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Q3AFC6

- HPPA_CARHZ

UniProt

Q3AFC6 - HPPA_CARHZ

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Protein
K(+)-stimulated pyrophosphate-energized sodium pump
Gene
hppA, CHY_0286
Organism
Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Status
Reviewed - Annotation score: 4 out of 5 - Experimental evidence at protein leveli

Functioni

Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane By similarity.UniRule annotation

Catalytic activityi

Diphosphate + H2O = 2 phosphate.1 Publication

Cofactori

Magnesium By similarity.UniRule annotation

Enzyme regulationi

Requires K+ for maximal activity.1 Publication

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Sitei180 – 1801Important for ion transport By similarity
Binding sitei188 – 1881Substrate By similarity
Metal bindingi191 – 1911Magnesium 1 By similarity
Metal bindingi195 – 1951Magnesium 1 By similarity
Metal bindingi218 – 2181Magnesium 2 By similarity
Metal bindingi221 – 2211Magnesium 2 By similarity
Sitei225 – 2251Important for ion transport By similarity
Sitei232 – 2321Important for ion transport By similarity
Metal bindingi430 – 4301Magnesium 2 By similarity
Sitei460 – 4601Important for potassium dependence
Metal bindingi626 – 6261Calcium or magnesium By similarity
Metal bindingi652 – 6521Calcium or magnesium By similarity
Metal bindingi656 – 6561Calcium or magnesium By similarity
Binding sitei659 – 6591Substrate By similarity
Sitei660 – 6601Important for ion transport By similarity
Sitei671 – 6711Important for ion transport By similarity

GO - Molecular functioni

  1. hydrogen-translocating pyrophosphatase activity Source: UniProtKB-HAMAP
  2. inorganic diphosphatase activity Source: UniProtKB-HAMAP
  3. magnesium ion binding Source: UniProtKB-HAMAP
  4. potassium ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. proton transport Source: InterPro
  2. sodium ion transport Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Hydrolase

Keywords - Biological processi

Ion transport, Sodium transport, Transport

Keywords - Ligandi

Calcium, Magnesium, Metal-binding, Potassium, Sodium

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-287-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
K(+)-stimulated pyrophosphate-energized sodium pump (EC:3.6.1.1)
Alternative name(s):
Membrane-bound sodium-translocating pyrophosphatase
Pyrophosphate-energized inorganic pyrophosphatase
Short name:
Na(+)-PPase
Gene namesi
Name:hppA
Ordered Locus Names:CHY_0286
OrganismiCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
ProteomesiUP000002706: Chromosome

Subcellular locationi

Topology

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Transmembranei6 – 2621Helical; Reviewed prediction
Add
BLAST
Transmembranei58 – 7821Helical; Reviewed prediction
Add
BLAST
Transmembranei89 – 10921Helical; Reviewed prediction
Add
BLAST
Transmembranei139 – 15921Helical; Reviewed prediction
Add
BLAST
Transmembranei165 – 18521Helical; Reviewed prediction
Add
BLAST
Transmembranei231 – 25121Helical; Reviewed prediction
Add
BLAST
Transmembranei264 – 28421Helical; Reviewed prediction
Add
BLAST
Transmembranei294 – 31421Helical; Reviewed prediction
Add
BLAST
Transmembranei327 – 34721Helical; Reviewed prediction
Add
BLAST
Transmembranei386 – 40621Helical; Reviewed prediction
Add
BLAST
Transmembranei408 – 42821Helical; Reviewed prediction
Add
BLAST
Transmembranei459 – 47921Helical; Reviewed prediction
Add
BLAST
Transmembranei509 – 52921Helical; Reviewed prediction
Add
BLAST
Transmembranei577 – 59721Helical; Reviewed prediction
Add
BLAST
Transmembranei599 – 61921Helical; Reviewed prediction
Add
BLAST
Transmembranei666 – 68621Helical; Reviewed prediction
Add
BLAST

GO - Cellular componenti

  1. integral component of plasma membrane Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Cellular componenti

Cell membrane, Membrane

Pathology & Biotechi

Mutagenesis

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Mutagenesisi460 – 4601A → K: Confers K(+) independence to both pyrophosphate hydrolysis and pyrophosphate-energized H(+) translocation. 1 Publication
Mutagenesisi463 – 4631A → T: Does not affect the K(+) dependence of H(+)-PPase. Decreased affinity for K(+). 1 Publication

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 686686K(+)-stimulated pyrophosphate-energized sodium pumpUniRule annotation
PRO_0000401066Add
BLAST

Interactioni

Subunit structurei

Homodimer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0286.

Family & Domainsi

Sequence similaritiesi

Keywords - Domaini

Transmembrane, Transmembrane helix

Phylogenomic databases

eggNOGiCOG3808.
HOGENOMiHOG000007098.
KOiK15987.
OMAiQYKYLAA.
OrthoDBiEOG6TXQSF.

Family and domain databases

HAMAPiMF_01129. PPase_energized_pump.
InterProiIPR004131. PPase-energised_H-pump.
[Graphical view]
PfamiPF03030. H_PPase. 1 hit.
[Graphical view]
PIRSFiPIRSF001265. H+-PPase. 1 hit.
TIGRFAMsiTIGR01104. V_PPase. 1 hit.

Sequencei

Sequence statusi: Complete.

Q3AFC6-1 [UniParc]FASTAAdd to Basket

« Hide

MENGMTLAYY GLGAGILAIL FALYLFSSVL KEDMGNEKMR EISQAIFEGA    50
MAYLNRQYKT LIPFALVVFV LLVVGFGYKE GDFGYGLKVG VSFLVGAIAS 100
ALAGYAGMTS TTKANARTTQ AARKSLNAAL NVAFRAGGVM GMSVAGLGLL 150
GVSALYIIFK DVHVIDSFAF GASAIAFFAR VGGGIYTKAA DVGADLVGKV 200
EAGIPEDDPR NPAVIADNVG DNVGDTAGMG ADLFESYGAT TMAAMLLGLT 250
FAKNHGFSEV LGATFPLLLG AAGIVAAIIS TFFVRTSEDG NPQMALNIGL 300
WSTNFITAIF TYIIAQYVFG SEWAPKIFIA VVSGLVVNVA IGSLTEYYTS 350
NLKPPAQKIA EASTTGPATN IISGIAVGMR STYLPIIVIV AAIMVGYWAA 400
GFYGIALAAM GMLATAAMVV AVDSFGPVAD NAGGIAEMAE LGPEIRNKTD 450
KLDAVGNTTA AVAKGFAIGS AALTALALFS AYTDLAKTNP NLQKYLVNGK 500
FDLNITDPWV LVGLFLGGTV AFLVAALTME SVGKAAFDMI EEVRRQFREI 550
PGLMEGKARP DYARCVSIST AAAIRQMIAP GLLAVGAPLA IGFILGFKAL 600
TGYLAGVTAT GVLLAIYMAN AGGAWDNAKK YIEAGNLGGK GSDTHKAAVV 650
GDTVGDPFKD TSGPAMNPLM KVAGTFALII VPLLLF 686
Length:686
Mass (Da):71,063
Last modified:November 22, 2005 - v1
Checksum:i39AAC4A0A6ECBD70
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1.
RefSeqiYP_359158.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
GeneIDi3726517.
KEGGichy:CHY_0286.
PATRICi21273745. VBICarHyd26463_0272.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000141 Genomic DNA. Translation: ABB14908.1 .
RefSeqi YP_359158.1. NC_007503.1.

3D structure databases

ModBasei Search...

Protein-protein interaction databases

STRINGi 246194.CHY_0286.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB14908 ; ABB14908 ; CHY_0286 .
GeneIDi 3726517.
KEGGi chy:CHY_0286.
PATRICi 21273745. VBICarHyd26463_0272.

Phylogenomic databases

eggNOGi COG3808.
HOGENOMi HOG000007098.
KOi K15987.
OMAi QYKYLAA.
OrthoDBi EOG6TXQSF.

Enzyme and pathway databases

BioCyci CHYD246194:GJCN-287-MONOMER.

Family and domain databases

HAMAPi MF_01129. PPase_energized_pump.
InterProi IPR004131. PPase-energised_H-pump.
[Graphical view ]
Pfami PF03030. H_PPase. 1 hit.
[Graphical view ]
PIRSFi PIRSF001265. H+-PPase. 1 hit.
TIGRFAMsi TIGR01104. V_PPase. 1 hit.
ProtoNeti Search...

Publicationsi

« Hide 'large scale' publications
  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z-2901 / DSM 6008.
  2. "A lysine substitute for K+. A460K mutation eliminates K+ dependence in H+-pyrophosphatase of Carboxydothermus hydrogenoformans."
    Belogurov G.A., Lahti R.
    J. Biol. Chem. 277:49651-49654(2002) [PubMed] [Europe PMC] [Abstract]
    Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF ALA-460 AND ALA-463, IDENTIFICATION OF SITE IMPORTANT FOR POTASSIUM DEPENDENCE.

Entry informationi

Entry nameiHPPA_CARHZ
AccessioniPrimary (citable) accession number: Q3AFC6
Entry historyi
Integrated into UniProtKB/Swiss-Prot: November 2, 2010
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 44 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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