Q3APB9 (ILVD_CHLCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Dihydroxy-acid dehydratase
Short name=DAD
EC=4.2.1.9

Gene names

Name:	ilvD
Ordered Locus Names:	Cag_1906

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	559 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	2,3-dihydroxy-3-methylbutanoate = 3-methyl-2-oxobutanoate + H2O. HAMAP MF_00012
Cofactor	Binds 1 4Fe-4S cluster Potential.
Pathway	Amino-acid biosynthesis; L-isoleucine biosynthesis; L-isoleucine from 2-oxobutanoate: step 3/4. HAMAP MF_00012 Amino-acid biosynthesis; L-valine biosynthesis; L-valine from pyruvate: step 3/4. HAMAP MF_00012
Sequence similarities	Belongs to the IlvD/Edd family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis
Ligand	4Fe-4S Iron Iron-sulfur Metal-binding
Molecular function	Lyase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	branched chain family amino acid biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	4 iron, 4 sulfur cluster binding Inferred from electronic annotation. Source: UniProtKB-KW dihydroxy-acid dehydratase activity Inferred from electronic annotation. Source: EC metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 559	559	Dihydroxy-acid dehydratase HAMAP MF_00012		PRO_0000225382
Sites
	Metal binding	121	1	Iron-sulfur (4Fe-4S) Potential
	Metal binding	194	1	Iron-sulfur (4Fe-4S) Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3APB9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 0683F9E2FAB7B5ED	FASTA	559	58,691
	10 20 30 40 50 60 MRSDTIKSGF EKAPHRSLLK ATGAIRSSSD YRKPFIGICN SYNELIPGHT HLQELGRIAK 70 80 90 100 110 120 EAVREAGGVP FEFNTIGVCD GIAMGHIGMR YSLASRELIA DSVETVAEAH RLDGLVCIPN 130 140 150 160 170 180 CDKITPGMMM AALRINIPVI FVSGGPMKAG HTPEGKTVDL ISVFEAVGQC SNGSITEGEL 190 200 210 220 230 240 QNIEEHACPG CGSCSGMFTA NSMNCLSEAL GFALPGNGTI VAEDPRRLEL VKAASRRIVD 250 260 270 280 290 300 LVENNVRPRD ILTRQALLNA FALDFAMGGS TNTILHTLAI ANEAGLSFDF SELNALSAKT 310 320 330 340 350 360 PYICQVSPAT MAVHIEDVDR AGGISAILKE LSSIDGLLDL SAITVTGKTL GENIANAEVL 370 380 390 400 410 420 DRSVIRSISD PYSATGGLAV LYGNLAPQGA VVKTGAVSPQ MMQHSGPAKV YNAQDDAIKG 430 440 450 460 470 480 IMEGDVKAGD VVVIRYEGPK GGPGMPEMLS PTSAIMGRGL GDSVALITDG RFSGGSRGAC 490 500 510 520 530 540 IGHVSPEAAE RGPIAALQNG DIITIDIPAR TMSVALSEST IKERLAQLPP FEPKIKRGYL 550 ARYAQLVTSA NTGAILGHL

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CaD3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB29156.1.
RefSeq	YP_380199.1. NC_007514.1.
3D structure databases
ProteinModelPortal	Q3APB9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3APB9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3747651.
GenomeReviews	Gene locus Cag_1906 in contig CP000108_GR.
KEGG	cch:Cag_1906.
PATRIC	21371584. VBIChlChl46571_1985.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0129.
HOGENOM	HBG671001.
OMA	SQDCRWP.
PhylomeDB	Q3APB9.
ProtClustDB	PRK00911.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_1906-MONOMER.
Family and domain databases
HAMAP	MF_00012. IlvD. [Tree]
InterPro	IPR015928. Aconitase/3IPM_dehydase_swvl. IPR004404. DihydroxyA_deHydtase. IPR000581. DiOHA_6PGluconate_deHydtase. IPR020558. DiOHA_6PGluconate_deHydtase_CS. [Graphical view]
KO	K01687.
PANTHER	PTHR21000. ILVD_EDD_family. 1 hit.
Pfam	PF00920. ILVD_EDD. 1 hit. [Graphical view]
SUPFAM	SSF52016. Aconitase/3IPM_dehydase_swvl. 1 hit.
TIGRFAMs	TIGR00110. IlvD. 1 hit.
PROSITE	PS00886. ILVD_EDD_1. 1 hit. PS00887. ILVD_EDD_2. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

ILVD_CHLCH

Accession

Primary (citable) accession number: Q3APB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program