Q3ANV5 (MURD_CHLCH) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylmuramoylalanine--D-glutamate ligase
EC=6.3.2.9

Alternative name(s):
D-glutamic acid-adding enzyme
UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Gene names

Name:	murD
Ordered Locus Names:	Cag_0053

Organism

Chlorobium chlorochromatii (strain CaD3) [Complete proteome] [HAMAP]

Taxonomic identifier

340177 [NCBI]

Taxonomic lineage

Bacteria › Chlorobi › Chlorobia › Chlorobiales › Chlorobiaceae › Chlorobium/Pelodictyon group › Chlorobium

Protein attributes

Sequence length	464 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) By similarity. HAMAP MF_00639
Catalytic activity	ATP + UDP-N-acetylmuramoyl-L-alanine + glutamate = ADP + phosphate + UDP-N-acetylmuramoyl-L-alanyl-D-glutamate. HAMAP MF_00639
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00639
Subcellular location	Cytoplasm By similarity HAMAP MF_00639.
Sequence similarities	Belongs to the MurCDEF family.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW UDP-N-acetylmuramoylalanine-D-glutamate ligase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 464	464	UDP-N-acetylmuramoylalanine--D-glutamate ligase HAMAP MF_00639		PRO_0000257178
Regions
	Nucleotide binding	112 – 118	7	ATP Potential

Sequences

	Sequence		Length	Mass (Da)
	Q3ANV5 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E6597FD84C94433D	FASTA	464	49,969
	10 20 30 40 50 60 MDVAGKKVTV LGGGKSGVAA ALLLQQLGAT VLLSEHGALS SEAMQRLQAA HIAYEANGHS 70 80 90 100 110 120 EQIYSADFCV LSPGIPPTAP VVQQMEAHSI PLYSEIEVAS CFCKARMVGI TGTDGKTTTS 130 140 150 160 170 180 TLIHTLCEAD GKRHGYRSYS VGNSGIPFSS MVLAMQPNDV AVIELSSYQL ERSISFHPQV 190 200 210 220 230 240 SLITNITPDH LDRYGQNMQR YAEAKYRIFM NQQAGDTFIY NQDDSMLQAA FGASQIAVPC 250 260 270 280 290 300 RSVAFGLEPL TNVQLDKRRV LVNGNMVVVR QNDGALQPIV AVDEVLNRAF RGKHNLSNVL 310 320 330 340 350 360 AAVAVGEALG IGSEVMRQAL TAFGGVEHRQ ELVATIDGVE WINDSKATNV NAMRQALEAV 370 380 390 400 410 420 PAPMILIAGG RDKGNNYATV SHLIERKVCL LIATGESREK LASFFKGKVP VIAVPTIDEA 430 440 450 460 VAIAHQQAKA GESVLFSPAC ASFDMFNNFE ERGAFFKQCV RQVL

References

[1]

"Complete sequence of Chlorobium chlorochromatii CaD3." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Bryant D., Schmutz J., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (AUG-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CaD3.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000108 Genomic DNA. Translation: ABB27332.1.
RefSeq	YP_378375.1. NC_007514.1.
3D structure databases
ProteinModelPortal	Q3ANV5.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ANV5.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3747252.
GenomeReviews	Gene locus Cag_0053 in contig CP000108_GR.
KEGG	cch:Cag_0053.
PATRIC	21367631. VBIChlChl46571_0055.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG0771.
HOGENOM	HBG750024.
OMA	ACASWDM.
PhylomeDB	Q3ANV5.
ProtClustDB	CLSK2310674.
Enzyme and pathway databases
BioCyc	CCHL340177:CAG_0053-MONOMER.
Family and domain databases
HAMAP	MF_00639. MurD. [Tree]
InterPro	IPR004101. Mur_ligase_C. IPR013221. Mur_ligase_cen. IPR016040. NAD(P)-bd_dom. IPR005762. UDP-N-AcMur-Glu_ligase. [Graphical view]
Gene3D	G3DSA:3.90.190.20. Mur_ligase_C. 1 hit. G3DSA:3.40.1190.10. Mur_ligase_cen. 1 hit. G3DSA:3.40.50.720. NAD(P)-bd. 1 hit.
KO	K01925.
PANTHER	PTHR23135:SF2. PTHR23135:SF2. 1 hit.
Pfam	PF02875. Mur_ligase_C. 1 hit. PF08245. Mur_ligase_M. 1 hit. [Graphical view]
SUPFAM	SSF53244. Mur_ligase_C. 1 hit. SSF53623. Mur_ligase_cen. 1 hit.
TIGRFAMs	TIGR01087. MurD. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MURD_CHLCH

Accession

Primary (citable) accession number: Q3ANV5

Entry history

Integrated into UniProtKB/Swiss-Prot:	October 31, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 50 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program