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Protein

Acetyl-coenzyme A synthetase

Gene

acsA

Organism
Chlorobium chlorochromatii (strain CaD3)
Status
Unreviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the conversion of acetate into acetyl-CoA (AcCoA), an essential intermediate at the junction of anabolic and catabolic pathways. AcsA undergoes a two-step reaction. In the first half reaction, AcsA combines acetate with ATP to form acetyl-adenylate (AcAMP) intermediate. In the second half reaction, it can then transfer the acetyl group from AcAMP to the sulfhydryl group of CoA, forming the product AcCoA.UniRule annotation

Catalytic activityi

ATP + acetate + CoA = AMP + diphosphate + acetyl-CoA.UniRule annotation

Cofactori

Mg2+UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei331 – 3311Coenzyme AUniRule annotation
Binding sitei355 – 3551Coenzyme AUniRule annotation
Binding sitei520 – 5201ATPUniRule annotation
Binding sitei535 – 5351ATPUniRule annotation
Binding sitei543 – 5431Coenzyme A; via carbonyl oxygenUniRule annotation
Binding sitei546 – 5461ATPUniRule annotation
Metal bindingi557 – 5571Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi559 – 5591Magnesium; via carbonyl oxygenUniRule annotation
Metal bindingi562 – 5621Magnesium; via carbonyl oxygenUniRule annotation

Regions

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Nucleotide bindingi407 – 4093ATPUniRule annotation
Nucleotide bindingi431 – 4366ATPUniRule annotation

GO - Molecular functioni

  1. acetate-CoA ligase activity Source: UniProtKB-HAMAP
  2. AMP binding Source: InterPro
  3. ATP binding Source: UniProtKB-KW
  4. metal ion binding Source: UniProtKB-KW

GO - Biological processi

  1. acetyl-CoA biosynthetic process from acetate Source: InterPro
Complete GO annotation...

Keywords - Molecular functioni

LigaseUniRule annotationImported

Keywords - Ligandi

ATP-bindingUniRule annotation, MagnesiumUniRule annotation, Metal-bindingUniRule annotation, Nucleotide-binding

Enzyme and pathway databases

BioCyciCCHL340177:GHBW-1701-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Acetyl-coenzyme A synthetaseUniRule annotation (EC:6.2.1.1UniRule annotation)
Short name:
AcCoA synthetaseUniRule annotation
Short name:
AcsUniRule annotation
Alternative name(s):
Acetate--CoA ligaseUniRule annotation
Acyl-activating enzymeUniRule annotation
Gene namesi
Name:acsAUniRule annotation
Ordered Locus Names:Cag_1685Imported
OrganismiChlorobium chlorochromatii (strain CaD3)Imported
Taxonomic identifieri340177 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000002708: Chromosome

PTM / Processingi

Amino acid modifications

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Modified residuei629 – 6291N6-acetyllysineUniRule annotation

Post-translational modificationi

Acetylated. Deacetylation by the SIR2-homolog deacetylase activates the enzyme.UniRule annotation

Keywords - PTMi

AcetylationUniRule annotation

Interactioni

Protein-protein interaction databases

STRINGi340177.Cag_1685.

Structurei

3D structure databases

ProteinModelPortaliQ3APY8.
SMRiQ3APY8. Positions 31-664.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Regioni213 – 2164Coenzyme A bindingUniRule annotation

Sequence similaritiesi

Belongs to the ATP-dependent AMP-binding enzyme family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiSHRCLTI.
OrthoDBiEOG68WR2H.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3APY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MATETPSSSQ QEAAANPAED SISSVLTEKR KFPPPASFSE QAHLSTMEQY
60 70 80 90 100
EKLYADAAAD PEGYWAGIAE QFHWFKKWDS VLEWNSPYAK WFNGGKTNIC
110 120 130 140 150
YNALDVHVKS WRKNKAAVIW EGEQGDQRIL TYGELHRQVC KFANVLKIAG
160 170 180 190 200
IKPGDRIAIY MGMVPELMIA VLACARVGAV HNVIFAGFSA HAITERVNDS
210 220 230 240 250
RAKMVICADG TRRRGSTINL KNIVDEAIVN TPSVRNVIVL KTTGETIKMH
260 270 280 290 300
DGMDHWWHDL MGLAVDESEA VELDAEHPLF VLYTSGSTGK PKGILHTTAG
310 320 330 340 350
YMVHAASSFR YVFDIKDEDI YFCTADIGWI TGHSYMVYGP LLNGATLLMY
360 370 380 390 400
EGAPNYPQWD RFWDIINRHK VTILYTAPTA IRAFIRAGNE WVTKHNLNSL
410 420 430 440 450
RLLGTVGEPI NPEAWMWYHK VVGQEKCPIV DTWWQTETGG IMVSPMPGAT
460 470 480 490 500
PTKPGTATRP LPGIMVDVVH KDGTPCGANE GGYLVIKKPW PSMLRTIYGD
510 520 530 540 550
NERYEKTYWS EFKDMYFTGD GARKDDDGYI WIMGRVDDVV NVSGHRLGTS
560 570 580 590 600
EVESALVSHE AVAEAAVVSR PDDIKGNSLV AFVTLKDEYE GDMKLRESLR
610 620 630 640 650
NHVAREIGPI AKPDEIRWAK ALPKTRSGKI MRRLLRELAT SNEIKGDVTT
660
LEDFGVLENL RDQENE
Length:666
Mass (Da):74,565
Last modified:November 22, 2005 - v1
Checksum:iBAC348AF52226EDF
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000108 Genomic DNA. Translation: ABB28937.1.
RefSeqiYP_379980.1. NC_007514.1.

Genome annotation databases

EnsemblBacteriaiABB28937; ABB28937; Cag_1685.
GeneIDi3746375.
KEGGicch:Cag_1685.
PATRICi21371113. VBIChlChl46571_1751.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000108 Genomic DNA. Translation: ABB28937.1.
RefSeqiYP_379980.1. NC_007514.1.

3D structure databases

ProteinModelPortaliQ3APY8.
SMRiQ3APY8. Positions 31-664.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi340177.Cag_1685.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB28937; ABB28937; Cag_1685.
GeneIDi3746375.
KEGGicch:Cag_1685.
PATRICi21371113. VBIChlChl46571_1751.

Phylogenomic databases

eggNOGiCOG0365.
HOGENOMiHOG000229981.
KOiK01895.
OMAiSHRCLTI.
OrthoDBiEOG68WR2H.

Enzyme and pathway databases

BioCyciCCHL340177:GHBW-1701-MONOMER.

Family and domain databases

HAMAPiMF_01123. Ac_CoA_synth.
InterProiIPR011904. Ac_CoA_lig.
IPR025110. AMP-bd_C.
IPR020845. AMP-binding_CS.
IPR000873. AMP-dep_Synth/Lig.
[Graphical view]
PfamiPF00501. AMP-binding. 1 hit.
PF13193. AMP-binding_C. 1 hit.
[Graphical view]
TIGRFAMsiTIGR02188. Ac_CoA_lig_AcsA. 1 hit.
PROSITEiPS00455. AMP_BINDING. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. "Complete sequence of Chlorobium chlorochromatii CaD3."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CaD3Imported.

Entry informationi

Entry nameiQ3APY8_CHLCH
AccessioniPrimary (citable) accession number: Q3APY8
Entry historyi
Integrated into UniProtKB/TrEMBL: November 22, 2005
Last sequence update: November 22, 2005
Last modified: January 7, 2015
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusiUnreviewed (UniProtKB/TrEMBL)

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteomeImported

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.