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Q3AF30 (SYL_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Last modified April 16, 2014. Version 75. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
Leucine--tRNA ligase

EC=6.1.1.4
Alternative name(s):
Leucyl-tRNA synthetase
Short name=LeuRS
Gene names
Name:leuS
Ordered Locus Names:CHY_0393
OrganismCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]
Taxonomic identifier246194 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus

Protein attributes

Sequence length821 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Catalytic activity

ATP + L-leucine + tRNA(Leu) = AMP + diphosphate + L-leucyl-tRNA(Leu). HAMAP-Rule MF_00049

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00049.

Sequence similarities

Belongs to the class-I aminoacyl-tRNA synthetase family.

Ontologies

Keywords
   Biological processProtein biosynthesis
   Cellular componentCytoplasm
   LigandATP-binding
Nucleotide-binding
   Molecular functionAminoacyl-tRNA synthetase
Ligase
   Technical termComplete proteome
Gene Ontology (GO)
   Biological_processleucyl-tRNA aminoacylation

Inferred from electronic annotation. Source: UniProtKB-HAMAP

   Cellular_componentcytoplasm

Inferred from electronic annotation. Source: UniProtKB-SubCell

   Molecular_functionATP binding

Inferred from electronic annotation. Source: UniProtKB-HAMAP

aminoacyl-tRNA editing activity

Inferred from electronic annotation. Source: InterPro

leucine-tRNA ligase activity

Inferred from electronic annotation. Source: UniProtKB-HAMAP

Complete GO annotation...

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 821821Leucine--tRNA ligase HAMAP-Rule MF_00049
PRO_0000334741

Regions

Motif42 – 5211"HIGH" region HAMAP-Rule MF_00049
Motif583 – 5875"KMSKS" region HAMAP-Rule MF_00049

Sites

Binding site5861ATP By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3AF30 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: 52887B954D2593AB

FASTA82195,008
        10         20         30         40         50         60 
MQERYDFKAI EAKWQKKWEE LKLYEVDDFS EKPKYYCLEM FPYPSGKLHM GHVRNYSIGD 

        70         80         90        100        110        120 
VVARYKRMRG YAVLHPMGWD AFGLPAENAA IKHGNVHPAD WTWDNIANMR RQLKELGISY 

       130        140        150        160        170        180 
DWRREIATCH PEYYRWTQWL FLQFYKKGLA YKKKAPVNWC PGCQTVLANE QVIDGECERC 

       190        200        210        220        230        240 
HSRVEKKELE QWFFKITAYA ERLLQDIKKL TGWPEKVKIM QENWIGRSEG AEITFKIKGH 

       250        260        270        280        290        300 
EETISVFTTR PDTIFGVTYM VLAPEHPLVE KISRGTQYEK DVLEFKRKMM YLSEIERTAE 

       310        320        330        340        350        360 
TAEKEGVFTG AYAINPFTGE EIPILLANYV LVEYGTGAVM GVPAHDQRDF LFAKKYNLPI 

       370        380        390        400        410        420 
KVVITPPGQE LKAEELTEAY TGEGILVNSG EFTGLANKEA IRIITQEAEK RGFGKYRVNY 

       430        440        450        460        470        480 
RLRDWLISRQ RYWGAPIPVL YCEKCGIVPV PEDQLPVVLP YNVEFRPTGE SPLKYVPEFL 

       490        500        510        520        530        540 
NATCPECGGP ATRETDTMDT FICSSWYYYR YTSPRDKQQP WSKEKVERWL PVDQYIGGVE 

       550        560        570        580        590        600 
HAILHLLYSR FFTKVLYDLG LVHVDEPFTN LLTQGMVLKD GAKMSKSKGN VVSPEEIVEK 

       610        620        630        640        650        660 
YGADTARLFI LFAAPPERDL EWSDQGVEGS FRFLNRVWRL IYQTKDRISD ELREFSAKDK 

       670        680        690        700        710        720 
ELNRLLHATI KKVTEDIEER FNFNTAISAI MELVNGLYQY KEGEINPGLL KEALNKLVIL 

       730        740        750        760        770        780 
LAPFAPHIAE ELWEALGNKQ SVHLEKWPEY DEEALITEEV EIVIQVNGKV KDRVMVPRNA 

       790        800        810        820 
AEDELKEIAL NTPKIKELTA DKKIIKVIIV PEKLINIVVA G 

« Hide

References

[1]"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901."
Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A.
PLoS Genet. 1:563-574(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000141 Genomic DNA. Translation: ABB15559.1.
RefSeqYP_359254.1. NC_007503.1.

3D structure databases

ProteinModelPortalQ3AF30.
SMRQ3AF30. Positions 3-755.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246194.CHY_0393.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB15559; ABB15559; CHY_0393.
GeneID3727664.
KEGGchy:CHY_0393.
PATRIC21273949. VBICarHyd26463_0365.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG0495.
HOGENOMHOG000200747.
KOK01869.
OMAGIEHACM.
OrthoDBEOG63Z74X.
ProtClustDBPRK00390.

Enzyme and pathway databases

BioCycCHYD246194:GJCN-394-MONOMER.

Family and domain databases

Gene3D1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPMF_00049_B. Leu_tRNA_synth_B.
InterProIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002302. Leu-tRNA-ligase_bac/mito.
IPR025709. Leu_tRNA-synth_edit.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
[Graphical view]
PANTHERPTHR11946:SF7. PTHR11946:SF7. 1 hit.
PfamPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 2 hits.
PF13603. tRNA-synt_1_2. 1 hit.
[Graphical view]
PRINTSPR00985. TRNASYNTHLEU.
SUPFAMSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsTIGR00396. leuS_bact. 1 hit.
PROSITEPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameSYL_CARHZ
AccessionPrimary (citable) accession number: Q3AF30
Entry history
Integrated into UniProtKB/Swiss-Prot: May 20, 2008
Last sequence update: November 22, 2005
Last modified: April 16, 2014
This is version 75 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

Aminoacyl-tRNA synthetases

List of aminoacyl-tRNA synthetase entries