Skip Header

You are using a version of browser that may not display all the features of this website. Please consider upgrading your browser.
Protein

K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.UniRule annotation1 Publication

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Sitei180Important for ion transportBy similarity1
Binding sitei188SubstrateBy similarity1
Metal bindingi191Magnesium 1By similarity1
Metal bindingi195Magnesium 1By similarity1
Metal bindingi218Magnesium 2By similarity1
Metal bindingi221Magnesium 2By similarity1
Sitei225Important for ion transportBy similarity1
Sitei232Important for ion transportBy similarity1
Metal bindingi430Magnesium 2By similarity1
Sitei460Important for potassium dependence1
Metal bindingi626Calcium or magnesiumBy similarity1
Metal bindingi652Calcium or magnesiumBy similarity1
Metal bindingi656Calcium or magnesiumBy similarity1
Binding sitei659SubstrateBy similarity1
Sitei660Important for ion transportBy similarity1
Sitei671Important for ion transportBy similarity1

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-stimulated pyrophosphate-energized sodium pump (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
Na(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CHY_0286
OrganismiCarboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
Proteomesi
  • UP000002706 Componenti: Chromosome

Subcellular locationi

  • Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Transmembranei6 – 26HelicalUniRule annotationAdd BLAST21
Transmembranei58 – 78HelicalUniRule annotationAdd BLAST21
Transmembranei89 – 109HelicalUniRule annotationAdd BLAST21
Transmembranei139 – 159HelicalUniRule annotationAdd BLAST21
Transmembranei165 – 185HelicalUniRule annotationAdd BLAST21
Transmembranei231 – 251HelicalUniRule annotationAdd BLAST21
Transmembranei264 – 284HelicalUniRule annotationAdd BLAST21
Transmembranei294 – 314HelicalUniRule annotationAdd BLAST21
Transmembranei327 – 347HelicalUniRule annotationAdd BLAST21
Transmembranei386 – 406HelicalUniRule annotationAdd BLAST21
Transmembranei408 – 428HelicalUniRule annotationAdd BLAST21
Transmembranei459 – 479HelicalUniRule annotationAdd BLAST21
Transmembranei509 – 529HelicalUniRule annotationAdd BLAST21
Transmembranei577 – 597HelicalUniRule annotationAdd BLAST21
Transmembranei599 – 619HelicalUniRule annotationAdd BLAST21
Transmembranei666 – 686HelicalUniRule annotationAdd BLAST21

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Mutagenesisi460A → K: Confers K(+) independence to both pyrophosphate hydrolysis and pyrophosphate-energized H(+) translocation. 1 Publication1
Mutagenesisi463A → T: Does not affect the K(+) dependence of H(+)-PPase. Decreased affinity for K(+). 1 Publication1

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_00004010661 – 686K(+)-stimulated pyrophosphate-energized sodium pumpAdd BLAST686

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0286.

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiMMAVGKA.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3AFC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENGMTLAYY GLGAGILAIL FALYLFSSVL KEDMGNEKMR EISQAIFEGA
60 70 80 90 100
MAYLNRQYKT LIPFALVVFV LLVVGFGYKE GDFGYGLKVG VSFLVGAIAS
110 120 130 140 150
ALAGYAGMTS TTKANARTTQ AARKSLNAAL NVAFRAGGVM GMSVAGLGLL
160 170 180 190 200
GVSALYIIFK DVHVIDSFAF GASAIAFFAR VGGGIYTKAA DVGADLVGKV
210 220 230 240 250
EAGIPEDDPR NPAVIADNVG DNVGDTAGMG ADLFESYGAT TMAAMLLGLT
260 270 280 290 300
FAKNHGFSEV LGATFPLLLG AAGIVAAIIS TFFVRTSEDG NPQMALNIGL
310 320 330 340 350
WSTNFITAIF TYIIAQYVFG SEWAPKIFIA VVSGLVVNVA IGSLTEYYTS
360 370 380 390 400
NLKPPAQKIA EASTTGPATN IISGIAVGMR STYLPIIVIV AAIMVGYWAA
410 420 430 440 450
GFYGIALAAM GMLATAAMVV AVDSFGPVAD NAGGIAEMAE LGPEIRNKTD
460 470 480 490 500
KLDAVGNTTA AVAKGFAIGS AALTALALFS AYTDLAKTNP NLQKYLVNGK
510 520 530 540 550
FDLNITDPWV LVGLFLGGTV AFLVAALTME SVGKAAFDMI EEVRRQFREI
560 570 580 590 600
PGLMEGKARP DYARCVSIST AAAIRQMIAP GLLAVGAPLA IGFILGFKAL
610 620 630 640 650
TGYLAGVTAT GVLLAIYMAN AGGAWDNAKK YIEAGNLGGK GSDTHKAAVV
660 670 680
GDTVGDPFKD TSGPAMNPLM KVAGTFALII VPLLLF
Length:686
Mass (Da):71,063
Last modified:November 22, 2005 - v1
Checksum:i39AAC4A0A6ECBD70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1.
RefSeqiWP_011343234.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
KEGGichy:CHY_0286.
PATRICi21273745. VBICarHyd26463_0272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1.
RefSeqiWP_011343234.1. NC_007503.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246194.CHY_0286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
KEGGichy:CHY_0286.
PATRICi21273745. VBICarHyd26463_0272.

Phylogenomic databases

eggNOGiENOG4105EAY. Bacteria.
COG3808. LUCA.
HOGENOMiHOG000007098.
KOiK15987.
OMAiMMAVGKA.
OrthoDBiPOG091H0DQP.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump. 1 hit.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Entry informationi

Entry nameiHPPA_CARHZ
AccessioniPrimary (citable) accession number: Q3AFC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 22, 2005
Last modified: November 2, 2016
This is version 55 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into one UniRef entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.