Reviewed, UniProtKB/Swiss-Prot Q3AF39 (PROA_CARHZ)

Names and origin

Protein names

Recommended name:
    Gamma-glutamyl phosphate reductase
      Short name=GPR
    EC=1.2.1.41
Alternative name(s):
    Glutamate-5-semialdehyde dehydrogenase
    Glutamyl-gamma-semialdehyde dehydrogenase
      Short name=GSA dehydrogenase

Gene names

Name:	proA
Ordered Locus Names:	CHY_0383

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	415 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the NADPH dependent reduction of L-gamma-glutamyl 5-phosphate into L-glutamate 5-semialdehyde and phosphate. The product spontaneously undergoes cyclization to form 1-pyrroline-5-carboxylate.
Catalytic activity	L-glutamate 5-semialdehyde + phosphate + NADP(+) = L-glutamyl 5-phosphate + NADPH.
Pathway	Amino-acid biosynthesis; L-proline biosynthesis; L-glutamate 5-semialdehyde from L-glutamate: step 2/2.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the gamma-glutamyl phosphate reductase family.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Proline biosynthesis
Cellular component	Cytoplasm
Ligand	NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW proline biosynthetic process Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	NADP binding Inferred from electronic annotation. Source: InterPro glutamate-5-semialdehyde dehydrogenase activity Inferred from electronic annotation. Source: HAMAP
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 415	415	Gamma-glutamyl phosphate reductase		PRO_0000229996

Sequences

	Sequence		Length	Mass (Da)
	Q3AF39-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 2F0BED5AA870B69D	FASTA	415	45,489
	10 20 30 40 50 60 MDEVLNLAKK AKEASKKLAQ LSTEQKNRAL LKIAQYLEEN MEKILTENQK DLAEAKKGGL 70 80 90 100 110 120 SPAFIERLTL NEKRILDMAE GVRQVAKLPD PVGEVLGMTR RPNGLVIGQV RVPLGVVGII 130 140 150 160 170 180 YESRPNVTVD AAALCLKAGN AVILRGGKEA FNSNLALVTL MEEALRSEEI PEGAVGMIKT 190 200 210 220 230 240 TSRDAANYLM RLNGYLDVLI PRGGAGLIKT VVENSTVPVI ETGVGNCHVY VEEDADLEMA 250 260 270 280 290 300 ERIIINAKCQ RPAVCNAMET LLVHEKIAPV FLPQIGKALK ENGVEIRGCE VTRRYIPDAL 310 320 330 340 350 360 PATEEDYYTE FLDLILAVRV VRDLDEAIAH ITKYGSGHSE AIVTRDYFKA RRFTEEVDAA 370 380 390 400 410 AVYVNASTRF TDGFEFGFGA EIGISTQKLH ARGPMGLKEL TTTKYVIYGT GQIRG

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000141 Genomic DNA. Translation: ABB13750.1.
RefSeq	YP_359245.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3726908.
GenomeReviews	Gene locus CHY_0383 in contig CP000141_GR.
KEGG	chy:CHY_0383.
NMPDR	fig|246194.3.peg.599.
TIGR	CHY_0383.
Phylogenomic databases
HOGENOM	Q3AF39.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_0383-MON.
Family and domain databases
HAMAP	MF_00412. [Tree]
InterPro	IPR016163. Ald_DHase_C. IPR016162. Ald_DHase_N. IPR015590. Aldehyde_DHase. IPR000965. Gglut_pp_reduct. IPR012134. Glu-5-SA_DHase. [Graphical view]
Gene3D	G3DSA:3.40.309.10. Aldehyde_dehydrogenase_C. 1 hit. G3DSA:3.40.605.10. Aldehyde_dehydrogenase_N. 1 hit.
PANTHER	PTHR11063:SF1. GSA_DH. 1 hit.
Pfam	PF00171. Aldedh. 1 hit. [Graphical view]
PIRSF	PIRSF000151. GPR. 1 hit.
TIGRFAMs	TIGR00407. proA. 1 hit.
PROSITE	PS01223. PROA. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

PROA_CARHZ

Accession

Primary (citable) accession number: Q3AF39

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 22, 2005
Last modified:	November 25, 2008
This is version 24 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)