Reviewed, UniProtKB/Swiss-Prot Q3ADG9 (CHEB1_CARHZ)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 1
    EC=3.1.1.61

Gene names

Name:	cheB1
Ordered Locus Names:	CHY_0968

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity. HAMAP MF_00099
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm HAMAP MF_00099.
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity. HAMAP MF_00099
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Chemotaxis response regulator protein-glutamate methylesterase 1 HAMAP MF_00099		PRO_0000225452
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	148 – 340	193	CheB-type methylesterase
Sites
	Active site	160	1	By similarity
	Active site	187	1	By similarity
	Active site	285	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ADG9-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: E613699C343E4340	FASTA	340	36,528
	10 20 30 40 50 60 MSRYKLFIVD DSALIRLRLK TSLALWPEIQ IVGEAENGQE ALKKIPLVKP DVVTLDLEMP 70 80 90 100 110 120 VLDGLSTLKE LNKVYPVPVI MLSSLTTHGA KATIEALENG AVDFVPKDGD WNKVVSELKE 130 140 150 160 170 180 KIKIAVLAKK RPKPFTGGTL NPKNVILGKN GRQLVVIGAS TGGPPALREI IPKLPQTFPV 190 200 210 220 230 240 PIVIIQHITR GFSKPLADQL ARVSRLKVKE AEKDEQLLPG TVYVAPAGYT FKIDKQGGSL 250 260 270 280 290 300 TAKIIEPVEY LPAHFYPSVD EAMLSAAEVT GSKTIGVLLT GMGKDGALGM KAIKERGGYT 310 320 330 340 IAQDEETSVV YGMPKAAIDI GGVSRVLPLS AIAEEIAANI

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB14452.1.
RefSeq	YP_359815.1.
3D structure databases
SMR	Q3ADG9. Positions 1-339.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ADG9.
Genome annotation databases
GeneID	3728901.
GenomeReviews	Gene locus CHY_0968 in contig CP000141_GR.
KEGG	chy:CHY_0968.
NMPDR	fig|246194.3.peg.445.
TIGR	CHY_0968.
Phylogenomic databases
eggNOG	COG2201.
HOGENOM	HBG705324.
OMA	HTHEGAR.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_0968-MONOMER.
Family and domain databases
HAMAP	MF_00099. CheB_methylest. [Tree]
InterPro	IPR011006. CheY-like. IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB1_CARHZ

Accession

Primary (citable) accession number: Q3ADG9

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	January 19, 2010
This is version 32 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)