Q3B256 (SYE_PELLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Plut_1721

Organism

Pelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273)) [Complete proteome] [HAMAP]

Taxonomic identifier

319225 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	504 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 504	504	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237384
Regions
	Motif	14 – 24	11	"HIGH" region HAMAP MF_00022_B
	Motif	261 – 265	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	264	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3B256 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: CD840631064CD11B	FASTA	504	57,956
	10 20 30 40 50 60 MHMVGKRVRT RFAPSPTGYL HVGGLRTALY NYLFAKKMNG DFIIRIEDTD QSRKVEGAQQ 70 80 90 100 110 120 NLIKTLEWAG LVPDESPVHG GNFGPYLQSE RLELYAGYCR QLLEDGTAYY CFATSEELEE 130 140 150 160 170 180 NRQLQMKQGL QPKYNRKWLP EDMGGSMPRS ESEKMLASGA PYVIRMKVPD YVSVWFEDII 190 200 210 220 230 240 RGPIEFDSAT IDDQVLMKSD GFPTYHFASV IDDHLMEFTH IIRGEEWLPS MPKHLLLYEF 250 260 270 280 290 300 FGWEPPKYAH LPLLLNPDRS KLSKRQGDVA VEDYIQKGYS QEAIINFIAL LGWNEGEGCE 310 320 330 340 350 360 QEVYSMEQLI DRFSLERVGK AGSIFTIDKL NWLEKQYIKN RPAEKIIETI RPLLQEELSS 370 380 390 400 410 420 KETMLDREVI TGDDYLRQVI ELMRERVGFE HEFVTFSSYF FFEPETWEEE AVKKRWTSDT 430 440 450 460 470 480 PALLSEFLPT LEGLPEFTSD AIEAALKAFV EPKGLKAAVL IHPLRILASG VSFGPSLYHM 490 500 LEVLGREAVL RRIRKGMECI TVPA

References

[1]

"Complete sequence of Pelodictyon luteolum DSM 273." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000096 Genomic DNA. Translation: ABB24575.1.
RefSeq	YP_375618.1. NC_007512.1.
3D structure databases
ProteinModelPortal	Q3B256.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B256.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3745189.
GenomeReviews	Gene locus Plut_1721 in contig CP000096_GR.
KEGG	plt:Plut_1721.
NMPDR	fig|319225.3.peg.1725.
PATRIC	21381096. VBIChlLut1287_1805.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	MAHIPLI.
PhylomeDB	Q3B256.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	PLUT319225:PLUT_1721-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_PELLD

Accession

Primary (citable) accession number: Q3B256

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 44 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program