Q3B241 (ISPH_PELLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-hydroxy-3-methylbut-2-enyl diphosphate reductase
EC=1.17.1.2

Gene names

Name:	ispH
Ordered Locus Names:	Plut_1736

Organism

Pelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273)) [Complete proteome] [HAMAP]

Taxonomic identifier

319225 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	320 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Converts 1-hydroxy-2-methyl-2-(E)-butenyl 4-diphosphate into isopentenyl diphosphate (IPP) and dimethylallyl diphosphate (DMAPP) By similarity. HAMAP MF_00191
Catalytic activity	Isopentenyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191 Dimethylallyl diphosphate + NAD(P)+ + H2O = (E)-4-hydroxy-3-methylbut-2-en-1-yl diphosphate + NAD(P)H. HAMAP MF_00191
Cofactor	Binds 1 3Fe-4S cluster By similarity. HAMAP MF_00191
Pathway	Isoprenoid biosynthesis; dimethylallyl diphosphate biosynthesis; dimethylallyl diphosphate from (2E)-4-hydroxy-3-methylbutenyl diphosphate: step 1/1. HAMAP MF_00191 Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 6/6. HAMAP MF_00191
Sequence similarities	Belongs to the IspH family.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	3Fe-4S Iron Iron-sulfur Metal-binding NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	isopentenyl diphosphate biosynthetic process, mevalonate-independent pathway Inferred from electronic annotation. Source: InterPro
Molecular function	3 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW 4-hydroxy-3-methylbut-2-en-1-yl diphosphate reductase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 320	320	4-hydroxy-3-methylbut-2-enyl diphosphate reductase HAMAP MF_00191		PRO_1000021147

Sequences

	Sequence		Length	Mass (Da)
	Q3B241 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: F4F376B4809EC733	FASTA	320	35,152
	10 20 30 40 50 60 MKVNLDRNSS GFCIGVQGTI HVAEEKLRAE IGLYSLGDVV HNEVEVKRLE SLGLVTIDDQ 70 80 90 100 110 120 AFRELRDAPV LIRAHGEPPS TYETARANNL EVTDTTCPVV AKLQRTARQL HLLGYQVIIY 130 140 150 160 170 180 GKPVHPEVIG INGHAANSAV IIKHADLSDP AETAPLDLSR KTALISQTTM DVPGFYQLKE 190 200 210 220 230 240 NLETLFRNAG NPQEGPWTEV RDIDITLGLT GMQPTAPHVY KDTICRQVSS RNSKLHDFAL 250 260 270 280 290 300 SNDCIIFVAG KKSSNGQVLY NICRDANPRS YFIEDTGDLD EGWLKEANGS PVGSVGICGA 310 320 TSTPMWLLEK VARHIESLER

References

[1]

"Complete sequence of Pelodictyon luteolum DSM 273." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000096 Genomic DNA. Translation: ABB24590.1.
RefSeq	YP_375633.1. NC_007512.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B241.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3745204.
GenomeReviews	Gene locus Plut_1736 in contig CP000096_GR.
KEGG	plt:Plut_1736.
NMPDR	fig|319225.3.peg.1740.
PATRIC	21381128. VBIChlLut1287_1821.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0761.
HOGENOM	HBG335228.
OMA	WGAPVYV.
PhylomeDB	Q3B241.
ProtClustDB	PRK01045.
Enzyme and pathway databases
BioCyc	PLUT319225:PLUT_1736-MONOMER.
Family and domain databases
HAMAP	MF_00191. IspH. [Tree]
InterPro	IPR003451. LytB. [Graphical view]
KO	K03527.
Pfam	PF02401. LYTB. 1 hit. [Graphical view]
TIGRFAMs	TIGR00216. IspH_lytB. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPH_PELLD

Accession

Primary (citable) accession number: Q3B241

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 41 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program