Reviewed, UniProtKB/Swiss-Prot Q3AQC2 (SAHH_CHLCH)

Names and origin

Protein names

Recommended name:
    Adenosylhomocysteinase
    EC=3.3.1.1
Alternative name(s):
    S-adenosyl-L-homocysteine hydrolase
      Short name=AdoHcyase

Gene names

Name:	ahcY
Ordered Locus Names:	Cag_1548

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	471 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	S-adenosyl-L-homocysteine + H2O = L-homocysteine + adenosine. HAMAP MF_00563
Cofactor	Binds 1 NAD per subunit By similarity. HAMAP MF_00563
Pathway	Amino-acid biosynthesis; L-homocysteine biosynthesis; L-homocysteine from S-adenosyl-L-homocysteine: step 1/1. HAMAP MF_00563
Subcellular location	Cytoplasm By similarity HAMAP MF_00563.
Sequence similarities	Belongs to the adenosylhomocysteinase family.

Ontologies

Keywords
Biological process	One-carbon metabolism
Cellular component	Cytoplasm
Ligand	NAD
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	one-carbon metabolic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	adenosylhomocysteinase activity Inferred from electronic annotation. Source: HAMAP binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 471	471	Adenosylhomocysteinase HAMAP MF_00563		PRO_1000024722
Regions
	Region	223 – 391	169	NAD binding By similarity
Sites
	Binding site	60	1	Substrate By similarity
	Binding site	135	1	Substrate By similarity
	Binding site	196	1	Substrate By similarity
	Binding site	226	1	Substrate By similarity
	Binding site	230	1	Substrate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AQC2-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 04F2691F7CF61C72	FASTA	471	52,247
	10 20 30 40 50 60 MSTVAEALAY KVADISLAEW GRKEIEIAEK EMPGLMALRK KYAGQKPLKG ARIAGSLHMT 70 80 90 100 110 120 IQTAVLIETL VDLGANVRWA SCNIFSTQDH AAAAIAQAGV PVFAWKGETL DEYWWCTRQI 130 140 150 160 170 180 LEFDGGLGPN LIVDDGGDAT LMIILGHKIE NDPKMLDKTP GNAEEKALYQ QLREVFAEDN 190 200 210 220 230 240 QRWHKVAADM KGVSEETTTG VHRLYQMMEK GELLFPAINV NDSVTKSKFD NLYGCRESLA 250 260 270 280 290 300 DGIKRATDVM IAGKVTVVLG YGDVGKGCAH SMRSYGARVI VTEIDPICAL QAAMEGFEVA 310 320 330 340 350 360 TMEDAVHEGN IFVTTTGNKD VITLEHMKQM RDEAIVCNIG HFDNEIQVDK LNNDPTVKII 370 380 390 400 410 420 NIKPQVDKYI FPNGNCMYLL AEGRLVNLGC ATGHPSFVMS NSFTNQTLAQ IELWQKEYAV 430 440 450 460 470 DVYRLPKALD EEVARLHLEQ IGVRLTRLTP EQAEYIGVSA DGPYKPEHYR Y

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB28803.1.
RefSeq	YP_379846.1.
3D structure databases
HSSP	HSSP built from PDB template 1K0U based on UniProtKB P10760.
SMR	Q3AQC2. Positions 10-471.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AQC2.
Genome annotation databases
GeneID	3746548.
GenomeReviews	Gene locus Cag_1548 in contig CP000108_GR.
KEGG	cch:Cag_1548.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0499.
HOGENOM	HBG352029.
OMA	VITHDHM.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_1548-MONOMER.
Family and domain databases
HAMAP	MF_00563_B. AdoHcyase_B. [Tree]
InterPro	IPR015878. Ado_hCys_hydrolase_NAD-bd. IPR016040. NAD(P)-bd_dom. IPR000043. S-Ado-L-homoCys_hydrolase. IPR020082. S-Ado-L-homoCys_hydrolase_CS. [Graphical view]
PANTHER	PTHR23420. Ad_hcy_hydrolase. 1 hit.
Pfam	PF05221. AdoHcyase. 1 hit. PF00670. AdoHcyase_NAD. 1 hit. [Graphical view]
PIRSF	PIRSF001109. Ad_hcy_hydrolase. 1 hit.
TIGRFAMs	TIGR00936. ahcY. 1 hit.
PROSITE	PS00738. ADOHCYASE_1. 1 hit. PS00739. ADOHCYASE_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SAHH_CHLCH

Accession

Primary (citable) accession number: Q3AQC2

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	February 9, 2010
This is version 38 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)