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Protein

2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase

Gene

ispD

Organism
Synechococcus sp. (strain CC9605)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the formation of 4-diphosphocytidyl-2-C-methyl-D-erythritol from CTP and 2-C-methyl-D-erythritol 4-phosphate (MEP).UniRule annotation

Catalytic activityi

CTP + 2-C-methyl-D-erythritol 4-phosphate = diphosphate + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol.UniRule annotation

Pathwayi: isopentenyl diphosphate biosynthesis via DXP pathway

This protein is involved in step 2 of the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate.UniRule annotation
Proteins known to be involved in the 6 steps of the subpathway in this organism are:
  1. 1-deoxy-D-xylulose 5-phosphate reductoisomerase (dxr)
  2. 2-C-methyl-D-erythritol 4-phosphate cytidylyltransferase (ispD)
  3. 4-diphosphocytidyl-2-C-methyl-D-erythritol kinase (ispE)
  4. Multifunctional fusion protein (trmD)
  5. 4-hydroxy-3-methylbut-2-en-1-yl diphosphate synthase (ferredoxin) (ispG)
  6. 4-hydroxy-3-methylbut-2-enyl diphosphate reductase (ispH)
This subpathway is part of the pathway isopentenyl diphosphate biosynthesis via DXP pathway, which is itself part of Isoprenoid biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate, the pathway isopentenyl diphosphate biosynthesis via DXP pathway and in Isoprenoid biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei13Transition state stabilizerUniRule annotation1
Sitei20Transition state stabilizerUniRule annotation1
Sitei150Positions MEP for the nucleophilic attackUniRule annotation1
Sitei206Positions MEP for the nucleophilic attackUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionNucleotidyltransferase, Transferase
Biological processIsoprene biosynthesis

Enzyme and pathway databases

BioCyciSSP110662:G1G50-650-MONOMER
UniPathwayiUPA00056; UER00093

Names & Taxonomyi

Protein namesi
Recommended name:
2-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseUniRule annotation (EC:2.7.7.60UniRule annotation)
Alternative name(s):
4-diphosphocytidyl-2C-methyl-D-erythritol synthaseUniRule annotation
MEP cytidylyltransferaseUniRule annotation
Short name:
MCTUniRule annotation
Gene namesi
Name:ispDUniRule annotation
Ordered Locus Names:Syncc9605_0620
OrganismiSynechococcus sp. (strain CC9605)
Taxonomic identifieri110662 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002711 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002378281 – 2262-C-methyl-D-erythritol 4-phosphate cytidylyltransferaseAdd BLAST226

Interactioni

Protein-protein interaction databases

STRINGi110662.Syncc9605_0620

Structurei

3D structure databases

ProteinModelPortaliQ3ALY8
SMRiQ3ALY8
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the IspD/TarI cytidylyltransferase family. IspD subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CE5 Bacteria
COG1211 LUCA
HOGENOMiHOG000218563
KOiK00991
OrthoDBiPOG091H066R

Family and domain databases

CDDicd02516 CDP-ME_synthetase, 1 hit
Gene3Di3.90.550.10, 1 hit
HAMAPiMF_00108 IspD, 1 hit
InterProiView protein in InterPro
IPR001228 IspD
IPR034683 IspD/TarI
IPR018294 ISPD_synthase_CS
IPR029044 Nucleotide-diphossugar_trans
PfamiView protein in Pfam
PF01128 IspD, 1 hit
SUPFAMiSSF53448 SSF53448, 1 hit
TIGRFAMsiTIGR00453 ispD, 1 hit
PROSITEiView protein in PROSITE
PS01295 ISPD, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ALY8-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MHLLIAAAGS GRRMGADRNK LLLPLAGRPV IAWTIEAALR SERIEWIGIV
60 70 80 90 100
GQEVDREAIL AVLDEPSKPV HWIQGGSTRQ ESVLCGLAGL PEQARHVLIH
110 120 130 140 150
DGARCLAEPK LFDRCAAVVE DGTALIAATP VSDTIKRVGS DGVIRDTPDR
160 170 180 190 200
SELWAAQTPQ GFAVDQLRRG HAEAVAQGWT VTDDASLYER LGWPVQVLDA
210 220
GPANIKVTTP FDLTVAEAVL ALRSAG
Length:226
Mass (Da):24,200
Last modified:May 30, 2006 - v2
Checksum:iA5A4CF453B9B52D0
GO

Sequence cautioni

The sequence ABB34394 differs from that shown. Reason: Erroneous initiation.Curated

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000110 Genomic DNA Translation: ABB34394.1 Different initiation.
RefSeqiWP_041434422.1, NC_007516.1

Genome annotation databases

EnsemblBacteriaiABB34394; ABB34394; Syncc9605_0620
KEGGisyd:Syncc9605_0620

Similar proteinsi

Entry informationi

Entry nameiISPD_SYNSC
AccessioniPrimary (citable) accession number: Q3ALY8
Entry historyiIntegrated into UniProtKB/Swiss-Prot: May 30, 2006
Last sequence update: May 30, 2006
Last modified: April 25, 2018
This is version 74 of the entry and version 2 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health