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Protein

Leucine--tRNA ligase

Gene

leuS

Organism
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Status
Reviewed-Annotation score: Annotation score: 2 out of 5-Protein inferred from homologyi

Functioni

Catalytic activityi

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu).UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei586 – 5861ATPUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-394-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Leucine--tRNA ligaseUniRule annotation (EC:6.1.1.4UniRule annotation)
Alternative name(s):
Leucyl-tRNA synthetaseUniRule annotation
Short name:
LeuRSUniRule annotation
Gene namesi
Name:leuSUniRule annotation
Ordered Locus Names:CHY_0393
OrganismiCarboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
ProteomesiUP000002706 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 821821Leucine--tRNA ligasePRO_0000334741Add
BLAST

Interactioni

Protein-protein interaction databases

STRINGi246194.CHY_0393.

Structurei

3D structure databases

ProteinModelPortaliQ3AF30.
SMRiQ3AF30. Positions 3-755.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi42 – 5211"HIGH" regionAdd
BLAST
Motifi583 – 5875"KMSKS" region

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family.UniRule annotation

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3AF30-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MQERYDFKAI EAKWQKKWEE LKLYEVDDFS EKPKYYCLEM FPYPSGKLHM
60 70 80 90 100
GHVRNYSIGD VVARYKRMRG YAVLHPMGWD AFGLPAENAA IKHGNVHPAD
110 120 130 140 150
WTWDNIANMR RQLKELGISY DWRREIATCH PEYYRWTQWL FLQFYKKGLA
160 170 180 190 200
YKKKAPVNWC PGCQTVLANE QVIDGECERC HSRVEKKELE QWFFKITAYA
210 220 230 240 250
ERLLQDIKKL TGWPEKVKIM QENWIGRSEG AEITFKIKGH EETISVFTTR
260 270 280 290 300
PDTIFGVTYM VLAPEHPLVE KISRGTQYEK DVLEFKRKMM YLSEIERTAE
310 320 330 340 350
TAEKEGVFTG AYAINPFTGE EIPILLANYV LVEYGTGAVM GVPAHDQRDF
360 370 380 390 400
LFAKKYNLPI KVVITPPGQE LKAEELTEAY TGEGILVNSG EFTGLANKEA
410 420 430 440 450
IRIITQEAEK RGFGKYRVNY RLRDWLISRQ RYWGAPIPVL YCEKCGIVPV
460 470 480 490 500
PEDQLPVVLP YNVEFRPTGE SPLKYVPEFL NATCPECGGP ATRETDTMDT
510 520 530 540 550
FICSSWYYYR YTSPRDKQQP WSKEKVERWL PVDQYIGGVE HAILHLLYSR
560 570 580 590 600
FFTKVLYDLG LVHVDEPFTN LLTQGMVLKD GAKMSKSKGN VVSPEEIVEK
610 620 630 640 650
YGADTARLFI LFAAPPERDL EWSDQGVEGS FRFLNRVWRL IYQTKDRISD
660 670 680 690 700
ELREFSAKDK ELNRLLHATI KKVTEDIEER FNFNTAISAI MELVNGLYQY
710 720 730 740 750
KEGEINPGLL KEALNKLVIL LAPFAPHIAE ELWEALGNKQ SVHLEKWPEY
760 770 780 790 800
DEEALITEEV EIVIQVNGKV KDRVMVPRNA AEDELKEIAL NTPKIKELTA
810 820
DKKIIKVIIV PEKLINIVVA G
Length:821
Mass (Da):95,008
Last modified:November 22, 2005 - v1
Checksum:i52887B954D2593AB
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB15559.1.
RefSeqiWP_011343330.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB15559; ABB15559; CHY_0393.
KEGGichy:CHY_0393.
PATRICi21273949. VBICarHyd26463_0365.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB15559.1.
RefSeqiWP_011343330.1. NC_007503.1.

3D structure databases

ProteinModelPortaliQ3AF30.
SMRiQ3AF30. Positions 3-755.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246194.CHY_0393.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB15559; ABB15559; CHY_0393.
KEGGichy:CHY_0393.
PATRICi21273949. VBICarHyd26463_0365.

Phylogenomic databases

eggNOGiCOG0495.
HOGENOMiHOG000200747.
KOiK01869.
OMAiEGAHRFI.
OrthoDBiEOG63Z74X.

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-394-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_00049_B. Leu_tRNA_synth_B.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase.
IPR025709. Leu_tRNA-synth_edit.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERiPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSiPR00985. TRNASYNTHLEU.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00396. leuS_bact. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-161 / DSM 6008 / Z-2901.

Entry informationi

Entry nameiSYL_CARHZ
AccessioniPrimary (citable) accession number: Q3AF30
Entry historyi
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 22, 2005
Last modified: July 22, 2015
This is version 85 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.