ID   Q3APV4_CHLCH            Unreviewed;       265 AA.
AC   Q3APV4;
DT   22-NOV-2005, integrated into UniProtKB/TrEMBL.
DT   22-NOV-2005, sequence version 1.
DT   27-MAR-2024, entry version 93.
DE   RecName: Full=Putative 2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase {ECO:0000256|HAMAP-Rule:MF_01660};
DE            Short=SHCHC synthase {ECO:0000256|HAMAP-Rule:MF_01660};
DE            EC=4.2.99.20 {ECO:0000256|HAMAP-Rule:MF_01660};
GN   Name=menH {ECO:0000256|HAMAP-Rule:MF_01660};
GN   OrderedLocusNames=Cag_1720 {ECO:0000313|EMBL:ABB28971.1};
OS   Chlorobium chlorochromatii (strain CaD3).
OC   Bacteria; Chlorobiota; Chlorobiia; Chlorobiales; Chlorobiaceae;
OC   Chlorobium/Pelodictyon group; Chlorobium.
OX   NCBI_TaxID=340177 {ECO:0000313|EMBL:ABB28971.1};
RN   [1] {ECO:0000313|EMBL:ABB28971.1}
RP   NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
RC   STRAIN=CaD3 {ECO:0000313|EMBL:ABB28971.1};
RG   US DOE Joint Genome Institute;
RA   Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T.,
RA   Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F.,
RA   Land M., Kyrpides N., Ivanova N., Richardson P.;
RT   "Complete sequence of Chlorobium chlorochromatii CaD3.";
RL   Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases.
CC   -!- FUNCTION: Catalyzes a proton abstraction reaction that results in 2,5-
CC       elimination of pyruvate from 2-succinyl-5-enolpyruvyl-6-hydroxy-3-
CC       cyclohexene-1-carboxylate (SEPHCHC) and the formation of 2-succinyl-6-
CC       hydroxy-2,4-cyclohexadiene-1-carboxylate (SHCHC). {ECO:0000256|HAMAP-
CC       Rule:MF_01660}.
CC   -!- CATALYTIC ACTIVITY:
CC       Reaction=5-enolpyruvoyl-6-hydroxy-2-succinyl-cyclohex-3-ene-1-
CC         carboxylate = (1R,6R)-6-hydroxy-2-succinyl-cyclohexa-2,4-diene-1-
CC         carboxylate + pyruvate; Xref=Rhea:RHEA:25597, ChEBI:CHEBI:15361,
CC         ChEBI:CHEBI:58689, ChEBI:CHEBI:58818; EC=4.2.99.20;
CC         Evidence={ECO:0000256|HAMAP-Rule:MF_01660};
CC   -!- PATHWAY: Quinol/quinone metabolism; 1,4-dihydroxy-2-naphthoate
CC       biosynthesis; 1,4-dihydroxy-2-naphthoate from chorismate: step 3/7.
CC       {ECO:0000256|HAMAP-Rule:MF_01660}.
CC   -!- PATHWAY: Quinol/quinone metabolism; menaquinone biosynthesis.
CC       {ECO:0000256|HAMAP-Rule:MF_01660}.
CC   -!- SUBUNIT: Monomer. {ECO:0000256|HAMAP-Rule:MF_01660}.
CC   -!- SIMILARITY: Belongs to the AB hydrolase superfamily. MenH family.
CC       {ECO:0000256|HAMAP-Rule:MF_01660}.
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DR   EMBL; CP000108; ABB28971.1; -; Genomic_DNA.
DR   AlphaFoldDB; Q3APV4; -.
DR   STRING; 340177.Cag_1720; -.
DR   ESTHER; chlch-q3apv4; MenH_SHCHC.
DR   KEGG; cch:Cag_1720; -.
DR   eggNOG; COG2267; Bacteria.
DR   HOGENOM; CLU_020336_50_4_10; -.
DR   OrthoDB; 252464at2; -.
DR   UniPathway; UPA00079; -.
DR   UniPathway; UPA01057; UER00900.
DR   GO; GO:0070205; F:2-succinyl-6-hydroxy-2,4-cyclohexadiene-1-carboxylate synthase activity; IEA:UniProtKB-UniRule.
DR   GO; GO:0004177; F:aminopeptidase activity; IEA:UniProtKB-KW.
DR   GO; GO:0009234; P:menaquinone biosynthetic process; IEA:UniProtKB-UniRule.
DR   Gene3D; 3.40.50.1820; alpha/beta hydrolase; 1.
DR   HAMAP; MF_01660; MenH; 1.
DR   InterPro; IPR029058; AB_hydrolase.
DR   InterPro; IPR000073; AB_hydrolase_1.
DR   InterPro; IPR022485; SHCHC_synthase_MenH.
DR   NCBIfam; TIGR03695; menH_SHCHC; 1.
DR   PANTHER; PTHR42916; 2-SUCCINYL-5-ENOLPYRUVYL-6-HYDROXY-3-CYCLOHEXENE-1-CARBOXYLATE SYNTHASE; 1.
DR   PANTHER; PTHR42916:SF1; PROTEIN PHYLLO, CHLOROPLASTIC; 1.
DR   Pfam; PF00561; Abhydrolase_1; 1.
DR   PRINTS; PR00111; ABHYDROLASE.
DR   SUPFAM; SSF53474; alpha/beta-Hydrolases; 1.
PE   3: Inferred from homology;
KW   Aminopeptidase {ECO:0000313|EMBL:ABB28971.1};
KW   Hydrolase {ECO:0000313|EMBL:ABB28971.1};
KW   Lyase {ECO:0000256|ARBA:ARBA00023239, ECO:0000256|HAMAP-Rule:MF_01660};
KW   Menaquinone biosynthesis {ECO:0000256|ARBA:ARBA00022428, ECO:0000256|HAMAP-
KW   Rule:MF_01660}; Protease {ECO:0000313|EMBL:ABB28971.1}.
FT   DOMAIN          20..251
FT                   /note="AB hydrolase-1"
FT                   /evidence="ECO:0000259|Pfam:PF00561"
SQ   SEQUENCE   265 AA;  29422 MW;  D36006E75EA886E4 CRC64;
     MIMQPPLHIE IIGNKALPKI VFLHGFLGSG RDWLPLAEML TSHYCCVLVD LPGHGSATLS
     ASDEHHAYFT ATVEALATVI QPISPEPCRL VGYSMGGRIA LALMLTHPEL FHQAVIVSAS
     PGLPTEEERA KRRAGDEGIA RKIERNFPDF LEAWYQQPLF STLKNHPLFQ EIERKRAINN
     SESLAAALRL LGTGQQPSFW DALSKCAVPT LFIAGEKDER YVAIARQMVK LAPHATLSIV
     PNCGHTLHIE NKESFVEQLH TFFNQ
//