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Protein

3-methyl-2-oxobutanoate hydroxymethyltransferase

Gene

panB

Organism
Synechococcus sp. (strain CC9605)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the reversible reaction in which hydroxymethyl group from 5,10-methylenetetrahydrofolate is transferred onto alpha-ketoisovalerate to form ketopantoate.UniRule annotation

Catalytic activityi

5,10-methylenetetrahydrofolate + 3-methyl-2-oxobutanoate + H2O = tetrahydrofolate + 2-dehydropantoate.UniRule annotation

Cofactori

Mg2+UniRule annotationNote: Binds 1 Mg2+ ion per subunit.UniRule annotation

Pathwayi: (R)-pantothenate biosynthesis

This protein is involved in step 1 of the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. 3-methyl-2-oxobutanoate hydroxymethyltransferase (panB)
  2. no protein annotated in this organism
This subpathway is part of the pathway (R)-pantothenate biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes (R)-pantoate from 3-methyl-2-oxobutanoate, the pathway (R)-pantothenate biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi42MagnesiumUniRule annotation1
Metal bindingi86MagnesiumUniRule annotation1
Binding sitei86Alpha-ketoisovalerateUniRule annotation1
Binding sitei116Alpha-ketoisovalerateUniRule annotation1
Metal bindingi118MagnesiumUniRule annotation1
Active sitei185Proton acceptorUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processPantothenate biosynthesis
LigandMagnesium, Metal-binding

Enzyme and pathway databases

BioCyciSSP110662:G1G50-885-MONOMER
UniPathwayiUPA00028; UER00003

Names & Taxonomyi

Protein namesi
Recommended name:
3-methyl-2-oxobutanoate hydroxymethyltransferaseUniRule annotation (EC:2.1.2.11UniRule annotation)
Alternative name(s):
Ketopantoate hydroxymethyltransferaseUniRule annotation
Short name:
KPHMTUniRule annotation
Gene namesi
Name:panBUniRule annotation
Ordered Locus Names:Syncc9605_0845
OrganismiSynechococcus sp. (strain CC9605)
Taxonomic identifieri110662 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002711 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00002973891 – 2713-methyl-2-oxobutanoate hydroxymethyltransferaseAdd BLAST271

Interactioni

Subunit structurei

Homodecamer; pentamer of dimers.UniRule annotation

Protein-protein interaction databases

STRINGi110662.Syncc9605_0845

Structurei

3D structure databases

ProteinModelPortaliQ3ALB9
SMRiQ3ALB9
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Region

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Regioni42 – 43Alpha-ketoisovalerate bindingUniRule annotation2

Sequence similaritiesi

Belongs to the PanB family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CCG Bacteria
COG0413 LUCA
HOGENOMiHOG000078427
KOiK00606
OMAiGHIGLMP
OrthoDBiPOG091H02K8

Family and domain databases

CDDicd06557 KPHMT-like, 1 hit
HAMAPiMF_00156 PanB, 1 hit
InterProiView protein in InterPro
IPR003700 Pantoate_hydroxy_MeTrfase
IPR015813 Pyrv/PenolPyrv_Kinase-like_dom
PANTHERiPTHR20881 PTHR20881, 1 hit
PfamiView protein in Pfam
PF02548 Pantoate_transf, 1 hit
PIRSFiPIRSF000388 Pantoate_hydroxy_MeTrfase, 1 hit
SUPFAMiSSF51621 SSF51621, 1 hit
TIGRFAMsiTIGR00222 panB, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ALB9-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MRPSDLTRFK QKGQPIAVLT AWDSLSAALA EAAGADVLLI GDSLAMVALG
60 70 80 90 100
HATTLPVSLD QMLHHTQAVA RGLTTMPADQ PLLVCDLPFL SYQCGEDRAV
110 120 130 140 150
AAAGRLLKES SAAAVKLEGA EPEVVAVIDR LVRMGIPVMG HLGLTPQAVH
160 170 180 190 200
RLGYRRQATD AISQERLLEQ ACTLEQKGCF SLVLEHVPAE LACRVQQALT
210 220 230 240 250
IPVIGIGAGD DCDGQVRVTA DLLGLTAKQP PFSPALVDGR RLFIDALKGW
260 270
VNQTRNHTPP TNGRITQADR T
Length:271
Mass (Da):28,899
Last modified:November 22, 2005 - v1
Checksum:i4D9CF92F41AA792C
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000110 Genomic DNA Translation: ABB34613.1
RefSeqiWP_011363838.1, NC_007516.1

Genome annotation databases

EnsemblBacteriaiABB34613; ABB34613; Syncc9605_0845
KEGGisyd:Syncc9605_0845

Similar proteinsi

Entry informationi

Entry nameiPANB_SYNSC
AccessioniPrimary (citable) accession number: Q3ALB9
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 24, 2007
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 79 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome
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Main funding by: National Institutes of Health