Q3ATL6 (KATG_CHLCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Catalase-peroxidase
Short name=CP
EC=1.11.1.21

Alternative name(s):
Peroxidase/catalase

Gene names

Name:	katG
Ordered Locus Names:	Cag_0386

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	733 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Bifunctional enzyme with both catalase and broad-spectrum peroxidase activity By similarity. HAMAP MF_01961
Catalytic activity	Donor + H2O2 = oxidized donor + 2 H2O. HAMAP MF_01961 2 H2O2 = O2 + 2 H2O. HAMAP MF_01961
Cofactor	Binds 1 heme B (iron-protoporphyrin IX) group per dimer By similarity.
Subunit structure	Homodimer or homotetramer By similarity. HAMAP MF_01961
Post-translational modification	The covalent Trp-Tyr-Met adduct is important for the catalase, but not the peroxidase activity of the enzyme By similarity. HAMAP MF_01961
Sequence similarities	Belongs to the peroxidase family. Peroxidase/catalase subfamily.

Ontologies

Keywords
Biological process	Hydrogen peroxide
Ligand	Heme Iron Metal-binding
Molecular function	Oxidoreductase Peroxidase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	hydrogen peroxide catabolic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	catalase activity Inferred from electronic annotation. Source: InterPro heme binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 733	733	Catalase-peroxidase HAMAP MF_01961		PRO_0000354757
Sites
	Active site	97	1	Proton acceptor By similarity
	Metal binding	260	1	Iron (heme axial ligand) By similarity
	Site	93	1	Transition state stabilizer By similarity
Amino acid modifications
	Cross-link	96 ↔ 219		Tryptophyl-tyrosyl-methioninium (Trp-Tyr) (with M-245) By similarity
	Cross-link	219 ↔ 245		Tryptophyl-tyrosyl-methioninium (Tyr-Met) (with W-96) By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ATL6 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 353611606058E8DD	FASTA	733	81,677
	10 20 30 40 50 60 MNEERKCPIT GATHKPSAEK GRSNHDWWPN QLNLKILHQH SALSNPMDKD FNYAEEFKKL 70 80 90 100 110 120 DLAAIKQDLY ALMTDSQEWW PADYGHYGPL FIRMAWHSAG TYRTSDGRGG AGTGSQRFAP 130 140 150 160 170 180 LNSWPDNANL DKARRLLWPI KQKYGRQISW ADLMILTGNC ALESMGLKTF GFAGGREDIW 190 200 210 220 230 240 EPEEDIYWGT EGEWLADKRY SGERELEKPL AAVQMGLIYV NPEGPNGKPD PLAAAKDIRE 250 260 270 280 290 300 TFARMAMNDE ETVALIAGGH TFGKTHGAGD ASQVGPEPEA AGIEEQGLGW KNQYGTGKGK 310 320 330 340 350 360 DTITSGLEVI WTTTPTKWSN NFFWNLFGYE WELTKSPAGA YQWTPKYGVG ANTVPDAHDP 370 380 390 400 410 420 SKRHAPAMMT TDLALRFDPD YEKIARRYYE NPDQFADAFA RAWFKLTHRD MGPRSRYRGA 430 440 450 460 470 480 EVPVEELIWQ DTIPALDHEL IGADEIAALK ATILASELSI AQLISTAWAS AATFRNSDKR 490 500 510 520 530 540 GGANGARLRL APQKDWEVNQ PDELQKVLQV LETIQTEFNA SRNDGKKVSL ADLIVLGGCA 550 560 570 580 590 600 AIEAAAEKAG YKVTVPFTPG RMDATQEETD AHSFAVLEPV ADGFRNYLKA KYSFSVEEML 610 620 630 640 650 660 IDKAQLLTLT APEMTVLIGG MRVLNTNAGH TTHGVFTKRP ETLSNDFFVN LLDMGTVWKA 670 680 690 700 710 720 TSEASDIFEG RNRSTGELQW TATRVDLVFG SNSQLRALVE VYGCKDSQEK FLNDFIAAWN 730 KVMNLDRFDL SGL

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CaD3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB27659.1.
RefSeq	YP_378702.1. NC_007514.1.
3D structure databases
HSSP	HSSP built from PDB template 2CCA based on UniProtKB Q50555.
ProteinModelPortal	Q3ATL6.
SMR	Q3ATL6. Positions 21-731.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ATL6.
Protein family/group databases
PeroxiBase	2669. CchCP01.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3747554.
GenomeReviews	Gene locus Cag_0386 in contig CP000108_GR.
KEGG	cch:Cag_0386.
PATRIC	21368355. VBIChlChl46571_0404.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0376.
HOGENOM	HBG285610.
OMA	KRHAPSM.
PhylomeDB	Q3ATL6.
ProtClustDB	PRK15061.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_0386-MONOMER.
Family and domain databases
HAMAP	MF_01961. Catal-peroxid. [Tree]
InterPro	IPR000763. Catalase_peroxidase. IPR010255. Haem_peroxidase. IPR002016. Haem_peroxidase_pln/fun/bac. IPR019794. Peroxidases_AS. IPR019793. Peroxidases_heam-ligand_BS. [Graphical view]
KO	K03782.
Pfam	PF00141. peroxidase. 2 hits. [Graphical view]
PRINTS	PR00460. BPEROXIDASE. PR00458. PEROXIDASE.
SUPFAM	SSF48113. Peroxidase_super. 2 hits.
TIGRFAMs	TIGR00198. Cat_per_HPI. 1 hit.
PROSITE	PS00435. PEROXIDASE_1. 1 hit. PS00436. PEROXIDASE_2. 1 hit. PS50873. PEROXIDASE_4. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

KATG_CHLCH

Accession

Primary (citable) accession number: Q3ATL6

Entry history

Integrated into UniProtKB/Swiss-Prot:	November 25, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program