Reviewed, UniProtKB/Swiss-Prot Q3AEQ2 (LEU3_CARHZ)

Names and origin

Protein names

Recommended name:
    3-isopropylmalate dehydrogenase
    EC=1.1.1.85
Alternative name(s):
    Beta-IPM dehydrogenase
      Short name=IMDH
    3-IPM-DH

Gene names

Name:	leuB
Ordered Locus Names:	CHY_0524

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	374 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Catalyzes the oxidation of 3-carboxy-2-hydroxy-4-methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2-oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate By similarity.
Catalytic activity	(2R,3S)-3-isopropylmalate + NAD(+) = 4-methyl-2-oxopentanoate + CO(2) + NADH.
Cofactor	Binds 1 magnesium or manganese ion per subunit By similarity.
Pathway	Amino-acid biosynthesis; L-leucine biosynthesis; L-leucine from 3-methyl-2-oxobutanoate: step 3/4.
Subunit structure	Homodimer By similarity.
Subcellular location	CytoplasmBy similarity.
Sequence similarities	Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily.

Ontologies

Keywords
Biological process	Amino-acid biosynthesis Branched-chain amino acid biosynthesis Leucine biosynthesis
Cellular component	Cytoplasm
Ligand	Magnesium Manganese Metal-binding NAD
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	leucine biosynthetic process Inferred from electronic annotation. Source: HAMAP oxidation reduction Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: HAMAP
Molecular function	3-isopropylmalate dehydrogenase activity Inferred from electronic annotation. Source: HAMAP magnesium ion binding Inferred from electronic annotation. Source: UniProtKB-KW manganese ion binding Inferred from electronic annotation. Source: UniProtKB-KW
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 374	374	3-isopropylmalate dehydrogenase		PRO_0000250110
Regions
	Nucleotide binding	83 – 96	14	NAD By similarity
	Nucleotide binding	288 – 300	13	NAD By similarity
Sites
	Metal binding	231	1	Magnesium or manganese By similarity
	Metal binding	255	1	Magnesium or manganese By similarity
	Metal binding	259	1	Magnesium or manganese By similarity
	Binding site	104	1	Substrate By similarity
	Binding site	114	1	Substrate By similarity
	Binding site	142	1	Substrate By similarity
	Binding site	231	1	Substrate By similarity
	Site	149	1	Important for catalysis By similarity
	Site	199	1	Important for catalysis By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AEQ2-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: FBA85D58E1DE8446	FASTA	374	40,975
	10 20 30 40 50 60 MSGRSCKGPF KILVLPGDGI GPEIIKEAVK VLKALGEKKG ITFNFQYGLI GGAAIDERGV 70 80 90 100 110 120 PLPEETVELG KQCDAILLGA VGGPKWDNLP PEIRPELGGL LKIRKVFDLY ANLRPVMFFP 130 140 150 160 170 180 ELKNASPLKP DIIEGVDILM VRELTGGLYF GEKKRFTTEQ GEQAVIDTLI YTEKEVERVV 190 200 210 220 230 240 RLGFELAQKR RGKLTLVDKA NVLESSRFWR EITGEIKKEY PDVELSYMYV DNCAMQLIRN 250 260 270 280 290 300 PRQFDVIVTE NMFGDILTDE GSVLAGSIGL LPSASLNGKF GLYEPIHGSA PDIAGQNKAN 310 320 330 340 350 360 PLATILSAGM MLRYSLDCPE EALLIEKAVK AVLQKGYRTG DILEPGTTLV TCEEMGDLVA 370 EEILGGVYDE GLPL

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000141 Genomic DNA. Translation: ABB15752.1.
RefSeq	YP_359382.1.
3D structure databases
ModBase	Search...
Genome annotation databases
GeneID	3727861.
GenomeReviews	Gene locus CHY_0524 in contig CP000141_GR.
KEGG	chy:CHY_0524.
NMPDR	fig|246194.3.peg.1091.
TIGR	CHY_0524.
Phylogenomic databases
HOGENOM	Q3AEQ2.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_0524-MON.
Family and domain databases
HAMAP	MF_01033. [Tree]
InterPro	IPR004429. 3-isopropylmalate_DHase. IPR001804. IsoCit_IM_DHase. [Graphical view]
Gene3D	G3DSA:3.40.718.10. IDH_IMDH. 1 hit.
PANTHER	PTHR11835. IDH_IMDH_dimeric. 1 hit. PTHR11835:SF13. IPMDH. 1 hit.
Pfam	PF00180. Iso_dh. 1 hit. [Graphical view]
TIGRFAMs	TIGR00169. leuB. 1 hit.
PROSITE	PS00470. IDH_IMDH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

LEU3_CARHZ

Accession

Primary (citable) accession number: Q3AEQ2

Entry history

Integrated into UniProtKB/Swiss-Prot:	September 19, 2006
Last sequence update:	November 22, 2005
Last modified:	November 4, 2008
This is version 25 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)