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Q3AEP2

- SYI_CARHZ

UniProt

Q3AEP2 - SYI_CARHZ

Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
Status
Reviewed - Annotation score: 3 out of 5- Protein inferred from homologyi
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    • History
      Entry version 66 (01 Oct 2014)
      Sequence version 1 (22 Nov 2005)
      Previous versions | rss
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    Functioni

    Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

    Catalytic activityi

    ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

    Cofactori

    Binds 1 zinc ion per subunit.UniRule annotation

    Sites

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
    Binding sitei600 – 6001ATPUniRule annotation
    Metal bindingi890 – 8901ZincUniRule annotation
    Metal bindingi893 – 8931ZincUniRule annotation
    Metal bindingi910 – 9101ZincUniRule annotation
    Metal bindingi913 – 9131ZincUniRule annotation

    GO - Molecular functioni

    1. aminoacyl-tRNA editing activity Source: InterPro
    2. ATP binding Source: UniProtKB-HAMAP
    3. isoleucine-tRNA ligase activity Source: UniProtKB-HAMAP
    4. zinc ion binding Source: UniProtKB-HAMAP

    GO - Biological processi

    1. isoleucyl-tRNA aminoacylation Source: UniProtKB-HAMAP

    Keywords - Molecular functioni

    Aminoacyl-tRNA synthetase, Ligase

    Keywords - Biological processi

    Protein biosynthesis

    Keywords - Ligandi

    ATP-binding, Metal-binding, Nucleotide-binding, Zinc

    Enzyme and pathway databases

    BioCyciCHYD246194:GJCN-535-MONOMER.

    Names & Taxonomyi

    Protein namesi
    Recommended name:
    Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
    Alternative name(s):
    Isoleucyl-tRNA synthetaseUniRule annotation
    Short name:
    IleRSUniRule annotation
    Gene namesi
    Name:ileSUniRule annotation
    Ordered Locus Names:CHY_0534
    OrganismiCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008)
    Taxonomic identifieri246194 [NCBI]
    Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
    ProteomesiUP000002706: Chromosome

    Subcellular locationi

    Cytoplasm UniRule annotation

    GO - Cellular componenti

    1. cytoplasm Source: UniProtKB-SubCell

    Keywords - Cellular componenti

    Cytoplasm

    PTM / Processingi

    Molecule processing

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Chaini1 – 925925Isoleucine--tRNA ligasePRO_1000022056Add
    BLAST

    Interactioni

    Subunit structurei

    Monomer.UniRule annotation

    Protein-protein interaction databases

    STRINGi246194.CHY_0534.

    Structurei

    3D structure databases

    ProteinModelPortaliQ3AEP2.
    SMRiQ3AEP2. Positions 1-920.
    ModBaseiSearch...
    MobiDBiSearch...

    Family & Domainsi

    Motif

    Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
    Motifi57 – 6711"HIGH" regionAdd
    BLAST
    Motifi597 – 6015"KMSKS" region

    Domaini

    IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

    Sequence similaritiesi

    Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

    Phylogenomic databases

    eggNOGiCOG0060.
    HOGENOMiHOG000246402.
    KOiK01870.
    OMAiERLMLHQ.
    OrthoDBiEOG644ZM1.

    Family and domain databases

    Gene3Di1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPiMF_02002. Ile_tRNA_synth_type1.
    InterProiIPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view]
    PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
    PfamiPF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view]
    PRINTSiPR00984. TRNASYNTHILE.
    SUPFAMiSSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsiTIGR00392. ileS. 1 hit.
    PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view]

    Sequencei

    Sequence statusi: Complete.

    Q3AEP2-1 [UniParc]FASTAAdd to Basket

    « Hide

    MDYGKTLNLP VTDFPMRGNL PEREPEILAY WQKIDLYRKV QQKNEGRPKF    50
    ILHDGPPYAN GDIHMGHVLN KVLKDMIVKF KSMDGYDAPY VPGWDTHGLP 100
    IEQRAIKDLG INRKEISPLE FRAKCREYAE KYARIQKEQF KRLGVRGDWE 150
    NPYLTLMPHY EAKQIEIFGE MAKKGYIYKG LKPVYWCPVC ETALAEAEIE 200
    YAEKKSPSIY VRFKVVEGKG KVPEDAYLVI WTTTPWTLPA NVAIAVHPEF 250
    TYALVKTEKG DYVVAEGLVQ QFFEATKLTG EIVARFKGEE LLGVVTRHPF 300
    IERESPVVLA DYVTLESGTG LVHTAPGHGT EDFETGKKYN LPVLSPVNHQ 350
    GVFTEGAGKY AGMKIDDGNK AITQDLEASG DLLAMSFIKH SYPHCWRCKN 400
    PVIFRATEQW FASIDGFREQ ALKEIEKVEW IPAWGKDRIY NMVRDRGDWC 450
    ISRQRTWGVP IPIFYCEACG KEIITDESIK AVSDLFRVEG SDAWWKYEAG 500
    EILPDGFRCP HCGGVKFRKE TDIMDVWFDS GSSHAAVLEQ PEYWPDLRWP 550
    ADLYLEGSDQ HRGWFNSSLS TAVATRGMAP YKAVLTHGFL VDEKGRKMSK 600
    SLGNVVDPLK VIKELGADIL RLWVASADYR SDLAISNNIL KQTAEGYRKI 650
    RNTIRFLLGN LYDYDHEKHR VPYERLLEID RWALAKLHRL IARVVRAYRD 700
    YEFHVVFHAV HNFCTVDMSA IYLDIIKDRL YVELPDSEKR RSAQTVLYEI 750
    LDSLLRLLTP ILAFTTEEAY RHFPAKGKKE SVQLLDMPKV EERYLDLTLE 800
    ETWDKILEVR AKVLKALEIA RQEKLIGHSL DAWVTLKASG ELYDFLVERV 850
    NMWPEIFIVS QVDIQNEEVT GENGELPLEV VVNKALGEKC QRCWMYSPEV 900
    GLDPEFPDTC PRCAGVLHEL KNRPL 925
    Length:925
    Mass (Da):106,237
    Last modified:November 22, 2005 - v1
    Checksum:iD5D5B6E014DD26D7
    GO

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000141 Genomic DNA. Translation: ABB14741.1.
    RefSeqiYP_359392.1. NC_007503.1.

    Genome annotation databases

    EnsemblBacteriaiABB14741; ABB14741; CHY_0534.
    GeneIDi3728472.
    KEGGichy:CHY_0534.
    PATRICi21274215. VBICarHyd26463_0495.

    Cross-referencesi

    Sequence databases

    Select the link destinations:
    EMBL
    GenBank
    DDBJ
    Links Updated
    CP000141 Genomic DNA. Translation: ABB14741.1 .
    RefSeqi YP_359392.1. NC_007503.1.

    3D structure databases

    ProteinModelPortali Q3AEP2.
    SMRi Q3AEP2. Positions 1-920.
    ModBasei Search...
    MobiDBi Search...

    Protein-protein interaction databases

    STRINGi 246194.CHY_0534.

    Protocols and materials databases

    Structural Biology Knowledgebase Search...

    Genome annotation databases

    EnsemblBacteriai ABB14741 ; ABB14741 ; CHY_0534 .
    GeneIDi 3728472.
    KEGGi chy:CHY_0534.
    PATRICi 21274215. VBICarHyd26463_0495.

    Phylogenomic databases

    eggNOGi COG0060.
    HOGENOMi HOG000246402.
    KOi K01870.
    OMAi ERLMLHQ.
    OrthoDBi EOG644ZM1.

    Enzyme and pathway databases

    BioCyci CHYD246194:GJCN-535-MONOMER.

    Family and domain databases

    Gene3Di 1.10.730.10. 1 hit.
    3.40.50.620. 2 hits.
    3.90.740.10. 1 hit.
    HAMAPi MF_02002. Ile_tRNA_synth_type1.
    InterProi IPR001412. aa-tRNA-synth_I_CS.
    IPR002300. aa-tRNA-synth_Ia.
    IPR002301. Ile-tRNA-ligase.
    IPR023585. Ile-tRNA-ligase_type1.
    IPR014729. Rossmann-like_a/b/a_fold.
    IPR009080. tRNAsynth_1a_anticodon-bd.
    IPR013155. V/L/I-tRNA-synth_anticodon-bd.
    IPR009008. Val/Leu/Ile-tRNA-synth_edit.
    IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
    [Graphical view ]
    PANTHERi PTHR11946:SF9. PTHR11946:SF9. 1 hit.
    Pfami PF08264. Anticodon_1. 1 hit.
    PF00133. tRNA-synt_1. 1 hit.
    PF06827. zf-FPG_IleRS. 1 hit.
    [Graphical view ]
    PRINTSi PR00984. TRNASYNTHILE.
    SUPFAMi SSF47323. SSF47323. 1 hit.
    SSF50677. SSF50677. 1 hit.
    TIGRFAMsi TIGR00392. ileS. 1 hit.
    PROSITEi PS00178. AA_TRNA_LIGASE_I. 1 hit.
    [Graphical view ]
    ProtoNeti Search...

    Publicationsi

    1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
      Strain: Z-2901 / DSM 6008.

    Entry informationi

    Entry nameiSYI_CARHZ
    AccessioniPrimary (citable) accession number: Q3AEP2
    Entry historyi
    Integrated into UniProtKB/Swiss-Prot: January 15, 2008
    Last sequence update: November 22, 2005
    Last modified: October 1, 2014
    This is version 66 of the entry and version 1 of the sequence. [Complete history]
    Entry statusiReviewed (UniProtKB/Swiss-Prot)
    Annotation programProkaryotic Protein Annotation Program

    Miscellaneousi

    Keywords - Technical termi

    Complete proteome, Reference proteome

    Documents

    1. Aminoacyl-tRNA synthetases
      List of aminoacyl-tRNA synthetase entries
    2. SIMILARITY comments
      Index of protein domains and families

    External Data

    Dasty 3