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Q3AFC6

- HPPA_CARHZ

UniProt

Q3AFC6 - HPPA_CARHZ

Protein

K(+)-stimulated pyrophosphate-energized sodium pump

Gene

hppA

Organism
Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Status
Reviewed - Annotation score: 4 out of 5- Experimental evidence at protein leveli
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    • History
      Entry version 45 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Sodium pump that utilizes the energy of pyrophosphate hydrolysis as the driving force for Na+ movement across the membrane.UniRule annotation

    Catalytic activityi

    Diphosphate + H2O = 2 phosphate.1 PublicationUniRule annotation

    Cofactori

    Magnesium.UniRule annotation

    Enzyme regulationi

    Requires K+ for maximal activity.1 PublicationUniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Sitei180 – 1801Important for ion transportBy similarity
    Binding sitei188 – 1881SubstrateBy similarity
    Metal bindingi191 – 1911Magnesium 1By similarity
    Metal bindingi195 – 1951Magnesium 1By similarity
    Metal bindingi218 – 2181Magnesium 2By similarity
    Metal bindingi221 – 2211Magnesium 2By similarity
    Sitei225 – 2251Important for ion transportBy similarity
    Sitei232 – 2321Important for ion transportBy similarity
    Metal bindingi430 – 4301Magnesium 2By similarity
    Sitei460 – 4601Important for potassium dependence
    Metal bindingi626 – 6261Calcium or magnesiumBy similarity
    Metal bindingi652 – 6521Calcium or magnesiumBy similarity
    Metal bindingi656 – 6561Calcium or magnesiumBy similarity
    Binding sitei659 – 6591SubstrateBy similarity
    Sitei660 – 6601Important for ion transportBy similarity
    Sitei671 – 6711Important for ion transportBy similarity

    GO - Molecular functioni

    1. hydrogen-translocating pyrophosphatase activity Source: UniProtKB-HAMAP
    2. inorganic diphosphatase activity Source: UniProtKB-HAMAP
    3. magnesium ion binding Source: UniProtKB-HAMAP
    4. potassium ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. proton transport Source: InterPro
    2. sodium ion transport Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Hydrolase

    Keywords - Biological processi

    Ion transport, Sodium transport, Transport

    Keywords - Ligandi

    Calcium, Magnesium, Metal-binding, Potassium, Sodium

    Enzyme and pathway databases

    BioCyciCHYD246194:GJCN-287-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    K(+)-stimulated pyrophosphate-energized sodium pump (EC:3.6.1.1UniRule annotation)
    Alternative name(s):
    Membrane-bound sodium-translocating pyrophosphataseUniRule annotation
    Pyrophosphate-energized inorganic pyrophosphataseUniRule annotation
    Short name:
    Na(+)-PPaseUniRule annotation
    Gene namesi
    Name:hppAUniRule annotation
    Ordered Locus Names:CHY_0286
    OrganismiCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
    Taxonomic identifieri246194 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
    ProteomesiUP000002706: Chromosome

    Subcellular locationi

    Cell membrane UniRule annotation; Multi-pass membrane protein UniRule annotation

    GO - Cellular componenti

    1. integral component of plasma membrane Source: UniProtKB-HAMAP

    Keywords - Cellular componenti

    Cell membrane, Membrane

    Pathology & Biotechi

    Mutagenesis

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Mutagenesisi460 – 4601A → K: Confers K(+) independence to both pyrophosphate hydrolysis and pyrophosphate-energized H(+) translocation. 1 Publication
    Mutagenesisi463 – 4631A → T: Does not affect the K(+) dependence of H(+)-PPase. Decreased affinity for K(+). 1 Publication

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 686686K(+)-stimulated pyrophosphate-energized sodium pumpPRO_0000401066Add
    BLAST

    Interactioni

    Subunit structurei

    Homodimer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246194.CHY_0286.

    Structurei

    Transmembrane

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Transmembranei6 – 2621HelicalUniRule annotationAdd
    BLAST
    Transmembranei58 – 7821HelicalUniRule annotationAdd
    BLAST
    Transmembranei89 – 10921HelicalUniRule annotationAdd
    BLAST
    Transmembranei139 – 15921HelicalUniRule annotationAdd
    BLAST
    Transmembranei165 – 18521HelicalUniRule annotationAdd
    BLAST
    Transmembranei231 – 25121HelicalUniRule annotationAdd
    BLAST
    Transmembranei264 – 28421HelicalUniRule annotationAdd
    BLAST
    Transmembranei294 – 31421HelicalUniRule annotationAdd
    BLAST
    Transmembranei327 – 34721HelicalUniRule annotationAdd
    BLAST
    Transmembranei386 – 40621HelicalUniRule annotationAdd
    BLAST
    Transmembranei408 – 42821HelicalUniRule annotationAdd
    BLAST
    Transmembranei459 – 47921HelicalUniRule annotationAdd
    BLAST
    Transmembranei509 – 52921HelicalUniRule annotationAdd
    BLAST
    Transmembranei577 – 59721HelicalUniRule annotationAdd
    BLAST
    Transmembranei599 – 61921HelicalUniRule annotationAdd
    BLAST
    Transmembranei666 – 68621HelicalUniRule annotationAdd
    BLAST

    Family & Domainsi

    Sequence similaritiesi

    Belongs to the H(+)-translocating pyrophosphatase (TC 3.A.10) family. K(+)-stimulated subfamily. [View classification]UniRule annotation

    Keywords - Domaini

    Transmembrane, Transmembrane helix

    Phylogenomic databases

    eggNOGiCOG3808.
    HOGENOMiHOG000007098.
    KOiK15987.
    OMAiQYKYLAA.
    OrthoDBiEOG6TXQSF.

    Family and domain databases

    HAMAPiMF_01129. PPase_energized_pump.
    InterProiIPR004131. PPase-energised_H-pump.
    [Graphical view]
    PfamiPF03030. H_PPase. 1 hit.
    [Graphical view]
    PIRSFiPIRSF001265. H+-PPase. 1 hit.
    TIGRFAMsiTIGR01104. V_PPase. 1 hit.

    Sequencei

    Sequence statusi: Complete.

    Q3AFC6-1 [UniParc]FASTAAdd to Basket

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    MENGMTLAYY GLGAGILAIL FALYLFSSVL KEDMGNEKMR EISQAIFEGA    50
    MAYLNRQYKT LIPFALVVFV LLVVGFGYKE GDFGYGLKVG VSFLVGAIAS 100
    ALAGYAGMTS TTKANARTTQ AARKSLNAAL NVAFRAGGVM GMSVAGLGLL 150
    GVSALYIIFK DVHVIDSFAF GASAIAFFAR VGGGIYTKAA DVGADLVGKV 200
    EAGIPEDDPR NPAVIADNVG DNVGDTAGMG ADLFESYGAT TMAAMLLGLT 250
    FAKNHGFSEV LGATFPLLLG AAGIVAAIIS TFFVRTSEDG NPQMALNIGL 300
    WSTNFITAIF TYIIAQYVFG SEWAPKIFIA VVSGLVVNVA IGSLTEYYTS 350
    NLKPPAQKIA EASTTGPATN IISGIAVGMR STYLPIIVIV AAIMVGYWAA 400
    GFYGIALAAM GMLATAAMVV AVDSFGPVAD NAGGIAEMAE LGPEIRNKTD 450
    KLDAVGNTTA AVAKGFAIGS AALTALALFS AYTDLAKTNP NLQKYLVNGK 500
    FDLNITDPWV LVGLFLGGTV AFLVAALTME SVGKAAFDMI EEVRRQFREI 550
    PGLMEGKARP DYARCVSIST AAAIRQMIAP GLLAVGAPLA IGFILGFKAL 600
    TGYLAGVTAT GVLLAIYMAN AGGAWDNAKK YIEAGNLGGK GSDTHKAAVV 650
    GDTVGDPFKD TSGPAMNPLM KVAGTFALII VPLLLF 686
    Length:686
    Mass (Da):71,063
    Last modified:November 22, 2005 - v1
    Checksum:i39AAC4A0A6ECBD70
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000141 Genomic DNA. Translation: ABB14908.1.
    RefSeqiYP_359158.1. NC_007503.1.

    Genome annotation databases

    EnsemblBacteriaiABB14908; ABB14908; CHY_0286.
    GeneIDi3726517.
    KEGGichy:CHY_0286.
    PATRICi21273745. VBICarHyd26463_0272.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000141 Genomic DNA. Translation: ABB14908.1 .
    RefSeqi YP_359158.1. NC_007503.1.

    3D structure databases

    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246194.CHY_0286.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB14908 ; ABB14908 ; CHY_0286 .
    GeneIDi 3726517.
    KEGGi chy:CHY_0286.
    PATRICi 21273745. VBICarHyd26463_0272.

    Phylogenomic databases

    eggNOGi COG3808.
    HOGENOMi HOG000007098.
    KOi K15987.
    OMAi QYKYLAA.
    OrthoDBi EOG6TXQSF.

    Enzyme and pathway databases

    BioCyci CHYD246194:GJCN-287-MONOMER.

    Family and domain databases

    HAMAPi MF_01129. PPase_energized_pump.
    InterProi IPR004131. PPase-energised_H-pump.
    [Graphical view ]
    Pfami PF03030. H_PPase. 1 hit.
    [Graphical view ]
    PIRSFi PIRSF001265. H+-PPase. 1 hit.
    TIGRFAMsi TIGR01104. V_PPase. 1 hit.
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Z-2901 / DSM 6008.
    2. "A lysine substitute for K+. A460K mutation eliminates K+ dependence in H+-pyrophosphatase of Carboxydothermus hydrogenoformans."
      Belogurov G.A., Lahti R.
      J. Biol. Chem. 277:49651-49654(2002) [PubMed] [Europe PMC] [Abstract]
      Cited for: CATALYTIC ACTIVITY, ENZYME REGULATION, MUTAGENESIS OF ALA-460 AND ALA-463, IDENTIFICATION OF SITE IMPORTANT FOR POTASSIUM DEPENDENCE.

    Entry informationi

    Entry nameiHPPA_CARHZ
    AccessioniPrimary (citable) accession number: Q3AFC6
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: November 2, 2010
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 45 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3