Reviewed, UniProtKB/Swiss-Prot Q3APU0 (DAPA_CHLCH)

Names and origin

Protein names

Recommended name:
    Dihydrodipicolinate synthase
      Short name=DHDPS
    EC=4.2.1.52

Gene names

Name:	dapA
Ordered Locus Names:	Cag_1734

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	296 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Catalytic activity	L-aspartate 4-semialdehyde + pyruvate = dihydrodipicolinate + 2 H2O. HAMAP MF_00418
Pathway	Amino-acid biosynthesis; L-lysine biosynthesis via DAP pathway; (S)-tetrahydrodipicolinate from L-aspartate: step 3/4. HAMAP MF_00418
Subunit structure	Homotetramer By similarity.
Subcellular location	Cytoplasm By similarity.
Sequence similarities	Belongs to the DHDPS family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Diaminopimelate biosynthesis Lysine biosynthesis
Cellular component	Cytoplasm
Ligand	Schiff base
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	diaminopimelate biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	dihydrodipicolinate synthase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 296	296	Dihydrodipicolinate synthase HAMAP MF_00418		PRO_1000050173
Regions
	Region	52 – 53	2	Pyruvate binding By similarity
Sites
	Active site	166	1	Schiff-base intermediate with substrate By similarity
	Binding site	110	1	Pyruvate By similarity
	Site	137	1	Involved in proton transfer during cleavage By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3APU0-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: B8C8DEE665213796	FASTA	296	31,732
	10 20 30 40 50 60 MPTPYLSGSA VALVTPFKSD SSIDFEAIAR LTEFHVAAGT NIIIPCGTTG ESPTLAEEEQ 70 80 90 100 110 120 VAIIKTVVEA AQGKLMVAAG AGTNNTHHAV ELAKNAEKAG AAAILSVAPY YNKPSQEGFY 130 140 150 160 170 180 QHYRHIAEAV AVPIIIYNVP GRTGCNVAAS TILRLARDFD NVLAVKEASE NFTQISELLE 190 200 210 220 230 240 ERPSNFAVLT GEDSLILPFM AMGGNGVISV AANEIPAQIR QLVESAGSGD LVTARTLYSR 250 260 270 280 290 YRKLLKLNFI ESNPVPVKYA LARMGMIEEN YRLPLVPLSA ESKRAMDEEL TLLGLV

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000108 Genomic DNA. Translation: ABB28985.1.
RefSeq	YP_380028.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3APU0.
Genome annotation databases
GeneID	3746517.
GenomeReviews	Gene locus Cag_1734 in contig CP000108_GR.
KEGG	cch:Cag_1734.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3APU0.
OMA	QKGLIEH.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_1734-MON.
Family and domain databases
HAMAP	MF_00418. [Tree]
InterPro	IPR013785. Aldolase_TIM. IPR005263. DapA_synth. IPR002220. DHDPS. [Graphical view]
Gene3D	G3DSA:3.20.20.70. Aldolase_TIM. 1 hit.
PANTHER	PTHR12128. DHDPS. 1 hit.
Pfam	PF00701. DHDPS. 1 hit. [Graphical view]
PRINTS	PR00146. DHPICSNTHASE.
ProDom	PD001859. DHDPS. 1 hit. [Graphical view] [Entries sharing at least one domain]
TIGRFAMs	TIGR00674. dapA. 1 hit.
PROSITE	PS00665. DHDPS_1. False negative. PS00666. DHDPS_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DAPA_CHLCH

Accession

Primary (citable) accession number: Q3APU0

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 31 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)