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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Status
Reviewed-Annotation score: Annotation score: 3 out of 5-Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Binding sitei556 – 5561Aminoacyl-adenylateUniRule annotation
Binding sitei600 – 6001ATPUniRule annotation
Metal bindingi890 – 8901ZincUniRule annotation
Metal bindingi893 – 8931ZincUniRule annotation
Metal bindingi910 – 9101ZincUniRule annotation
Metal bindingi913 – 9131ZincUniRule annotation

GO - Molecular functioni

GO - Biological processi

Complete GO annotation...

Keywords - Molecular functioni

Aminoacyl-tRNA synthetase, Ligase

Keywords - Biological processi

Protein biosynthesis

Keywords - Ligandi

ATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-535-MONOMER.

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CHY_0534
OrganismiCarboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
ProteomesiUP000002706 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Complete GO annotation...

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Chaini1 – 925925Isoleucine--tRNA ligasePRO_1000022056Add
BLAST

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0534.

Structurei

3D structure databases

ProteinModelPortaliQ3AEP2.
SMRiQ3AEP2. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifierActions
Motifi57 – 6711"HIGH" regionAdd
BLAST
Motifi597 – 6015"KMSKS" region

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]

Sequencei

Sequence statusi: Complete.

Q3AEP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYGKTLNLP VTDFPMRGNL PEREPEILAY WQKIDLYRKV QQKNEGRPKF
60 70 80 90 100
ILHDGPPYAN GDIHMGHVLN KVLKDMIVKF KSMDGYDAPY VPGWDTHGLP
110 120 130 140 150
IEQRAIKDLG INRKEISPLE FRAKCREYAE KYARIQKEQF KRLGVRGDWE
160 170 180 190 200
NPYLTLMPHY EAKQIEIFGE MAKKGYIYKG LKPVYWCPVC ETALAEAEIE
210 220 230 240 250
YAEKKSPSIY VRFKVVEGKG KVPEDAYLVI WTTTPWTLPA NVAIAVHPEF
260 270 280 290 300
TYALVKTEKG DYVVAEGLVQ QFFEATKLTG EIVARFKGEE LLGVVTRHPF
310 320 330 340 350
IERESPVVLA DYVTLESGTG LVHTAPGHGT EDFETGKKYN LPVLSPVNHQ
360 370 380 390 400
GVFTEGAGKY AGMKIDDGNK AITQDLEASG DLLAMSFIKH SYPHCWRCKN
410 420 430 440 450
PVIFRATEQW FASIDGFREQ ALKEIEKVEW IPAWGKDRIY NMVRDRGDWC
460 470 480 490 500
ISRQRTWGVP IPIFYCEACG KEIITDESIK AVSDLFRVEG SDAWWKYEAG
510 520 530 540 550
EILPDGFRCP HCGGVKFRKE TDIMDVWFDS GSSHAAVLEQ PEYWPDLRWP
560 570 580 590 600
ADLYLEGSDQ HRGWFNSSLS TAVATRGMAP YKAVLTHGFL VDEKGRKMSK
610 620 630 640 650
SLGNVVDPLK VIKELGADIL RLWVASADYR SDLAISNNIL KQTAEGYRKI
660 670 680 690 700
RNTIRFLLGN LYDYDHEKHR VPYERLLEID RWALAKLHRL IARVVRAYRD
710 720 730 740 750
YEFHVVFHAV HNFCTVDMSA IYLDIIKDRL YVELPDSEKR RSAQTVLYEI
760 770 780 790 800
LDSLLRLLTP ILAFTTEEAY RHFPAKGKKE SVQLLDMPKV EERYLDLTLE
810 820 830 840 850
ETWDKILEVR AKVLKALEIA RQEKLIGHSL DAWVTLKASG ELYDFLVERV
860 870 880 890 900
NMWPEIFIVS QVDIQNEEVT GENGELPLEV VVNKALGEKC QRCWMYSPEV
910 920
GLDPEFPDTC PRCAGVLHEL KNRPL
Length:925
Mass (Da):106,237
Last modified:November 22, 2005 - v1
Checksum:iD5D5B6E014DD26D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14741.1.
RefSeqiWP_011343468.1. NC_007503.1.

Genome annotation databases

EnsemblBacteriaiABB14741; ABB14741; CHY_0534.
KEGGichy:CHY_0534.
PATRICi21274215. VBICarHyd26463_0495.

Cross-referencesi

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA. Translation: ABB14741.1.
RefSeqiWP_011343468.1. NC_007503.1.

3D structure databases

ProteinModelPortaliQ3AEP2.
SMRiQ3AEP2. Positions 1-920.
ModBaseiSearch...
MobiDBiSearch...

Protein-protein interaction databases

STRINGi246194.CHY_0534.

Protocols and materials databases

Structural Biology KnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaiABB14741; ABB14741; CHY_0534.
KEGGichy:CHY_0534.
PATRICi21274215. VBICarHyd26463_0495.

Phylogenomic databases

eggNOGiCOG0060.
HOGENOMiHOG000246402.
KOiK01870.
OMAiVLGDWDN.
OrthoDBiEOG644ZM1.

Enzyme and pathway databases

BioCyciCHYD246194:GJCN-535-MONOMER.

Family and domain databases

Gene3Di1.10.730.10. 1 hit.
3.40.50.620. 2 hits.
3.90.740.10. 1 hit.
HAMAPiMF_02002. Ile_tRNA_synth_type1.
InterProiIPR001412. aa-tRNA-synth_I_CS.
IPR002300. aa-tRNA-synth_Ia.
IPR002301. Ile-tRNA-ligase.
IPR023585. Ile-tRNA-ligase_type1.
IPR013155. M/V/L/I-tRNA-synth_anticd-bd.
IPR014729. Rossmann-like_a/b/a_fold.
IPR009080. tRNAsynth_1a_anticodon-bd.
IPR009008. Val/Leu/Ile-tRNA-synth_edit.
IPR010663. Znf_DNA_glyclase/IsotRNA_synth.
[Graphical view]
PANTHERiPTHR11946:SF9. PTHR11946:SF9. 1 hit.
PfamiPF08264. Anticodon_1. 1 hit.
PF00133. tRNA-synt_1. 1 hit.
PF06827. zf-FPG_IleRS. 1 hit.
[Graphical view]
PRINTSiPR00984. TRNASYNTHILE.
SUPFAMiSSF47323. SSF47323. 1 hit.
SSF50677. SSF50677. 1 hit.
TIGRFAMsiTIGR00392. ileS. 1 hit.
PROSITEiPS00178. AA_TRNA_LIGASE_I. 1 hit.
[Graphical view]
ProtoNetiSearch...

Publicationsi

  1. Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
    Strain: ATCC BAA-161 / DSM 6008 / Z-2901.

Entry informationi

Entry nameiSYI_CARHZ
AccessioniPrimary (citable) accession number: Q3AEP2
Entry historyi
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: July 22, 2015
This is version 73 of the entry and version 1 of the sequence. [Complete history]
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. Aminoacyl-tRNA synthetases
    List of aminoacyl-tRNA synthetase entries
  2. SIMILARITY comments
    Index of protein domains and families

External Data

Dasty 3

Similar proteinsi

Links to similar proteins from the UniProt Reference Clusters (UniRef) at 100%, 90% and 50% sequence identity:
100%UniRef100 combines identical sequences and sub-fragments with 11 or more residues from any organism into Uniref entry.
90%UniRef90 is built by clustering UniRef100 sequences that have at least 90% sequence identity to, and 80% overlap with, the longest sequence (a.k.a seed sequence).
50%UniRef50 is built by clustering UniRef90 seed sequences that have at least 50% sequence identity to, and 80% overlap with, the longest sequence in the cluster.