Reviewed, UniProtKB/Swiss-Prot Q3AET5 (GCSPA_CARHZ)

Names and origin

Protein names

Recommended name:
    Probable glycine dehydrogenase [decarboxylating] subunit 1
    EC=1.4.4.2
Alternative name(s):
    Glycine decarboxylase subunit 1
    Glycine cleavage system P-protein subunit 1

Gene names

Name:	gcvPA
Ordered Locus Names:	CHY_0491

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	444 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	The glycine cleavage system catalyzes the degradation of glycine. The P protein binds the alpha-amino group of glycine through its pyridoxal phosphate cofactor; CO2 is released and the remaining methylamine moiety is then transferred to the lipoamide cofactor of the H protein By similarity.
Catalytic activity	Glycine + H-protein-lipoyllysine = H-protein-S-aminomethyldihydrolipoyllysine + CO2. HAMAP MF_00712
Subunit structure	The glycine cleavage system is composed of four proteins: P, T, L and H. In this organism, the P 'protein' is an heterodimer of two subunits By similarity.
Sequence similarities	Belongs to the gcvP family. N-terminal subunit subfamily.

Ontologies

Keywords
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	glycine decarboxylation via glycine cleavage system Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Molecular function	glycine dehydrogenase (decarboxylating) activity Inferred from electronic annotation. Source: EC pyridoxal phosphate binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 444	444	Probable glycine dehydrogenase [decarboxylating] subunit 1 HAMAP MF_00712		PRO_1000045640

Sequences

	Sequence		Length	Mass (Da)
	Q3AET5-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: A9A0197BAEEB89D9	FASTA	444	48,908
	10 20 30 40 50 60 MKYTPHTPDE VREMLSSLGL SSIEELFSDI PEEVKLKRPL NLPSGMSELE VKKHLANLAA 70 80 90 100 110 120 KNGSADKYTV FLGAGVYDHY VPAVVNHILL RSEFYTAYTP YQAEMSQGVL QSIFEYQTMI 130 140 150 160 170 180 CELTGLDITN ASMYDGGSAL AEAALMAVSQ TRRDKVLVLA TVHPEYRSVV KTYTWGPEIE 190 200 210 220 230 240 VVEVPYKSGT VDLEKLEELI DDKTAAVLVQ HPNFFGQLEP VEEISRLIHA QKGLLVVAVD 250 260 270 280 290 300 PISLGILKPP AEYGADIAVG DGQALGNGLA FGGPHLGFFA ARKDLARRMP GRLVGLTTDK 310 320 330 340 350 360 EGNRGFVLTL QAREQHIRRE KATSNICSNQ ALNALAATVY LATVGKKGLK EIALQSLQKA 370 380 390 400 410 420 HYAFERLIGE GYEPLFSGPF FKEFVVKVKN EEEITQKLLK HHILAGPGIS RFYPELAPAL 430 440 MIAVTEKRTR EEIDNLVEVL GGDR

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000141 Genomic DNA. Translation: ABB15168.1.
RefSeq	YP_359349.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3727270.
GenomeReviews	Gene locus CHY_0491 in contig CP000141_GR.
KEGG	chy:CHY_0491.
NMPDR	fig|246194.3.peg.976.
TIGR	CHY_0491.
Phylogenomic databases
HOGENOM	Q3AET5.
OMA	Q3AET5. VANASMY.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_0491-MON.
Family and domain databases
HAMAP	MF_00712. [Tree]
InterPro	IPR003437. GDC-P. IPR015421. PyrdxlP-dep_Trfase_major_sub1. [Graphical view]
Gene3D	G3DSA:3.40.640.10. PyrdxlP-dep_Trfase_major_sub1. 1 hit.
PANTHER	PTHR11773. GDC-P. 1 hit.
Pfam	PF02347. GDC-P. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

GCSPA_CARHZ

Accession

Primary (citable) accession number: Q3AET5

Entry history

Integrated into UniProtKB/Swiss-Prot:	January 15, 2008
Last sequence update:	November 22, 2005
Last modified:	June 16, 2009
This is version 22 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)