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Protein

Probable endonuclease 4

Gene

nfo

Organism
Chlorobium chlorochromatii (strain CaD3)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Endonuclease IV plays a role in DNA repair. It cleaves phosphodiester bonds at apurinic or apyrimidinic sites (AP sites) to produce new 5'-ends that are base-free deoxyribose 5-phosphate residues. It preferentially attacks modified AP sites created by bleomycin and neocarzinostatin.UniRule annotation

Catalytic activityi

Endonucleolytic cleavage to 5'-phosphooligonucleotide end-products.UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 3 Zn2+ ions.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Zinc 1UniRule annotation
Metal bindingi109 – 1091Zinc 1UniRule annotation
Metal bindingi145 – 1451Zinc 1UniRule annotation
Metal bindingi145 – 1451Zinc 2UniRule annotation
Metal bindingi179 – 1791Zinc 2UniRule annotation
Metal bindingi182 – 1821Zinc 3UniRule annotation
Metal bindingi216 – 2161Zinc 2UniRule annotation
Metal bindingi229 – 2291Zinc 3UniRule annotation
Metal bindingi231 – 2311Zinc 3UniRule annotation
Metal bindingi261 – 2611Zinc 2UniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Endonuclease, Hydrolase, Nuclease

Keywords - Biological processi

DNA damage, DNA repair

Keywords - Ligandi

Metal-binding, Zinc

Enzyme and pathway databases

BioCyciCCHL340177:GHBW-543-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Probable endonuclease 4UniRule annotation (EC:3.1.21.2UniRule annotation)
Alternative name(s):
Endodeoxyribonuclease IVUniRule annotation
Endonuclease IVUniRule annotation
Gene namesi
Name:nfoUniRule annotation
Ordered Locus Names:Cag_0528
OrganismiChlorobium chlorochromatii (strain CaD3)
Taxonomic identifieri340177 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
Proteomesi
  • UP000002708 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 282282Probable endonuclease 4PRO_1000011298Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi340177.Cag_0528.

Structurei

3D structure databases

ProteinModelPortaliQ3AT74.
SMRiQ3AT74. Positions 1-279.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the AP endonuclease 2 family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3AT74-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKRVGAHVSA SGGVEQAPLN ATAIGAKAFA LFTKNQRQWK APKLSKATIE
60 70 80 90 100
AFQKACADGG FQPQHILPHD SYLINLGSPD PEKLERARSA FIDEMQRVAD
110 120 130 140 150
LGLQLLNFHP GSHLKEISEE ASLLLIAESI NMALEATNGV TAVIENTAGQ
160 170 180 190 200
GTNLGYRFEQ IAFLIDRIED KSRVGVCLDT CHLFASGYDL SSTEAIETTF
210 220 230 240 250
NEFDSTVGLH YLRGMHLNDA MQPLGSRVDR HASLGKGTIG MAAFTFIMNH
260 270 280
PACEEIPLIL ETPNPDIWSE EIALLYSLQQ VD
Length:282
Mass (Da):30,794
Last modified:November 22, 2005 - v1
Checksum:iD0E45349B9272054
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000108 Genomic DNA. Translation: ABB27801.1.
RefSeqiWP_011361574.1. NC_007514.1.

Genome annotation databases

EnsemblBacteriaiABB27801; ABB27801; Cag_0528.
KEGGicch:Cag_0528.
PATRICi21368661. VBIChlChl46571_0550.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000108 Genomic DNA. Translation: ABB27801.1.
RefSeqiWP_011361574.1. NC_007514.1.

3D structure databases

ProteinModelPortaliQ3AT74.
SMRiQ3AT74. Positions 1-279.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340177.Cag_0528.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB27801; ABB27801; Cag_0528.
KEGGicch:Cag_0528.
PATRICi21368661. VBIChlChl46571_0550.

Phylogenomic databases

eggNOGiENOG4105EFU. Bacteria.
COG0648. LUCA.
HOGENOMiHOG000224893.
KOiK01151.
OMAiMKYVGAH.
OrthoDBiEOG6Z0QCM.

Enzyme and pathway databases

BioCyciCCHL340177:GHBW-543-MONOMER.

Family and domain databases

Gene3Di3.20.20.150. 1 hit.
HAMAPiMF_00152. Nfo.
InterProiIPR001719. AP_endonuc_2.
IPR018246. AP_endonuc_F2_Zn_BS.
IPR013022. Xyl_isomerase-like_TIM-brl.
[Graphical view]
PANTHERiPTHR21445. PTHR21445. 1 hit.
PfamiPF01261. AP_endonuc_2. 1 hit.
[Graphical view]
SMARTiSM00518. AP2Ec. 1 hit.
[Graphical view]
SUPFAMiSSF51658. SSF51658. 1 hit.
TIGRFAMsiTIGR00587. nfo. 1 hit.
PROSITEiPS00729. AP_NUCLEASE_F2_1. 1 hit.
PS00730. AP_NUCLEASE_F2_2. 1 hit.
PS00731. AP_NUCLEASE_F2_3. 1 hit.
PS51432. AP_NUCLEASE_F2_4. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Chlorobium chlorochromatii CaD3."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CaD3.

Entry informationi

Entry nameiEND4_CHLCH
AccessioniPrimary (citable) accession number: Q3AT74
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: November 11, 2015
This is version 74 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.