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Protein

Biotin synthase

Gene

bioB

Organism
Synechococcus sp. (strain CC9605)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical-based mechanism.UniRule annotation

Catalytic activityi

Dethiobiotin + sulfur-(sulfur carrier) + 2 S-adenosyl-L-methionine + 2 reduced [2Fe-2S] ferredoxin = biotin + (sulfur carrier) + 2 L-methionine + 2 5'-deoxyadenosine + 2 oxidized [2Fe-2S] ferredoxin.UniRule annotation

Cofactori

Protein has several cofactor binding sites:
  • [4Fe-4S] clusterUniRule annotationNote: Binds 1 [4Fe-4S] cluster. The cluster is coordinated with 3 cysteines and an exchangeable S-adenosyl-L-methionine.UniRule annotation
  • [2Fe-2S] clusterUniRule annotationNote: Binds 1 [2Fe-2S] cluster. The cluster is coordinated with 3 cysteines and 1 arginine.UniRule annotation

Pathwayi: biotin biosynthesis

This protein is involved in step 2 of the subpathway that synthesizes biotin from 7,8-diaminononanoate.UniRule annotation
Proteins known to be involved in the 2 steps of the subpathway in this organism are:
  1. ATP-dependent dethiobiotin synthetase BioD (bioD)
  2. Biotin synthase (bioB)
This subpathway is part of the pathway biotin biosynthesis, which is itself part of Cofactor biosynthesis.
View all proteins of this organism that are known to be involved in the subpathway that synthesizes biotin from 7,8-diaminononanoate, the pathway biotin biosynthesis and in Cofactor biosynthesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi55Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi59Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi62Iron-sulfur 1 (4Fe-4S-S-AdoMet)UniRule annotation1
Metal bindingi100Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi132Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi192Iron-sulfur 2 (2Fe-2S)UniRule annotation1
Metal bindingi264Iron-sulfur 2 (2Fe-2S)UniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionTransferase
Biological processBiotin biosynthesis
Ligand2Fe-2S, 4Fe-4S, Iron, Iron-sulfur, Metal-binding, S-adenosyl-L-methionine

Enzyme and pathway databases

BioCyciSSP110662:G1G50-1692-MONOMER
UniPathwayiUPA00078; UER00162

Names & Taxonomyi

Protein namesi
Recommended name:
Biotin synthaseUniRule annotation (EC:2.8.1.6UniRule annotation)
Gene namesi
Name:bioBUniRule annotation
Ordered Locus Names:Syncc9605_1632
OrganismiSynechococcus sp. (strain CC9605)
Taxonomic identifieri110662 [NCBI]
Taxonomic lineageiBacteriaCyanobacteriaSynechococcalesSynechococcaceaeSynechococcus
Proteomesi
  • UP000002711 Componenti: Chromosome

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00003816821 – 331Biotin synthaseAdd BLAST331

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi110662.Syncc9605_1632

Structurei

3D structure databases

ProteinModelPortaliQ3AJ51
SMRiQ3AJ51
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Sequence similaritiesi

Belongs to the radical SAM superfamily. Biotin synthase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4107QSQ Bacteria
COG0502 LUCA
HOGENOMiHOG000239957
KOiK01012
OMAiADRFCMG
OrthoDBiPOG091H01DF

Family and domain databases

Gene3Di3.20.20.701 hit
HAMAPiMF_01694 BioB, 1 hit
InterProiView protein in InterPro
IPR013785 Aldolase_TIM
IPR010722 BATS_dom
IPR034416 BATS_domain_containing
IPR002684 Biotin_synth/BioAB
IPR024177 Biotin_synthase
IPR006638 Elp3/MiaB/NifB
IPR007197 rSAM
PANTHERiPTHR22976 PTHR22976, 1 hit
PfamiView protein in Pfam
PF06968 BATS, 1 hit
PF04055 Radical_SAM, 1 hit
PIRSFiPIRSF001619 Biotin_synth, 1 hit
SFLDiSFLDF00272 biotin_synthase, 1 hit
SFLDG01060 BATS_domain_containing, 1 hit
SFLDG01278 biotin_synthase_like, 1 hit
SFLDS00029 Radical_SAM, 1 hit
SMARTiView protein in SMART
SM00876 BATS, 1 hit
SM00729 Elp3, 1 hit
TIGRFAMsiTIGR00433 bioB, 1 hit

Sequencei

Sequence statusi: Complete.

Q3AJ51-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MTLSIRHDWT TEEIQTLLEL PLMELLWEAQ TVHRKANPGY RVQLASLLSV
60 70 80 90 100
KTGGCEEDCA YCSQSIHNSS DVTAFEAQMQ VEPVLQRARA AKEAGADRFC
110 120 130 140 150
MGWAWREIRD GAPFEAMLEM VRGVRGMGME ACVTAGMLTD QQAERLAEAG
160 170 180 190 200
LTAYNHNLDT SPEHYDRIIS TRTYQERLET LERVRKAGVT LCCGGIIGMG
210 220 230 240 250
ETLRDRASML QVLASMNPHP ESVPVNGLVA VEGTPLEDQA PFEPLELVRM
260 270 280 290 300
VATARILMPH ARVRLSAGRE SMSREAQILC LQAGADSIFY GDVLLTTGNP
310 320 330
DVEADRQLLS DAGVTANWQE ETAAPVNCSP R
Length:331
Mass (Da):36,462
Last modified:November 22, 2005 - v1
Checksum:iAB438A67024C1D5D
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000110 Genomic DNA Translation: ABB35381.1
RefSeqiWP_011364592.1, NC_007516.1

Genome annotation databases

EnsemblBacteriaiABB35381; ABB35381; Syncc9605_1632
KEGGisyd:Syncc9605_1632

Similar proteinsi

Entry informationi

Entry nameiBIOB_SYNSC
AccessioniPrimary (citable) accession number: Q3AJ51
Entry historyiIntegrated into UniProtKB/Swiss-Prot: July 28, 2009
Last sequence update: November 22, 2005
Last modified: April 25, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome