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Protein

K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Status
Reviewed-Annotation score: Annotation score: 4 out of 5-Experimental evidence at protein leveli

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.UniRule annotation1 Publication

Cofactori

Mg2+UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.UniRule annotation1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei180 – 1801Important for ion transportBy similarity
Binding sitei188 – 1881SubstrateBy similarity
Metal bindingi191 – 1911Magnesium 1By similarity
Metal bindingi195 – 1951Magnesium 1By similarity
Metal bindingi218 – 2181Magnesium 2By similarity
Metal bindingi221 – 2211Magnesium 2By similarity
Sitei225 – 2251Important for ion transportBy similarity
Sitei232 – 2321Important for ion transportBy similarity
Metal bindingi430 – 4301Magnesium 2By similarity
Sitei460 – 4601Important for potassium dependence
Metal bindingi626 – 6261Calcium or magnesiumBy similarity
Metal bindingi652 – 6521Calcium or magnesiumBy similarity
Metal bindingi656 – 6561Calcium or magnesiumBy similarity
Binding sitei659 – 6591SubstrateBy similarity
Sitei660 – 6601Important for ion transportBy similarity
Sitei671 – 6711Important for ion transportBy similarity

GO - Molecular functioni

  1. hydrogen-translocating pyrophosphatase activity Source: TIGR
  2. inorganic diphosphatase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. potassium ion binding Source: UniProtKB-HAMAP

GO - Biological processi

  1. proton transport Source: TIGR
  2. sodium ion transport Source: UniProtKB-HAMAP
  3. transmembrane transport Source: GOC
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-287-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-stimulated pyrophosphate-energized sodium pump (EC:3.6.1.1UniRule annotation)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
Short name:
Na(+)-PPaseUniRule annotation
Gene namesi
Name:hppAUniRule annotation
Ordered Locus Names:CHY_0286
OrganismiCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
ProteomesiUP000002706 Componenti: Chromosome

Subcellular locationi

  1. Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621HelicalUniRule annotationAdd
BLAST
Transmembranei58 – 7821HelicalUniRule annotationAdd
BLAST
Transmembranei89 – 10921HelicalUniRule annotationAdd
BLAST
Transmembranei139 – 15921HelicalUniRule annotationAdd
BLAST
Transmembranei165 – 18521HelicalUniRule annotationAdd
BLAST
Transmembranei231 – 25121HelicalUniRule annotationAdd
BLAST
Transmembranei264 – 28421HelicalUniRule annotationAdd
BLAST
Transmembranei294 – 31421HelicalUniRule annotationAdd
BLAST
Transmembranei327 – 34721HelicalUniRule annotationAdd
BLAST
Transmembranei386 – 40621HelicalUniRule annotationAdd
BLAST
Transmembranei408 – 42821HelicalUniRule annotationAdd
BLAST
Transmembranei459 – 47921HelicalUniRule annotationAdd
BLAST
Transmembranei509 – 52921HelicalUniRule annotationAdd
BLAST
Transmembranei577 – 59721HelicalUniRule annotationAdd
BLAST
Transmembranei599 – 61921HelicalUniRule annotationAdd
BLAST
Transmembranei666 – 68621HelicalUniRule annotationAdd
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi460 – 4601A → K: Confers K(+) independence to both pyrophosphate hydrolysis and pyrophosphate-energized H(+) translocation. 1 Publication
Mutagenesisi463 – 4631A → T: Does not affect the K(+) dependence of H(+)-PPase. Decreased affinity for K(+). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686K(+)-stimulated pyrophosphate-energized sodium pumpPRO_0000401066Add
BLAST

Interactioni

Subunit structurei

Homodimer.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0286.

Family & Domainsi

Sequence similaritiesi

Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3808.
HOGENOMiHOG000007098.
KOiK15987.
OMAiWFIVSKV.
OrthoDBiEOG6TXQSF.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3AFC6-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MENGMTLAYY GLGAGILAIL FALYLFSSVL KEDMGNEKMR EISQAIFEGA
60 70 80 90 100
MAYLNRQYKT LIPFALVVFV LLVVGFGYKE GDFGYGLKVG VSFLVGAIAS
110 120 130 140 150
ALAGYAGMTS TTKANARTTQ AARKSLNAAL NVAFRAGGVM GMSVAGLGLL
160 170 180 190 200
GVSALYIIFK DVHVIDSFAF GASAIAFFAR VGGGIYTKAA DVGADLVGKV
210 220 230 240 250
EAGIPEDDPR NPAVIADNVG DNVGDTAGMG ADLFESYGAT TMAAMLLGLT
260 270 280 290 300
FAKNHGFSEV LGATFPLLLG AAGIVAAIIS TFFVRTSEDG NPQMALNIGL
310 320 330 340 350
WSTNFITAIF TYIIAQYVFG SEWAPKIFIA VVSGLVVNVA IGSLTEYYTS
360 370 380 390 400
NLKPPAQKIA EASTTGPATN IISGIAVGMR STYLPIIVIV AAIMVGYWAA
410 420 430 440 450
GFYGIALAAM GMLATAAMVV AVDSFGPVAD NAGGIAEMAE LGPEIRNKTD
460 470 480 490 500
KLDAVGNTTA AVAKGFAIGS AALTALALFS AYTDLAKTNP NLQKYLVNGK
510 520 530 540 550
FDLNITDPWV LVGLFLGGTV AFLVAALTME SVGKAAFDMI EEVRRQFREI
560 570 580 590 600
PGLMEGKARP DYARCVSIST AAAIRQMIAP GLLAVGAPLA IGFILGFKAL
610 620 630 640 650
TGYLAGVTAT GVLLAIYMAN AGGAWDNAKK YIEAGNLGGK GSDTHKAAVV
660 670 680
GDTVGDPFKD TSGPAMNPLM KVAGTFALII VPLLLF
Length:686
Mass (Da):71,063
Last modified:November 22, 2005 - v1
Checksum:i39AAC4A0A6ECBD70
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1.
RefSeqiYP_359158.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
KEGGichy:CHY_0286.
PATRICi21273745. VBICarHyd26463_0272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1.
RefSeqiYP_359158.1. NC_007503.1.

3D structure databases

ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246194.CHY_0286.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
KEGGichy:CHY_0286.
PATRICi21273745. VBICarHyd26463_0272.

Phylogenomic databases

eggNOGiCOG3808.
HOGENOMiHOG000007098.
KOiK15987.
OMAiWFIVSKV.
OrthoDBiEOG6TXQSF.

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-287-MONOMER.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.
ProtoNetiSearch...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z-2901 / DSM 6008.
  2. "A lysine substitute for K+. A460K mutation eliminates K+ dependence in H+-pyrophosphatase of Carboxydothermus hydrogenoformans."
    Belogurov G.A., Lahti R.
    J. Biol. Chem. 277:49651-49654(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF ALA-460 AND ALA-463, IDENTIFICATION OF SITE IMPORTANT FOR POTASSIUM DEPENDENCE.

Entry informationi

Entry nameiHPPA_CARHZ
AccessioniPrimary (citable) accession number: Q3AFC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 22, 2005
Last modified: April 1, 2015
This is version 48 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.