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Q3ANX4

- BIOB_CHLCH

UniProt

Q3ANX4 - BIOB_CHLCH

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Protein
Biotin synthase
Gene
bioB, Cag_0078
Organism
Chlorobium chlorochromatii (strain CaD3)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism By similarity.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine.UniRule annotation

Cofactori

Binds 1 4Fe-4S cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine By similarity.
Binds 1 2Fe-2S cluster. The cluster is coordinated with 3 cysteines and 1 arginine By similarity.

Pathwayi

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Metal bindingi69 – 691Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi73 – 731Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi76 – 761Iron-sulfur 1 (4Fe-4S-S-AdoMet) By similarity
Metal bindingi148 – 1481Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi209 – 2091Iron-sulfur 2 (2Fe-2S) By similarity
Metal bindingi279 – 2791Iron-sulfur 2 (2Fe-2S) By similarity

GO - Molecular functioni

  1. 2 iron, 2 sulfur cluster binding Source: UniProtKB-KW
  2. 4 iron, 4 sulfur cluster binding Source: UniProtKB-KW
  3. biotin synthase activity Source: UniProtKB-HAMAP
  4. iron ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. biotin biosynthetic process Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Transferase

Keywords - Biological processi

Biotin biosynthesis

Keywords - Ligandi

2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciCCHL340177:GHBW-80-MONOMER.
UniPathwayiUPA00078; UER00162.

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthase (EC:2.8.1.6)
Gene namesi
Name:bioB
Ordered Locus Names:Cag_0078
OrganismiChlorobium chlorochromatii (strain CaD3)
Taxonomic identifieri340177 [NCBI]
Taxonomic lineageiBacteriaChlorobiChlorobiaChlorobialesChlorobiaceaeChlorobium/Pelodictyon groupChlorobium
ProteomesiUP000002708: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 330330Biotin synthaseUniRule annotation
PRO_0000381297Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi340177.Cag_0078.

Structurei

3D structure databases

ProteinModelPortaliQ3ANX4.

Family & Domainsi

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0502.
HOGENOMiHOG000239958.
KOiK01012.
OMAiCKEDCIF.
OrthoDBiEOG622PMP.

Family and domain databases

Gene3Di3.20.20.70. 1 hit.
HAMAPiMF_01694. BioB.
InterProiIPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view]
PfamiPF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view]
PIRSFiPIRSF001619. Biotin_synth. 1 hit.
SMARTiSM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view]
TIGRFAMsiTIGR00433. bioB. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3ANX4-1 [UniParc]FASTAAdd to Basket

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MLHPAIESAY RVLATGEPLT HGEALQLANL SGSDLLDLAS LAHKVQLRYA    50
GGVESFHACS IMNAKSGVCG ENCRFCAQSK HNNAAIEVYD LVSDDSVLIE 100
ARQAVANGVS HFGIVTSGYG YQRITPEFER LLGMIDRLHS ELPSLNVCAS 150
FGVLGEEPVA ALAAHGIAHY NINLQVEPNR YNDLIATTHS AEERIATIHR 200
LRQHNIAVCC GGIIGVGESV EERIGLLIAL QALDLAVIPL NVLVPIAGTP 250
LEKSEPLSAA EVLKTFAICR LLHPRTIIKF AAGRETMMKD FQGLLMLAGV 300
NGFLTGGYLT TRGRDVADDQ RFAAQLASFA 330
Length:330
Mass (Da):35,421
Last modified:November 22, 2005 - v1
Checksum:iDBAD7D5AD0293DB3
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000108 Genomic DNA. Translation: ABB27357.1.
RefSeqiWP_011361132.1. NC_007514.1.
YP_378400.1. NC_007514.1.

Genome annotation databases

EnsemblBacteriaiABB27357; ABB27357; Cag_0078.
GeneIDi3746412.
KEGGicch:Cag_0078.
PATRICi21367687. VBIChlChl46571_0082.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000108 Genomic DNA. Translation: ABB27357.1 .
RefSeqi WP_011361132.1. NC_007514.1.
YP_378400.1. NC_007514.1.

3D structure databases

ProteinModelPortali Q3ANX4.
ModBasei Search...
MobiDBi Search...

Protein-protein interaction databases

STRINGi 340177.Cag_0078.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB27357 ; ABB27357 ; Cag_0078 .
GeneIDi 3746412.
KEGGi cch:Cag_0078.
PATRICi 21367687. VBIChlChl46571_0082.

Phylogenomic databases

eggNOGi COG0502.
HOGENOMi HOG000239958.
KOi K01012.
OMAi CKEDCIF.
OrthoDBi EOG622PMP.

Enzyme and pathway databases

UniPathwayi UPA00078 ; UER00162 .
BioCyci CCHL340177:GHBW-80-MONOMER.

Family and domain databases

Gene3Di 3.20.20.70. 1 hit.
HAMAPi MF_01694. BioB.
InterProi IPR013785. Aldolase_TIM.
IPR010722. BATS_dom.
IPR002684. Biotin_synth/BioAB.
IPR024177. Biotin_synthase.
IPR006638. Elp3/MiaB/NifB.
IPR007197. rSAM.
[Graphical view ]
Pfami PF06968. BATS. 1 hit.
PF04055. Radical_SAM. 1 hit.
[Graphical view ]
PIRSFi PIRSF001619. Biotin_synth. 1 hit.
SMARTi SM00876. BATS. 1 hit.
SM00729. Elp3. 1 hit.
[Graphical view ]
TIGRFAMsi TIGR00433. bioB. 1 hit.
ProtoNeti Search...

Publicationsi

  1. "Complete sequence of Chlorobium chlorochromatii CaD3."
    US DOE Joint Genome Institute
    Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P.
    Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases
    Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: CaD3.

Entry informationi

Entry nameiBIOB_CHLCH
AccessioniPrimary (citable) accession number: Q3ANX4
Entry historyi
Integrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 22, 2005
Last modified: September 3, 2014
This is version 63 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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