Q3AF08 (DNAK_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Chaperone protein DnaK

Alternative name(s):
HSP70
Heat shock 70 kDa protein
Heat shock protein 70

Gene names

Name:	dnaK
Ordered Locus Names:	CHY_0415

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	604 AA.
Sequence status	Complete.
Protein existence	Evidence at transcript level

General annotation (Comments)

Function	Acts as a chaperone By similarity. HAMAP MF_00332
Induction	By stress conditions e.g. heat shock By similarity. HAMAP MF_00332
Sequence similarities	Belongs to the heat shock protein 70 family.

Ontologies

Keywords
Biological process	Stress response
Ligand	ATP-binding Nucleotide-binding
Molecular function	Chaperone
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	protein folding Inferred from electronic annotation. Source: InterPro response to stress Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW unfolded protein binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 604	604	Chaperone protein DnaK HAMAP MF_00332		PRO_0000225948
Amino acid modifications
	Modified residue	174	1	Phosphothreonine; by autocatalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AF08 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 529A280014507FFA	FASTA	604	66,112
	10 20 30 40 50 60 MGRIVGIDLG TTNSCIAVME GGEVIVIPNA EGGRTTPSVV AFGKNGERIV GQVAKRQAIT 70 80 90 100 110 120 NPERTVISIK RHMGTNYRVK IDDKEYTPQE ISAMILQKLK QDAEAYLGEK IEKAVITVPA 130 140 150 160 170 180 YFTDAQRQAT KDAGRIAGLE VLRIINEPTA AALAYGLDKE GEQTILVYDL GGGTFDVSIL 190 200 210 220 230 240 EIGDGVFEVK ATAGNNRLGG DDFDQRIIDW LVENFKKEHG IDLRNDKMAM QRLKEAAEKA 250 260 270 280 290 300 KIELSGVLET QINLPFIAAN QNGPLHIDVT LTRAKFNELT ADLVEATMGP TRQALADAGL 310 320 330 340 350 360 KPEDIDKILL VGGSTRIPAV QEAIRKFFNK EPHKGINPDE CVAIGAAIQA AVLAGEVKDV 370 380 390 400 410 420 VLLDVTPLSL GIETLGGVFT KLIERNTTIP TSKSQIFTTA ADNQTSVEIH VLQGERPMAA 430 440 450 460 470 480 DNVSLGRFTL TGIPPAPRGV PQIEVRFDID VNGIVHVSAK DLGTGREQSI TITNTSNLSE 490 500 510 520 530 540 AEIKRMVEEA ERYAEEDRKR KEEVETRNQA DSLIYQAEKT LKDFKDKANP DLVARVEKAI 550 560 570 580 590 600 AELREAMNSK DVQLMRAKME ELTKPLYELT SSIYQQSANQ NQTQTGTNTQ GNVYDADYKV NDDK

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB16061.1.
RefSeq	YP_359276.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3AF08.
SMR	Q3AF08. Positions 1-505.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AF08.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3728173.
GenomeReviews	Gene locus CHY_0415 in contig CP000141_GR.
KEGG	chy:CHY_0415.
NMPDR	fig|246194.3.peg.748.
PATRIC	21273991. VBICarHyd26463_0386.
TIGR	CHY_0415.
Phylogenomic databases
eggNOG	COG0443.
HOGENOM	HBG334976.
OMA	IDTAMAE.
ProtClustDB	PRK00290.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_0415-MONOMER.
Family and domain databases
HAMAP	MF_00332. DnaK. [Tree]
InterPro	IPR012725. Chaperone_DnaK. IPR018181. Heat_shock_70_CS. IPR001023. Hsp70. IPR013126. Hsp_70. [Graphical view]
KO	K04043.
PANTHER	PTHR19375. Hsp70. 1 hit.
Pfam	PF00012. HSP70. 2 hits. [Graphical view]
PRINTS	PR00301. HEATSHOCK70.
TIGRFAMs	TIGR02350. Prok_dnaK. 1 hit.
PROSITE	PS00297. HSP70_1. 1 hit. PS00329. HSP70_2. 1 hit. PS01036. HSP70_3. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

DNAK_CARHZ

Accession

Primary (citable) accession number: Q3AF08

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program