Q3B166 (Q3B166_PELLD) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Plut_2073

Organism

Pelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273)) [Complete proteome] [HAMAP]

Taxonomic identifier

319225 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	802 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase SAAS SAAS005733 RuleBase RU000518
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q3B166 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 449D634BC1A4EB97	FASTA	802	89,704
	10 20 30 40 50 60 MASKAAALSA RNRTLIVVES PSKAKTINKY LGDGYTVFAS VGHIKDLPKK EIGLDFDNHY 70 80 90 100 110 120 EPRYEIIPGK EKVVRQLKKL ASEADGVLIA TDPDREGEAI AWHIANEIGS TPKPVFRVMF 130 140 150 160 170 180 NEITKSAILA AIDEPRQIDY RLVRSQQTRQ GLDKIVGYRV SPFLWNVVMR GLSAGRVQSV 190 200 210 220 230 240 ALRLICEREV EINNFEVQEY WTIAADFITE GKETFRARLV KLEGKKPELS NQAEAEAAAD 250 260 270 280 290 300 LVQKGSYTLL DIAPRTQQRK PPLPFTTSLL QQAASNQLGF GSKKTMRLAQ QLYEGIDLGE 310 320 330 340 350 360 EGATGLITYM RTDSIRIGNE AAGQARSFIE HAFGKEYIGW GGAAKSSKNA QDAHEAVRPT 370 380 390 400 410 420 AVGRRPEQLK SYLSADQFKL YDLIWKRFVA AMMAPAKIEQ TRVDVGDPAR DIMFRASGSR 430 440 450 460 470 480 VLFPGFMRVF NDQEELEYEA RKSTKDEGEK EQEVRLPKQL EKDGLLGLGE IDNRQSFTRP 490 500 510 520 530 540 PARFSEATLV KELDNYGIGR PSTYASIFST LQDRRYVELQ KKKIIPTMLG MDVSQILVAN 550 560 570 580 590 600 FPDLFNVDFT AEMEGELDKV AAGEDEYEKV LDSFYRPLET ALSVRKEDPL IPQNRDAALC 610 620 630 640 650 660 EKCGEGHMIV KWTQSGKFLG CSRYPKCRNI KPISTNREKP KDTGILCPSC GEGHMLLRNG 670 680 690 700 710 720 RLGPFLACSS YPKCNTLLNL SKQRHVEPMK IPPVQTDLAC PKCGSPMNLR VGKRGPWLGC 730 740 750 760 770 780 SKFPKCRGRM AWNSLDEGVR AHWEAVMEEH RKAHPSVTLM MLDGTPAPMQ MAVDDIIQMA 790 800 EESGMVEIAV EAPEEAVAEK QD

References

[1]

"Complete sequence of Pelodictyon luteolum DSM 273." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000096 Genomic DNA. Translation: ABB24915.1.
RefSeq	YP_375958.1. NC_007512.1.
3D structure databases
ProteinModelPortal	Q3B166.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B166.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3744202.
GenomeReviews	Gene locus Plut_2073 in contig CP000096_GR.
KEGG	plt:Plut_2073.
NMPDR	fig|319225.3.peg.2053.
PATRIC	21381810. VBIChlLut1287_2153.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q3B166.
ProtClustDB	PRK06599.
Enzyme and pathway databases
BioCyc	PLUT319225:PLUT_2073-MONOMER.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q3B166_PELLD

Accession

Primary (citable) accession number: Q3B166

Entry history

Integrated into UniProtKB/TrEMBL:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	December 14, 2011
This is version 59 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)