGEISEEEL 190 200 210 220 230 240 ETIEENACPG CGSCSGMFTA NSMNCLSEAL GFALPGNGTI LAGDPRRNEL VREASRRIID 250 260 270 280 290 300 LVEKNVRPRD ILSRSALLNA FALDFAMGGS TNTILHTLAI ANEAELDFDF SELNGLSAKT 310 320 330 340 350 360 PYICKVSPAT MDVHIEDVDR AGGISAILHE LSKIDGLLDL SVPTVTGKSL GENIKNAEVL 370 380 390 400 410 420 NRKVIRSIED PYSATGGLCV LYGNLAPQGA VIKTGAVSAP MMHHSGPAKV YDSQDDAIAG 430 440 450 460 470 480 IMGGDVHSGD VVVIRYEGPK GGPGMPEMLS PTSAIMGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 IGHVSPEAAV KGPIAALRNG DTVTIDIPGR SITMEVSDEE IADRIAGLPA FVPKIRKGYL 550 560 ARYAEMVTSA NTGAILKTPV SCEPK

References

[1]

"Complete sequence of Pelodictyon luteolum DSM 273." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000096 Genomic DNA. Translation: ABB23492.1.
RefSeq	YP_374535.1. NC_007512.1.
3D structure databases
ProteinModelPortal	Q3B589.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B589.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3744711.
GenomeReviews	Gene locus Plut_0609 in contig CP000096_GR.
KEGG	plt:Plut_0609.
NMPDR	fig|319225.3.peg.242.
PATRIC	21378718. VBIChlLut1287_0636.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q3B589.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	PLUT319225:PLUT_0609-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_PELLD

Accession

Primary (citable) accession number: Q3B589

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 42 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program