Reviewed, UniProtKB/Swiss-Prot Q3AWL7 (UCRI_SYNS9)

Names and origin

Protein names

Recommended name:
    Cytochrome b6-f complex iron-sulfur subunit
    EC=1.10.99.1
Alternative name(s):
    Rieske iron-sulfur protein
    Plastohydroquinone:plastocyanin oxidoreductase iron-sulfur protein
      Short name=ISP
      Short name=RISP

Gene names

Name:	petC
Ordered Locus Names:	Syncc9902_1734

Organism

Synechococcus sp. (strain CC9902) [Complete proteome] [HAMAP]

Taxonomic identifier

316279 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	178 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Component of the cytochrome b6-f complex, which mediates electron transfer between photosystem II (PSII) and photosystem I (PSI), cyclic electron flow around PSI, and state transitions By similarity.
Catalytic activity	Plastoquinol-1 + 2 oxidized plastocyanin = plastoquinone + 2 reduced plastocyanin.
Cofactor	Binds 1 2Fe-2S cluster per subunit By similarity.
Subunit structure	The 4 large subunits of the cytochrome b6-f complex are cytochrome b6, subunit IV (17 kDa polypeptide, petD), cytochrome f and the Rieske protein, while the 4 small subunits are petG, petL, petM and petN. The complex functions as a dimer By similarity.
Subcellular location	Cellular thylakoid membrane; Single-pass membrane proteinBy similarity. Note= The transmembrane helix obliquely spans the membrane in one monomer, and its extrinsic C-terminal domain is part of the other monomer By similarity.
Miscellaneous	The Rieske iron-sulfur protein is a high potential 2Fe-2S protein.
Sequence similarities	Contains 1 Rieske domain.

Ontologies

Keywords
Biological process	Electron transport Transport
Cellular component	Membrane Thylakoid
Domain	Transmembrane
Ligand	2Fe-2S Iron Iron-sulfur Metal-binding
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	electron transport chain Inferred from electronic annotation. Source: UniProtKB-KW photosynthesis Inferred from electronic annotation. Source: HAMAP transport Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	integral to membrane Inferred from electronic annotation. Source: UniProtKB-KW thylakoid membrane Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	2 iron, 2 sulfur cluster binding Inferred from electronic annotation. Source: InterPro electron transporter, transferring electrons within cytochrome b6/f complex of photosystem II activity Inferred from electronic annotation. Source: HAMAP iron ion binding Inferred from electronic annotation. Source: UniProtKB-KW plastoquinol-plastocyanin reductase activity Inferred from electronic annotation. Source: HAMAP ubiquinol-cytochrome-c reductase activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 178	178	Cytochrome b6-f complex iron-sulfur subunit		PRO_0000298464
Regions
	Transmembrane	20 – 42	23	Potential
	Domain	65 – 161	97	Rieske
Sites
	Metal binding	107	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	109	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
	Metal binding	125	1	Iron-sulfur (2Fe-2S) By similarity
	Metal binding	128	1	Iron-sulfur (2Fe-2S); via pros nitrogen By similarity
Amino acid modifications
	Disulfide bond	112 ↔ 127		By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AWL7-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E00042B162FEEFD2	FASTA	178	18,737
	10 20 30 40 50 60 MTQIPSSDVP GMGRRQFMNL LTFGSVTGVA LGALYPVARY FIPPKAAGSG GGTTAKDELG 70 80 90 100 110 120 NVVTATGWLS THPEGDRSLV QGLKGDPTYL IVEGADAIGS YGINAICTHL GCVVPWNSGA 130 140 150 160 170 NKFMCPCHGS QYDATGKVVR GPAPLSLALA NVSVEDDNVF VSEWSETDFR TGDKPWWA

References

[1]

"Complete sequence of Synechococcus sp. CC9902." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000097 Genomic DNA. Translation: ABB26691.1.
RefSeq	YP_377735.1.
3D structure databases
SMR	Q3AWL7. Positions 12-177.
ModBase	Search...
Genome annotation databases
GeneID	3742967.
GenomeReviews	Gene locus Syncc9902_1734 in contig CP000097_GR.
KEGG	sye:Syncc9902_1734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3AWL7.
Enzyme and pathway databases
BioCyc	SSP1131-1:SYNCC9902_1734-MON.
Family and domain databases
HAMAP	MF_01335. [Tree]
InterPro	IPR014909. Cyt_b6-F_Fe-S_su. IPR014349. Rieske. IPR005805. Rieske_C. IPR005806. Rieske_reg. IPR006311. Tat. [Graphical view]
Gene3D	G3DSA:2.102.10.10. Rieske_reg. 1 hit.
PANTHER	PTHR10134. Rieske. 1 hit.
Pfam	PF08802. CytB6-F_Fe-S. 1 hit. PF00355. Rieske. 1 hit. [Graphical view]
PRINTS	PR00162. RIESKE.
TIGRFAMs	TIGR01409. TAT_signal_seq. 1 hit.
PROSITE	PS51296. RIESKE. 1 hit. PS51318. TAT. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

UCRI_SYNS9

Accession

Primary (citable) accession number: Q3AWL7

Entry history

Integrated into UniProtKB/Swiss-Prot:	August 21, 2007
Last sequence update:	November 22, 2005
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)