Reviewed, UniProtKB/Swiss-Prot Q3AWF3 (MTNC_SYNS9)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	Syncc9902_1846

Organism

Synechococcus sp. (strain CC9902) [Complete proteome] [HAMAP]

Taxonomic identifier

316279 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	245 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP methionine salvage Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: HAMAP 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: HAMAP acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 245	245	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357422

Sequences

	Sequence		Length	Mass (Da)
	Q3AWF3-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 91F8C9A09016157A	FASTA	245	27,920
	10 20 30 40 50 60 MISHILLDIE GTTCPVTFVT ETLFPYAQLA LKDFLERHKD DPSISQLINN AEDEWMQDQD 70 80 90 100 110 120 KQSATLRHSS EEIQQPKHLK IESYLQLLIA SDKKSTALKD IQGKVWKEGY TTGKITSELF 130 140 150 160 170 180 EDAYEGLKKW HKQGFTLGIY SSGSVEAQRL LYKYTSKGDI ENLFSHWFDT HIGNKKEQRS 190 200 210 220 230 240 YTAIASSMAC KPQNILFISD NSDECDAAKG AGLFTLYSLR EGNPQQEPRD HPIIINLGDV DQWLK

References

[1]

"Complete sequence of Synechococcus sp. CC9902." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000097 Genomic DNA. Translation: ABB26803.1.
RefSeq	YP_377847.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AWF3.
Genome annotation databases
GeneID	3742139.
GenomeReviews	Gene locus Syncc9902_1846 in contig CP000097_GR.
KEGG	sye:Syncc9902_1846.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3AWF3.
OMA	TTDLNFI.
Enzyme and pathway databases
BioCyc	SSP1131-1:SYNCC9902_1846-MON.
Family and domain databases
HAMAP	MF_01681. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SYNS9

Accession

Primary (citable) accession number: Q3AWF3

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 30 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)