Q3B127 (MURD_PELLD) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Plut_2112

Organism

Pelodictyon luteolum (strain DSM 273) (Chlorobium luteolum (strain DSM 273)) [Complete proteome] [HAMAP]

Taxonomic identifier

319225 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Pelodictyon

Protein attributes

Sequence length	460 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 460	460	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257212
Regions
	Nucleotide binding	115 – 121	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3B127 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E346E329DA9EFBF6	FASTA	460	49,476
	10 20 30 40 50 60 MKRADLAGRK VSVIGAGRSG NAAVELLLCH GARVLLSEKG ALDPDTALRF RQRGAEVESE 70 80 90 100 110 120 GHSDRVFDAD FAVVSPGVPP GVPVIRELER RQIPVHSEIE LASWFCRARI AGITGTDGKT 130 140 150 160 170 180 TTATLVQRMA EAVGRLGGYR AYGVGNIGVP FSSKVEEMEE RDIAVVELSS YQLERCSSFR 190 200 210 220 230 240 PEAALITNIT PDHLDRYGGD IMRYADAKYR IAMNLGSSGT LVYNADDPIL RARFSVGGLQ 250 260 270 280 290 300 FSTVPFSTAG PVGGDPSSGI YLEDGWVHAG LRRLIHTSEF QKGSFRGNHN NSNVLGAIGL 310 320 330 340 350 360 ARALRLDEKA VLQALREFPG VEHRQEFVAS KRGSDWINDS KATNVNAMRQ ALEAVPGRIV 370 380 390 400 410 420 LIAGGRDKGN DYSAVATLVR EKADLVVAMG ESRQKVADAF RADVAVVEAA TLEDAVRLAA 430 440 450 460 GGAGTGRTVL FSPGCASFDL FRDFEDRGRS FKAEVGRLEA

References

[1]

"Complete sequence of Pelodictyon luteolum DSM 273." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: DSM 273.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000096 Genomic DNA. Translation: ABB24954.1.
RefSeq	YP_375997.1. NC_007512.1.
3D structure databases
ProteinModelPortal	Q3B127.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3B127.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3745595.
GenomeReviews	Gene locus Plut_2112 in contig CP000096_GR.
KEGG	plt:Plut_2112.
NMPDR	fig|319225.3.peg.1430.
PATRIC	21381892. VBIChlLut1287_2193.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	Q3B127.
Enzyme and pathway databases
BioCyc	PLUT319225:PLUT_2112-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_PELLD

Accession

Primary (citable) accession number: Q3B127

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 47 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program