Reviewed, UniProtKB/Swiss-Prot Q3APW3 (SYFA_CHLCH)

Names and origin

Protein names

Recommended name:
    Phenylalanyl-tRNA synthetase alpha chain
    EC=6.1.1.20
Alternative name(s):
    Phenylalanine--tRNA ligase alpha chain
      Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	Cag_1711

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	344 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity.
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm. HAMAP MF_00281
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: HAMAP phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 344	344	Phenylalanyl-tRNA synthetase alpha chain HAMAP MF_00281		PRO_0000231974
Sites
	Metal binding	257	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3APW3-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 3B356C51BE5EAF0C	FASTA	344	38,566
	10 20 30 40 50 60 MEERILQAKQ EILEAPLTTL AELESFRLHF TVRKGLVAAL FGELKNVASA DKPRIGQLLN 70 80 90 100 110 120 ELKQTAESRV SDAEAHLAAA QNSKQQPSLL DLTLPGRRSF CGSEHPVQKV LGDMKSIFNA 130 140 150 160 170 180 MGFTIATGPE LEVGNYNFDM LNFAPDHPAR DMQDTFFVRT GSGNTPDVLL RTHTSPVQIR 190 200 210 220 230 240 VMLDEKPPIR VICPGKVYRN EAISARSYCV FHQLEGLYID KNVSFADLKA TIYSFAQQMF 250 260 270 280 290 300 GSDVKLRFRP SFFPFTEPSA EVDVTCYLCG GKGCRVCKKS GWLEILGCGM VHPNVLRNCG 310 320 330 340 IDPEEWSGYA FGMGVDRTVL LRYKIDDIRL LFENDVRMLQ QFKA

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000108 Genomic DNA. Translation: ABB28962.1.
RefSeq	YP_380005.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3746977.
GenomeReviews	Gene locus Cag_1711 in contig CP000108_GR.
KEGG	cch:Cag_1711.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3APW3.
OMA	Q3APW3. FRASYFP.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_1711-MON.
Family and domain databases
HAMAP	MF_00281. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II_cons-reg. IPR002319. Phe-tRNA-synth_IIc-rel. IPR004529. Phe-tRNA-synth_IIc_asu. IPR018157. Phe-tRNA-synth_IIc_C. [Graphical view]
PANTHER	PTHR11538. tRNA-synt_2d. 1 hit.
Pfam	PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
TIGRFAMs	TIGR00468. pheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_CHLCH

Accession

Primary (citable) accession number: Q3APW3

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 23 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)