Reviewed, UniProtKB/Swiss-Prot Q3ADA6 (CHEB2_CARHZ)

Names and origin

Protein names

Recommended name:
    Chemotaxis response regulator protein-glutamate methylesterase 2
    EC=3.1.1.61

Gene names

Name:	cheB2
Ordered Locus Names:	CHY_1031

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	354 AA.
Sequence status	Complete.
Sequence processing	The displayed sequence is not processed.
Protein existence	Inferred from homology.

General annotation (Comments)

Function	Involved in the modulation of the chemotaxis system; catalyzes the demethylation of specific methylglutamate residues introduced into the chemoreceptors (methyl-accepting chemotaxis proteins) by cheR By similarity.
Catalytic activity	Protein L-glutamate O(5)-methyl ester + H2O = protein L-glutamate + methanol. HAMAP MF_00099
Subcellular location	Cytoplasm. HAMAP MF_00099
Domain	The N-terminal regulatory domain inhibits the activity of the C-terminal effector domain. HAMAP MF_00099
Post-translational modification	Phosphorylated by cheA. Phosphorylation suppresses the inhibitory activity of the N-terminal domain By similarity.
Sequence similarities	Contains 1 cheB-type methylesterase domain. Contains 1 response regulatory domain.

Ontologies

Keywords
Biological process	Chemotaxis
Cellular component	Cytoplasm
Molecular function	Hydrolase
PTM	Phosphoprotein
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	chemotaxis Inferred from electronic annotation. Source: HAMAP regulation of transcription, DNA-dependent Inferred from electronic annotation. Source: InterPro sensory perception of chemical stimulus Inferred from electronic annotation. Source: HAMAP two-component signal transduction system (phosphorelay) Inferred from electronic annotation. Source: HAMAP
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	protein-glutamate methylesterase activity Inferred from electronic annotation. Source: HAMAP two-component response regulator activity Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 354	354	Chemotaxis response regulator protein-glutamate methylesterase 2 HAMAP MF_00099		PRO_0000225453
Regions
	Domain	5 – 122	118	Response regulatory
	Domain	159 – 354	196	CheB-type methylesterase
Sites
	Active site	172	1	By similarity
	Active site	199	1	By similarity
	Active site	298	1	By similarity
Amino acid modifications
	Modified residue	56	1	4-aspartylphosphate By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ADA6-1 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 03D2A43384D2D797	FASTA	354	39,156
	10 20 30 40 50 60 MSKIRVLIVD DSALMRKYLR EILEQDPEIE VIAVARNGRE AVEKALELSP DVITMDINMP 70 80 90 100 110 120 EMDGLTAIQY IMLHRPTPII VISSLTQKGA LTTFEALELG AVDYVAKPDG TVSLKIKEVA 130 140 150 160 170 180 EEILQKVKAV AKSRGVVRLK ARKERPFVQE VPKTRPVSPD TSFKKLILIG VSTGGPKALM 190 200 210 220 230 240 EIIPRLPGNL DASVVIIQHM PEKFTASFAA RLNNYSELYV KEAEDGESLE RGKVLVARGG 250 260 270 280 290 300 INLKLERKLG INVVRVRYTP LPRETIYWPS VDVAFRSALT VIEPHRIIAV LLTGMGDDGA 310 320 330 340 350 QAMVEIKQKG GYTIAESEET AVVWGMPREA IERGGASEIL PVYQIADRIV ELVR

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].

Cross-references

Sequence databases
EMBLGenBankDDBJ	CP000141 Genomic DNA. Translation: ABB14613.1.
RefSeq	YP_359878.1.
3D structure databases
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ADA6.
Genome annotation databases
GeneID	3728752.
GenomeReviews	Gene locus CHY_1031 in contig CP000141_GR.
KEGG	chy:CHY_1031.
NMPDR	fig|246194.3.peg.785.
TIGR	CHY_1031.
Phylogenomic databases
HOGENOM	Q3ADA6.
OMA	THEGAIT.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_1031-MON.
Family and domain databases
HAMAP	MF_00099. [Tree]
InterPro	IPR008248. Sig_transdc_resp-reg_CheB. IPR000673. Sig_transdc_resp-reg_Me-estase. IPR001789. Sig_transdc_resp-reg_receiver. [Graphical view]
Gene3D	G3DSA:3.40.50.180. Chemotax_RR_pGlu_Me-esterase. 1 hit.
Pfam	PF01339. CheB_methylest. 1 hit. PF00072. Response_reg. 1 hit. [Graphical view]
PIRSF	PIRSF000876. RR_chemtxs_CheB. 1 hit.
ProDom	PD005328. CheB_methylest. 1 hit. PD000039. Response_reg. 1 hit. [Graphical view] [Entries sharing at least one domain]
SMART	SM00448. REC. 1 hit. [Graphical view]
PROSITE	PS50122. CHEB. 1 hit. PS50110. RESPONSE_REGULATORY. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

CHEB2_CARHZ

Accession

Primary (citable) accession number: Q3ADA6

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	November 3, 2009
This is version 29 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation project

HAMAP (High-quality Automated and Manual Annotation of microbial Proteomes)