Q3ATL0 (SYE_CHLCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Glutamate--tRNA ligase
EC=6.1.1.17

Alternative name(s):
Glutamyl-tRNA synthetase
Short name=GluRS

Gene names

Name:	gltX
Ordered Locus Names:	Cag_0392

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	502 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction: glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) By similarity. HAMAP MF_00022_B
Catalytic activity	ATP + L-glutamate + tRNA(Glu) = AMP + diphosphate + L-glutamyl-tRNA(Glu). HAMAP MF_00022_B
Subunit structure	Monomer By similarity. HAMAP MF_00022_B
Subcellular location	Cytoplasm HAMAP MF_00022_B.
Sequence similarities	Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glutamyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW glutamate-tRNA ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 502	502	Glutamate--tRNA ligase HAMAP MF_00022_B		PRO_0000237353
Regions
	Motif	12 – 22	11	"HIGH" region HAMAP MF_00022_B
	Motif	259 – 263	5	"KMSKS" region HAMAP MF_00022_B
Sites
	Binding site	262	1	ATP By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ATL0 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 710022D59566A909	FASTA	502	57,687
	10 20 30 40 50 60 MVGQRVRTRF APSPTGYLHV GGLRTALYNF LFAKKMKGDF IIRIEDTDQS RKVEGAQQNL 70 80 90 100 110 120 IKTLEWAGII ADESPQHGGS CGPYIQSERL DIYAKHCKQL LEDHHAYYCF ATPEELEENR 130 140 150 160 170 180 QLQLKQGLQP KYNRKWLPEE MGGTMPESLI RQKLEAGEPY VIRMKVPDYI SVWFEDIIRG 190 200 210 220 230 240 PIEFDSATID DQVLMKSDGF PTYHFASVID DHLMEISHII RGEEWLSSMP KHLLLYEFFG 250 260 270 280 290 300 WEAPKVAHLP LLLNPDRSKL SKRQGDVAVE DYIRKGYSSE AIVNFVALLG WNEGEGCEQE 310 320 330 340 350 360 VYSLQELTDK FSLERVGKAG SIFTLDKLKW LEKQYIKNRS VEKLLSVVKP LLLEELEKKP 370 380 390 400 410 420 SIMAREQITS DSYLSSVIEL MRERVGFEHE FVTFSTYFYF EPESYDEESV KKRWQPNTNE 430 440 450 460 470 480 LLADFIPQLE ALSDFTAENI EAALKAFVEP KGMKNAVLIH PLRIVTSGVG FGPSLYHMLE 490 500 VLGKETVLRR IRKGLECITM PA

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CaD3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB27665.1.
RefSeq	YP_378708.1. NC_007514.1.
3D structure databases
ProteinModelPortal	Q3ATL0.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ATL0.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3747560.
GenomeReviews	Gene locus Cag_0392 in contig CP000108_GR.
KEGG	cch:Cag_0392.
PATRIC	21368367. VBIChlChl46571_0410.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0008.
HOGENOM	HBG628189.
OMA	ANFNDES.
PhylomeDB	Q3ATL0.
ProtClustDB	PRK01406.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_0392-MONOMER.
Family and domain databases
HAMAP	MF_00022_B. Glu_tRNA_synth_B. [Tree]
InterPro	IPR008925. aa-tRNA-synth_I_codon-bd. IPR020751. aa-tRNA-synth_I_codon-bd_sub2. IPR001412. aa-tRNA-synth_I_CS. IPR004527. Glu-tRNA-synth_Ib_bac/mito. IPR000924. Glu/Gln-tRNA-synth_Ib. IPR020061. Glu/Gln-tRNA-synth_Ib_a-bdl. IPR020058. Glu/Gln-tRNA-synth_Ib_cat-dom. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:1.10.1160.10. Glu/Gln-tRNA-synth_Ic_a-bdl. 1 hit. G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 2 hits. G3DSA:1.10.10.350. tRNA_synt_bd. 1 hit.
KO	K01885.
PANTHER	PTHR10119. Glu_tRNA-synt_1c. 1 hit. PTHR10119:SF1. PTHR10119:SF1. 1 hit.
Pfam	PF00749. tRNA-synt_1c. 1 hit. [Graphical view]
PRINTS	PR00987. TRNASYNTHGLU.
SUPFAM	SSF48163. tRNA-synt_bind. 1 hit.
TIGRFAMs	TIGR00464. GltX_bact. 1 hit.
PROSITE	PS00178. AA_TRNA_LIGASE_I. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYE_CHLCH

Accession

Primary (citable) accession number: Q3ATL0

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 30, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program