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Protein

tRNA (guanine-N(7)-)-methyltransferase

Gene

trmB

Organism
Synechococcus sp. (strain CC9902)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA.UniRule annotation

Catalytic activityi

S-adenosyl-L-methionine + guanine(46) in tRNA = S-adenosyl-L-homocysteine + N(7)-methylguanine(46) in tRNA.UniRule annotation

Pathway: N(7)-methylguanine-tRNA biosynthesis

This protein is involved in the pathway N(7)-methylguanine-tRNA biosynthesis, which is part of tRNA modification.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway N(7)-methylguanine-tRNA biosynthesis and in tRNA modification.

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei35 – 351S-adenosyl-L-methionineUniRule annotation
Binding sitei60 – 601S-adenosyl-L-methionineUniRule annotation
Binding sitei87 – 871S-adenosyl-L-methionineUniRule annotation
Active sitei113 – 1131By similarity
Binding sitei113 – 1131S-adenosyl-L-methionineUniRule annotation
Binding sitei117 – 1171SubstrateUniRule annotation
Binding sitei149 – 1491SubstrateUniRule annotation

GO - Molecular functioni

Complete GO annotation...

Keywords - Molecular functioni

Methyltransferase, Transferase

Keywords - Biological processi

tRNA processing

Keywords - Ligandi

S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSP316279:GJCI-2099-MONOMER.
UniPathwayiUPA00989.

Names & Taxonomyi

Protein namesi
Recommended name:
tRNA (guanine-N(7)-)-methyltransferaseUniRule annotation (EC:2.1.1.33UniRule annotation)
Alternative name(s):
tRNA (guanine(46)-N(7))-methyltransferaseUniRule annotation
tRNA(m7G46)-methyltransferaseUniRule annotation
Gene namesi
Name:trmBUniRule annotation
Ordered Locus Names:Syncc9902_2064
OrganismiSynechococcus sp. (strain CC9902)
Taxonomic identifieri316279 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaOscillatoriophycideaeChroococcalesSynechococcus
ProteomesiUP000002712 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 236236tRNA (guanine-N(7)-)-methyltransferasePRO_0000288244Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi316279.Syncc9902_2064.

Structurei

3D structure databases

ProteinModelPortaliQ3AW35.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the class I-like SAM-binding methyltransferase superfamily. TrmB family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000237936.
KOiK03439.
OMAiVNPLSRF.
OrthoDBiEOG6K6VBC.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3AW35-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRQHVNPLSR SFQLPLRLPP PSELFTHPEH PIHLDIGCAR GRCILDLADR
60 70 80 90 100
HPNWNHIGVE IRRPLVIPAD REALESPHGN VRVLFCNANI SLEGWMETLP
110 120 130 140 150
KDRLQRVSIQ FPDPWFKRRH RKRRVLQPRL LMAIASALQP GKDFFIQSDV
160 170 180 190 200
REVIDPMVAL TELSGCFDRP ENDAHPWRRT NPLTVPTERE RYVMDQQLPV
210 220 230
YRVLFRRNHT HQPPIEEFEQ HWQEIDNPGN APTPDA
Length:236
Mass (Da):27,647
Last modified:November 22, 2005 - v1
Checksum:i2BBBE85EE3C3BDE2
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000097 Genomic DNA. Translation: ABB27022.1.
RefSeqiWP_011360809.1. NC_007513.1.
YP_378065.1. NC_007513.1.

Genome annotation databases

EnsemblBacteriaiABB27022; ABB27022; Syncc9902_2064.
KEGGisye:Syncc9902_2064.
PATRICi23801693. VBISynSp76179_2278.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000097 Genomic DNA. Translation: ABB27022.1.
RefSeqiWP_011360809.1. NC_007513.1.
YP_378065.1. NC_007513.1.

3D structure databases

ProteinModelPortaliQ3AW35.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi316279.Syncc9902_2064.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB27022; ABB27022; Syncc9902_2064.
KEGGisye:Syncc9902_2064.
PATRICi23801693. VBISynSp76179_2278.

Phylogenomic databases

eggNOGiCOG0220.
HOGENOMiHOG000237936.
KOiK03439.
OMAiVNPLSRF.
OrthoDBiEOG6K6VBC.

Enzyme and pathway databases

UniPathwayiUPA00989.
BioCyciSSP316279:GJCI-2099-MONOMER.

Family and domain databases

Gene3Di3.40.50.150. 1 hit.
HAMAPiMF_01057. tRNA_methyltr_TrmB.
InterProiIPR029063. SAM-dependent_MTases.
IPR003358. tRNA_(Gua-N-7)_MeTrfase_Trmb.
[Graphical view]
PfamiPF02390. Methyltransf_4. 1 hit.
[Graphical view]
SUPFAMiSSF53335. SSF53335. 1 hit.
TIGRFAMsiTIGR00091. TIGR00091. 1 hit.
PROSITEiPS51625. SAM_MT_TRMB. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Synechococcus sp. CC9902."
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Martinez M., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CC9902.

Entry informationi

Entry nameiTRMB_SYNS9
AccessioniPrimary (citable) accession number: Q3AW35
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 29, 2007
Last sequence update: November 22, 2005
Last modified: May 27, 2015
This is version 59 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.