Reviewed, UniProtKB/Swiss-Prot Q3APC4 (LEU3_CHLCH)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	Cag_1901

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	352 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 352	352	3-isopropylmalate dehydrogenase		PRO_0000250111
Regions
	Nucleotide binding	76 – 89	14	NAD By similarity
	Nucleotide binding	276 – 288	13	NAD By similarity
Sites
	Metal binding	219	1	Magnesium or manganese By similarity
	Metal binding	243	1	Magnesium or manganese By similarity
	Metal binding	247	1	Magnesium or manganese By similarity
	Binding site	96	1	Substrate By similarity
	Binding site	106	1	Substrate By similarity
	Binding site	134	1	Substrate By similarity
	Binding site	219	1	Substrate By similarity
	Site	141	1	Important for catalysis By similarity
	Site	187	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3APC4-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 4CEC7D44CA5275FB	FASTA	352	38,185
	10 20 30 40 50 60 MNYKIVSIPG DGIGTEVVAG AVAVLRQLEK KYGFTVEIEE HLFGGASYDV HGEMLTDATL 70 80 90 100 110 120 EACKNCDAVL LGAVGGPKWE NLPHEHKPEA ALLKIRKELG LFANLRPAKV YDALVDASSL 130 140 150 160 170 180 KADVVRGTDF VVFRELTGGI YFGQPRGYDE NKGWNTMVYE KYEVERIARL AFEAARQRQG 190 200 210 220 230 240 RVMSIDKANV LEVSQLWRNV VHAVHADYQD VELSDMYVDN AAMQIVRNPK QFDVIVTGNL 250 260 270 280 290 300 FGDILSDISG MITGSLGMLP SASIGSKHAL YEPIHGSAPD IAGQNKANPI ATIASVAMMF 310 320 330 340 350 EHSFKRTDIA RDIEQAIEAA LATGVRTADI AAAGDTAVST TAMTEAIISQ LK

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000108 Genomic DNA. Translation: ABB29151.1.
RefSeq	YP_380194.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3747646.
GenomeReviews	Gene locus Cag_1901 in contig CP000108_GR.
KEGG	cch:Cag_1901.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3APC4.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_1901-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CHLCH

Accession

Primary (citable) accession number: Q3APC4

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 22, 2005
Last modified:	November 4, 2008
This is version 27 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)