Q3AKD9 (ISPE_SYNSC) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
4-diphosphocytidyl-2-C-methyl-D-erythritol kinase
Short name=CMK
EC=2.7.1.148

Alternative name(s):
4-(cytidine-5'-diphospho)-2-C-methyl-D-erythritol kinase

Gene names

Name:	ispE
Ordered Locus Names:	Syncc9605_1188

Organism

Synechococcus sp. (strain CC9605) [Complete proteome] [HAMAP]

Taxonomic identifier

110662 [NCBI]

Taxonomic lineage

Bacteria › Cyanobacteria › Chroococcales › Synechococcus

Protein attributes

Sequence length	307 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Catalyzes the phosphorylation of the position 2 hydroxy group of 4-diphosphocytidyl-2C-methyl-D-erythritol By similarity. HAMAP MF_00061
Catalytic activity	ATP + 4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol = ADP + 2-phospho-4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol. HAMAP MF_00061
Pathway	Isoprenoid biosynthesis; isopentenyl diphosphate biosynthesis via DXP pathway; isopentenyl diphosphate from 1-deoxy-D-xylulose 5-phosphate: step 3/6. HAMAP MF_00061
Sequence similarities	Belongs to the GHMP kinase family. IspE subfamily.

Ontologies

Keywords
Biological process	Isoprene biosynthesis
Ligand	ATP-binding Nucleotide-binding
Molecular function	Kinase Transferase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	terpenoid biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase activity Inferred from electronic annotation. Source: EC ATP binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 307	307	4-diphosphocytidyl-2-C-methyl-D-erythritol kinase HAMAP MF_00061		PRO_0000235140
Regions
	Nucleotide binding	94 – 104	11	ATP Potential
Sites
	Active site	9	1	By similarity
	Active site	136	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AKD9 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 2FCFEF15FFB70C35	FASTA	307	32,695
	10 20 30 40 50 60 MITVSAPAKV NLHLEVLGLR SDGFHELAMV MQSIDLADRL SFQNTADAQL SLTCDDASLS 70 80 90 100 110 120 VGDDNLILRA AQLLRDRSGF SELGASIHLE KRIPIGAGLA GGSSDGAAAL VGLNALWGLG 130 140 150 160 170 180 HSTADLERMA AELGSDMPFC VAGGCQLCFG RGEQLEAVPP TPQPLAVLLV KDPTVSVSTP 190 200 210 220 230 240 WAYKRCRELK QSHYLADEAA FEQRRQALRS VDWLQPLRSD LPPPLRNDLQ DVVAPETAAV 250 260 270 280 290 300 RSALDLLDSV PQSLAVAMSG SGPSCFGLFS DLASCRHAQD QLATQLERAG LKAWSCALRS DGVRIEA

References

[1]

"Complete sequence of Synechococcus sp. CC9605." US DOE Joint Genome Institute Copeland A., Lucas S., Lapidus A., Barry K., Detter J.C., Glavina T., Hammon N., Israni S., Pitluck S., Schmutz J., Martinez M., Larimer F., Land M., Kyrpides N., Ivanova N., Richardson P. Submitted (JUL-2005) to the EMBL/GenBank/DDBJ databases Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: CC9605.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000110 Genomic DNA. Translation: ABB34943.1.
RefSeq	YP_381498.1. NC_007516.1.
3D structure databases
ProteinModelPortal	Q3AKD9.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AKD9.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3735877.
GenomeReviews	Gene locus Syncc9605_1188 in contig CP000110_GR.
KEGG	syd:Syncc9605_1188.
NMPDR	fig|110662.3.peg.157.
Organism-specific databases
CMR	Search...
Phylogenomic databases
eggNOG	COG1947.
HOGENOM	HBG734593.
OMA	QLCFGRG.
PhylomeDB	Q3AKD9.
ProtClustDB	PRK02534.
Enzyme and pathway databases
BioCyc	SSP1131:SYNCC9605_1188-MONOMER.
Family and domain databases
HAMAP	MF_00061. IspE. [Tree]
InterPro	IPR006204. GHMP_kinase. IPR013750. GHMP_kinase_C. IPR004424. IspE. IPR020568. Ribosomal_S5_D2-typ_fold. IPR014721. Ribosomal_S5_D2-typ_fold_subgr. [Graphical view]
Gene3D	G3DSA:3.30.230.10. Ribosomal_S5_D2-type_fold. 1 hit.
KO	K00919.
PANTHER	PTHR20861:SF2. IspE. 1 hit.
Pfam	PF08544. GHMP_kinases_C. 1 hit. PF00288. GHMP_kinases_N. 1 hit. [Graphical view]
PIRSF	PIRSF010376. IspE. 1 hit.
SUPFAM	SSF54211. Ribosomal_S5_D2-typ_fold. 1 hit.
TIGRFAMs	TIGR00154. IspE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

ISPE_SYNSC

Accession

Primary (citable) accession number: Q3AKD9

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 16, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 39 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program