Q3ANX7 (Q3ANX7_CHLCH) Unreviewed, UniProtKB/TrEMBL

Names and origin

Protein names

Recommended name:
DNA topoisomerase RuleBase RU000518
EC=5.99.1.2 RuleBase RU000518

Gene names

Ordered Locus Names:

Cag_0075

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	798 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Releases the supercoiling and torsional tension of DNA introduced during the DNA replication and transcription by transiently cleaving and rejoining one strand of the DNA duplex. Introduces a single-strand break via transesterification at a target site in duplex DNA. The scissile phosphodiester is attacked by the catalytic tyrosine of the enzyme, resulting in the formation of a DNA-(5'-phosphotyrosyl)-enzyme intermediate and the expulsion of a 3'-OH DNA strand. The free DNA strand than undergoes passage around the unbroken strand thus removing DNA supercoils. Finally, in the religation step, the DNA 3'-OH attacks the covalent intermediate to expel the active-site tyrosine and restore the DNA phosphodiester backbone By similarity. SAAS SAAS000380
Catalytic activity	ATP-independent breakage of single-stranded DNA, followed by passage and rejoining. RuleBase RU000518 SAAS SAAS005733
Sequence similarities	Belongs to the prokaryotic type I/III topoisomerase family. RuleBase RU004206 Contains 1 Toprim domain. SAAS SAAS023406

Ontologies

Keywords
Ligand	ATP-binding SAAS SAAS005733 DNA-binding SAAS SAAS005733 RuleBase RU000518 Nucleotide-binding
Molecular function	Isomerase Topoisomerase RuleBase RU000518 SAAS SAAS005733
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	DNA topological change Inferred from electronic annotation. Source: InterPro
Cellular component	chromosome Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW DNA topoisomerase type I activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequences

	Sequence		Length	Mass (Da)
	Q3ANX7 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E7DCDC308590CE83	FASTA	798	88,630
	10 20 30 40 50 60 MASSVAALSA KNKTLIVVES PSKAKTINKY LGSNYTVFAS VGHIKDLPKK EIGLDFEHNY 70 80 90 100 110 120 SPRYEIIPGK EKVVKQLKKL ATEASNILIA TDPDREGEAI AWHIANEIEH AKAPVARVLF 130 140 150 160 170 180 NEVTKKAILE AIEKPRHIDL RLVHSQQTRQ GLDKIVGYKI SPFLWKVVLR GLSAGRVQSV 190 200 210 220 230 240 ALRLICEREE EIERFVIQEY WTIAADFLTA NKESFRARLV RLDGDKPEIT NVEQAEAIAA 250 260 270 280 290 300 IAKKGNYSVR EITPRIQQRK QPLPFTTSLL QQAASNQLGF GAQRTMRTAQ QLYEGIELGA 310 320 330 340 350 360 EGAMGLITYM RTDSTRISPE AVGEARNYIE RNFGKDYVGA GSSGKPGKNA QDAHEAIRPT 370 380 390 400 410 420 SLLKTPEQVK PYLSADQFKL YELIWKRFLA AMMAPAKIEQ TKVDVEEQSG KFLFRANGSR 430 440 450 460 470 480 VLFPGFMRVY DDQQELAYEA QTSTKEEVEN EMVVKLPEKL AVNDPLGLGA LEQKQSFTRP 490 500 510 520 530 540 PARYSEASLV KDLDHFGIGR PSTYASIFST LQDRRYVALE KRKIMPTDLG RDVAKILVAN 550 560 570 580 590 600 FPELFNVGFT AFMEDELDKV ASGDDAYEKV LDSFYKPLTS ALALRSATPL IPQNNEAETC 610 620 630 640 650 660 DKCGTGKMIL KWTASGKFLG CSNYPKCKNI RTISSNREKP ASTGVHCPSC EDGEMVLRKG 670 680 690 700 710 720 RLGPFLACSN YPKCNTLLNL NKQRHIEPPK TPPVVTDMAC PKCGAPLYLR SGKRGLWLGC 730 740 750 760 770 780 SKFPKCRGRL AWTALEPAAQ ERWERVMAAH QKAHPPVTLK MVDGSTVSMT SSIDDIIMKA 790 DAAGLIAPAM DLVPEAEG

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CaD3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB27354.1.
RefSeq	YP_378397.1. NC_007514.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ANX7.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3746409.
GenomeReviews	Gene locus Cag_0075 in contig CP000108_GR.
KEGG	cch:Cag_0075.
PATRIC	21367681. VBIChlChl46571_0079.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0550.
HOGENOM	HBG512935.
OMA	EEYWSID.
PhylomeDB	Q3ANX7.
ProtClustDB	PRK06599.
Family and domain databases
InterPro	IPR000380. Topo_IA. IPR003601. Topo_IA_2. IPR023406. Topo_IA_AS. IPR013497. Topo_IA_cen. IPR013824. Topo_IA_cen_sub1. IPR013825. Topo_IA_cen_sub2. IPR023405. Topo_IA_core_domain. IPR003602. Topo_IA_DNA-bd. IPR013498. Topo_IA_Znf. IPR005733. TopoI_bac-type. IPR006171. Toprim_domain. [Graphical view]
Gene3D	G3DSA:1.10.460.10. Topo_IA_cen_sub1. 2 hits. G3DSA:2.70.20.10. Topo_IA_cen_sub2. 2 hits.
KO	K03168.
PANTHER	PTHR11390. Topo_IA. 1 hit.
Pfam	PF01131. Topoisom_bac. 1 hit. PF01751. Toprim. 1 hit. PF01396. zf-C4_Topoisom. 3 hits. [Graphical view]
PRINTS	PR00417. PRTPISMRASEI.
SMART	SM00437. TOP1Ac. 1 hit. SM00436. TOP1Bc. 1 hit. SM00493. TOPRIM. 1 hit. [Graphical view]
SUPFAM	SSF56712. Topo_IA_core. 1 hit.
TIGRFAMs	TIGR01051. TopA_bact. 1 hit.
PROSITE	PS00396. TOPOISOMERASE_I_PROK. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

Q3ANX7_CHLCH

Accession

Primary (citable) accession number: Q3ANX7

Entry history

Integrated into UniProtKB/TrEMBL:	November 22, 2005
Last sequence update:	November 22, 2005
Last modified:	December 14, 2011
This is version 58 of the entry and version 1 of the sequence. [Complete history]

Entry status

Unreviewed (UniProtKB/TrEMBL)