Reviewed, UniProtKB/Swiss-Prot Q3AMB9 (MTNC_SYNSC)

Names and origin

Protein names

Recommended name:
    Enolase-phosphatase E1
    EC=3.1.3.77
Alternative name(s):
    2,3-diketo-5-methylthio-1-phosphopentane phosphatase

Gene names

Name:	mtnC
Ordered Locus Names:	Syncc9605_0489

Organism

Synechococcus sp. (strain CC9605) [Complete proteome] [HAMAP]

Taxonomic identifier

110662 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	249 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Bifunctional enzyme that catalyzes the enolization of 2,3-diketo-5-methylthiopentyl-1-phosphate (DK-MTP-1-P) into the intermediate 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate (HK-MTPenyl-1-P), which is then dephosphorylated to form the acireductone 1,2-dihydroxy-3-keto-5-methylthiopentene (DHK-MTPene) By similarity.
Catalytic activity	5-(methylthio)-2,3-dioxopentyl phosphate + H2O = 1,2-dihydroxy-5-(methylthio)pent-1-en-3-one + phosphate. HAMAP MF_01681
Cofactor	Binds 1 magnesium ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 3/6. HAMAP MF_01681 Amino-acid biosynthesis; L-methionine biosynthesis via salvage pathway; L-methionine from S-methyl-5-thio-alpha-D-ribose 1-phosphate: step 4/6.
Subunit structure	Monomer By similarity.
Sequence similarities	Belongs to the HAD-like hydrolase superfamily. MasA/mtnC family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Methionine biosynthesis
Ligand	Magnesium Metal-binding
Molecular function	Hydrolase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	methionine biosynthetic process from S-adenosylmethionine Inferred from electronic annotation. Source: HAMAP methionine salvage Inferred from electronic annotation. Source: InterPro
Molecular function	2,3-diketo-5-methylthiopentyl-1-phosphate enolase activity Inferred from electronic annotation. Source: HAMAP 2-hydroxy-3-keto-5-methylthiopentenyl-1-phosphate phosphatase activity Inferred from electronic annotation. Source: HAMAP acireductone synthase activity Inferred from electronic annotation. Source: EC magnesium ion binding Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 249	249	Enolase-phosphatase E1 HAMAP MF_01681		PRO_0000357421

Sequences

	Sequence		Length	Mass (Da)
	Q3AMB9-1 [UniParc]. Last modified December 16, 2008. Version 2. Checksum: D18F7C56014342D7	FASTA	249	28,621
	10 20 30 40 50 60 MNQQHLLLDI EGTTCPVRFV SDTLFPFAKK ELSRYITQNW DKRPHSKSIR AAWKEWMDDQ 70 80 90 100 110 120 SAESMIIKQQ VTQCEIEEVE GLIQYLKHLI SIDRKSTALK DLQGKIWEYG YGNGELKSQL 130 140 150 160 170 180 FPETAVCLRQ WHEQGLTLSV YSSGSIQAQK LLYRHSLNGN LEDLFSHWFD THTGPKKSAE 190 200 210 220 230 240 SYTTIAKQLQ SSPNKIWFVS DNGDECNSAR LAGMHTLFSL REGNPDRDPR DHKVVHSLRE VSALLIAEQ

References

[1]

"Complete sequence of Synechococcus sp. CC9605." Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Martinez M., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000110 Genomic DNA. Translation: ABB34263.1. Different initiation.
RefSeq	YP_380818.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AMB9.
Genome annotation databases
GeneID	3736936.
GenomeReviews	Gene locus Syncc9605_0489 in contig CP000110_GR.
KEGG	syd:Syncc9605_0489.
NMPDR	fig|110662.3.peg.1654.
Organism-specific databases
CMR	Search...
Phylogenomic databases
HOGENOM	Q3AMB9.
OMA	TTDLNFI.
Enzyme and pathway databases
BioCyc	SSP1131:SYNCC9605_0489-MON.
Family and domain databases
HAMAP	MF_01681. [Tree]
InterPro	IPR005834. Dehalogen-like_hydro. IPR010041. Enolase_ppase. [Graphical view]
PANTHER	PTHR20371. Enolase_ppase. 1 hit.
Pfam	PF00702. Hydrolase. 1 hit. [Graphical view]
TIGRFAMs	TIGR01691. enolase-ppase. 1 hit.
ProtoNet	Search...

Entry information

Entry name

MTNC_SYNSC

Accession

Primary (citable) accession number: Q3AMB9

Entry history

Integrated into UniProtKB/Swiss-Prot:	December 16, 2008
Last sequence update:	December 16, 2008
Last modified:	November 3, 2009
This is version 34 of the entry and version 2 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)