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Q3AEP2

- SYI_CARHZ

UniProt

Q3AEP2 - SYI_CARHZ

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Protein
Isoleucine--tRNA ligase
Gene
ileS, CHY_0534
Organism
Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Status
Reviewed - Annotation score: 3 out of 5 - Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile) By similarity.UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Binds 1 zinc ion per subunit By similarity.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylate By similarity
Binding sitei600 – 6001ATP By similarity
Metal bindingi890 – 8901Zinc By similarity
Metal bindingi893 – 8931Zinc By similarity
Metal bindingi910 – 9101Zinc By similarity
Metal bindingi913 – 9131Zinc By similarity

GO - Molecular functioni

  1. ATP binding Source: UniProtKB-HAMAP
  2. aminoacyl-tRNA editing activity Source: InterPro
  3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
  4. zinc ion binding Source: UniProtKB-HAMAP
Complete GO annotation...

GO - Biological processi

  1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP
Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligase (EC:6.1.1.5)
Alternative name(s):
Isoleucyl-tRNA synthetase
Short name:
IleRS
Gene namesi
Name:ileS
Ordered Locus Names:CHY_0534
OrganismiCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
ProteomesiUP000002706: Chromosome

Subcellular locationi

Cytoplasm By similarity UniRule annotation

GO - Cellular componenti

  1. cytoplasm Source: UniProtKB-SubCell
Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 925925Isoleucine--tRNA ligaseUniRule annotation
PRO_1000022056Add
BLAST

Interactioni

Subunit structurei

Monomer By similarity.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0534.

Structurei

3D structure databases

ProteinModelPortaliQ3AEP2.
SMRiQ3AEP2. Positions 1-920.

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionUniRule annotation
Add
BLAST
Motifi597 – 6015"KMSKS" regionUniRule annotation

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)) By similarity.UniRule annotation

Sequence similaritiesi

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiERLMLHQ.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3AEP2-1 [UniParc]FASTAAdd to Basket

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MDYGKTLNLP VTDFPMRGNL PEREPEILAY WQKIDLYRKV QQKNEGRPKF    50
ILHDGPPYAN GDIHMGHVLN KVLKDMIVKF KSMDGYDAPY VPGWDTHGLP 100
IEQRAIKDLG INRKEISPLE FRAKCREYAE KYARIQKEQF KRLGVRGDWE 150
NPYLTLMPHY EAKQIEIFGE MAKKGYIYKG LKPVYWCPVC ETALAEAEIE 200
YAEKKSPSIY VRFKVVEGKG KVPEDAYLVI WTTTPWTLPA NVAIAVHPEF 250
TYALVKTEKG DYVVAEGLVQ QFFEATKLTG EIVARFKGEE LLGVVTRHPF 300
IERESPVVLA DYVTLESGTG LVHTAPGHGT EDFETGKKYN LPVLSPVNHQ 350
GVFTEGAGKY AGMKIDDGNK AITQDLEASG DLLAMSFIKH SYPHCWRCKN 400
PVIFRATEQW FASIDGFREQ ALKEIEKVEW IPAWGKDRIY NMVRDRGDWC 450
ISRQRTWGVP IPIFYCEACG KEIITDESIK AVSDLFRVEG SDAWWKYEAG 500
EILPDGFRCP HCGGVKFRKE TDIMDVWFDS GSSHAAVLEQ PEYWPDLRWP 550
ADLYLEGSDQ HRGWFNSSLS TAVATRGMAP YKAVLTHGFL VDEKGRKMSK 600
SLGNVVDPLK VIKELGADIL RLWVASADYR SDLAISNNIL KQTAEGYRKI 650
RNTIRFLLGN LYDYDHEKHR VPYERLLEID RWALAKLHRL IARVVRAYRD 700
YEFHVVFHAV HNFCTVDMSA IYLDIIKDRL YVELPDSEKR RSAQTVLYEI 750
LDSLLRLLTP ILAFTTEEAY RHFPAKGKKE SVQLLDMPKV EERYLDLTLE 800
ETWDKILEVR AKVLKALEIA RQEKLIGHSL DAWVTLKASG ELYDFLVERV 850
NMWPEIFIVS QVDIQNEEVT GENGELPLEV VVNKALGEKC QRCWMYSPEV 900
GLDPEFPDTC PRCAGVLHEL KNRPL 925
Length:925
Mass (Da):106,237
Last modified:November 22, 2005 - v1
Checksum:iD5D5B6E014DD26D7
GO

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000141 Genomic DNA. Translation: ABB14741.1.
RefSeqiYP_359392.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB14741; ABB14741; CHY_0534.
GeneIDi3728472.
KEGGichy:CHY_0534.
PATRICi21274215. VBICarHyd26463_0495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBL
GenBank
DDBJ
Links Updated
CP000141 Genomic DNA. Translation: ABB14741.1 .
RefSeqi YP_359392.1. NC_007503.1.

3D structure databases

ProteinModelPortali Q3AEP2.
SMRi Q3AEP2. Positions 1-920.
ModBasei Search...

Protein-protein interaction databases

STRINGi 246194.CHY_0534.

Protocols and materials databases

Structural Biology Knowledgebase Search...

Genome annotation databases

EnsemblBacteriai ABB14741 ; ABB14741 ; CHY_0534 .
GeneIDi 3728472.
KEGGi chy:CHY_0534.
PATRICi 21274215. VBICarHyd26463_0495.

Phylogenomic databases

eggNOGi COG0060.
HOGENOMi HOG000246402.
KOi K01870.
OMAi ERLMLHQ.
OrthoDBi EOG644ZM1.

Enzyme and pathway databases

BioCyci CHYD246194:GJCN-535-MONOMER.

Family and domain databases

Gene3Di 1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPi MF_02002. Ile_tRNA_synth_type1.
InterProi IPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR013155. V/L/I-tRNA-synth_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view ]
PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
Pfami PF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view ]
PRINTSi PR00984. TRNASYNTHILE.
SUPFAMi SSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsi TIGR00392. ileS. 1 hit.
PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view ]
ProtoNeti Search...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: Z-2901 / DSM 6008.

Entry informationi

Entry nameiSYI_CARHZ
AccessioniPrimary (citable) accession number: Q3AEP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 14, 2014
This is version 65 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

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