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Protein

Isoleucine--tRNA ligase

Gene

ileS

Organism
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Catalyzes the attachment of isoleucine to tRNA(Ile). As IleRS can inadvertently accommodate and process structurally similar amino acids such as valine, to avoid such errors it has two additional distinct tRNA(Ile)-dependent editing activities. One activity is designated as 'pretransfer' editing and involves the hydrolysis of activated Val-AMP. The other activity is designated 'posttransfer' editing and involves deacylation of mischarged Val-tRNA(Ile).UniRule annotation

Catalytic activityi

ATP + L-isoleucine + tRNA(Ile) = AMP + diphosphate + L-isoleucyl-tRNA(Ile).UniRule annotation

Cofactori

Zn2+UniRule annotationNote: Binds 1 zinc ion per subunit.UniRule annotation

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Binding sitei556Aminoacyl-adenylateUniRule annotation1
Binding sitei600ATPUniRule annotation1
Metal bindingi890ZincUniRule annotation1
Metal bindingi893ZincUniRule annotation1
Metal bindingi910ZincUniRule annotation1
Metal bindingi913ZincUniRule annotation1

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionAminoacyl-tRNA synthetase, Ligase
Biological processProtein biosynthesis
LigandATP-binding, Metal-binding, Nucleotide-binding, Zinc

Enzyme and pathway databases

BioCyciCHYD246194:G1G5B-483-MONOMER

Names & Taxonomyi

Protein namesi
Recommended name:
Isoleucine--tRNA ligaseUniRule annotation (EC:6.1.1.5UniRule annotation)
Alternative name(s):
Isoleucyl-tRNA synthetaseUniRule annotation
Short name:
IleRSUniRule annotation
Gene namesi
Name:ileSUniRule annotation
Ordered Locus Names:CHY_0534
OrganismiCarboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
Proteomesi
  • UP000002706 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000220561 – 925Isoleucine--tRNA ligaseAdd BLAST925

Proteomic databases

PRIDEiQ3AEP2

Interactioni

Subunit structurei

Monomer.UniRule annotation

Protein-protein interaction databases

STRINGi246194.CHY_0534

Structurei

3D structure databases

ProteinModelPortaliQ3AEP2
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Motif

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Motifi57 – 67"HIGH" regionAdd BLAST11
Motifi597 – 601"KMSKS" region5

Domaini

IleRS has two distinct active sites: one for aminoacylation and one for editing. The misactivated valine is translocated from the active site to the editing site, which sterically excludes the correctly activated isoleucine. The single editing site contains two valyl binding pockets, one specific for each substrate (Val-AMP or Val-tRNA(Ile)).UniRule annotation

Sequence similaritiesi

Belongs to the class-I aminoacyl-tRNA synthetase family. IleS type 1 subfamily.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105C07 Bacteria
COG0060 LUCA
HOGENOMiHOG000246402
KOiK01870
OMAiHLGTAWN
OrthoDBiPOG091H028I

Family and domain databases

CDDicd07960 Anticodon_Ia_Ile_BEm, 1 hit
Gene3Di3.40.50.620, 2 hits
3.90.740.10, 1 hit
HAMAPiMF_02002 Ile_tRNA_synth_type1, 1 hit
InterProiView protein in InterPro
IPR001412 aa-tRNA-synth_I_CS
IPR002300 aa-tRNA-synth_Ia
IPR033708 Anticodon_Ile_BEm
IPR002301 Ile-tRNA-ligase
IPR023585 Ile-tRNA-ligase_type1
IPR013155 M/V/L/I-tRNA-synth_anticd-bd
IPR014729 Rossmann-like_a/b/a_fold
IPR009080 tRNAsynth_Ia_anticodon-bd
IPR009008 Val/Leu/Ile-tRNA-synth_edit
IPR010663 Znf_FPG/IleRS
PfamiView protein in Pfam
PF08264 Anticodon_1, 1 hit
PF00133 tRNA-synt_1, 1 hit
PF06827 zf-FPG_IleRS, 1 hit
PRINTSiPR00984 TRNASYNTHILE
SUPFAMiSSF47323 SSF47323, 1 hit
SSF50677 SSF50677, 1 hit
TIGRFAMsiTIGR00392 ileS, 1 hit
PROSITEiView protein in PROSITE
PS00178 AA_TRNA_LIGASE_I, 1 hit

Sequencei

Sequence statusi: Complete.

Q3AEP2-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MDYGKTLNLP VTDFPMRGNL PEREPEILAY WQKIDLYRKV QQKNEGRPKF
60 70 80 90 100
ILHDGPPYAN GDIHMGHVLN KVLKDMIVKF KSMDGYDAPY VPGWDTHGLP
110 120 130 140 150
IEQRAIKDLG INRKEISPLE FRAKCREYAE KYARIQKEQF KRLGVRGDWE
160 170 180 190 200
NPYLTLMPHY EAKQIEIFGE MAKKGYIYKG LKPVYWCPVC ETALAEAEIE
210 220 230 240 250
YAEKKSPSIY VRFKVVEGKG KVPEDAYLVI WTTTPWTLPA NVAIAVHPEF
260 270 280 290 300
TYALVKTEKG DYVVAEGLVQ QFFEATKLTG EIVARFKGEE LLGVVTRHPF
310 320 330 340 350
IERESPVVLA DYVTLESGTG LVHTAPGHGT EDFETGKKYN LPVLSPVNHQ
360 370 380 390 400
GVFTEGAGKY AGMKIDDGNK AITQDLEASG DLLAMSFIKH SYPHCWRCKN
410 420 430 440 450
PVIFRATEQW FASIDGFREQ ALKEIEKVEW IPAWGKDRIY NMVRDRGDWC
460 470 480 490 500
ISRQRTWGVP IPIFYCEACG KEIITDESIK AVSDLFRVEG SDAWWKYEAG
510 520 530 540 550
EILPDGFRCP HCGGVKFRKE TDIMDVWFDS GSSHAAVLEQ PEYWPDLRWP
560 570 580 590 600
ADLYLEGSDQ HRGWFNSSLS TAVATRGMAP YKAVLTHGFL VDEKGRKMSK
610 620 630 640 650
SLGNVVDPLK VIKELGADIL RLWVASADYR SDLAISNNIL KQTAEGYRKI
660 670 680 690 700
RNTIRFLLGN LYDYDHEKHR VPYERLLEID RWALAKLHRL IARVVRAYRD
710 720 730 740 750
YEFHVVFHAV HNFCTVDMSA IYLDIIKDRL YVELPDSEKR RSAQTVLYEI
760 770 780 790 800
LDSLLRLLTP ILAFTTEEAY RHFPAKGKKE SVQLLDMPKV EERYLDLTLE
810 820 830 840 850
ETWDKILEVR AKVLKALEIA RQEKLIGHSL DAWVTLKASG ELYDFLVERV
860 870 880 890 900
NMWPEIFIVS QVDIQNEEVT GENGELPLEV VVNKALGEKC QRCWMYSPEV
910 920
GLDPEFPDTC PRCAGVLHEL KNRPL
Length:925
Mass (Da):106,237
Last modified:November 22, 2005 - v1
Checksum:iD5D5B6E014DD26D7
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA Translation: ABB14741.1
RefSeqiWP_011343468.1, NC_007503.1

Genome annotation databases

EnsemblBacteriaiABB14741; ABB14741; CHY_0534
KEGGichy:CHY_0534

Similar proteinsi

Entry informationi

Entry nameiSYI_CARHZ
AccessioniPrimary (citable) accession number: Q3AEP2
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 91 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

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