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Q3ACF5 (DDL_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Last modified February 19, 2014. Version 62. Feed History...

Clusters with 100%, 90%, 50% identity | Documents (2) | Third-party data text xml rdf/xml gff fasta
to top of pageNames·Attributes·General annotation·Ontologies·Sequence annotation·Sequences·References·Cross-refs·Entry info·DocumentsCustomize order

Names and origin

Protein namesRecommended name:
D-alanine--D-alanine ligase

EC=6.3.2.4
Alternative name(s):
D-Ala-D-Ala ligase
D-alanylalanine synthetase
Gene names
Name:ddl
Ordered Locus Names:CHY_1346
OrganismCarboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]
Taxonomic identifier246194 [NCBI]
Taxonomic lineageBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus

Protein attributes

Sequence length312 AA.
Sequence statusComplete.
Protein existenceInferred from homology

General annotation (Comments)

Function

Cell wall formation By similarity. HAMAP-Rule MF_00047

Catalytic activity

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine. HAMAP-Rule MF_00047

Cofactor

Binds 2 magnesium or manganese ions per subunit By similarity.

Pathway

Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP-Rule MF_00047

Subcellular location

Cytoplasm By similarity HAMAP-Rule MF_00047.

Sequence similarities

Belongs to the D-alanine--D-alanine ligase family.

Contains 1 ATP-grasp domain.

Sequence annotation (Features)

Feature keyPosition(s)LengthDescriptionGraphical viewFeature identifier

Molecule processing

Chain1 – 312312D-alanine--D-alanine ligase HAMAP-Rule MF_00047
PRO_1000030435

Regions

Domain99 – 304206ATP-grasp
Nucleotide binding131 – 18656ATP By similarity

Sites

Metal binding2571Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 1 By similarity
Metal binding2711Magnesium or manganese 2 By similarity
Metal binding2731Magnesium or manganese 2 By similarity

Sequences

Sequence LengthMass (Da)Tools
Q3ACF5 [UniParc].

Last modified November 22, 2005. Version 1.
Checksum: B407789057080FF8

FASTA31234,462
        10         20         30         40         50         60 
MKIGVICGGL SSEREVSLRT GDAIFRALLK KGYNVVKIDM DRNIAETLKK ENIDFAFIAL 

        70         80         90        100        110        120 
HGKYGEDGAI QGLLEIMDIP YTGSGILASS LAIDKIMTKK ILKAEGIPTP NYIAFSFDEN 

       130        140        150        160        170        180 
PNFEAISSEI LQTLKLPVVI KAPREGSTIG IEFVFSKQEL PKAIKKVLEI DKQLLVEEFI 

       190        200        210        220        230        240 
EGVEVTASVL GNSNPVVLPL IEIVSKTRFY DYEAKYTPGL SEHIIPPRIA PELSQKAIEY 

       250        260        270        280        290        300 
ARKTYKALGC RGFARVDFMI DVRKNEAYVL EVNTIPGMTA TSLFPDAAKA QGISFEDLVE 

       310 
KILMLGLEGR QK 

« Hide

References

[1]"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901."
Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A.
PLoS Genet. 1:563-574(2005) [PubMed] [Europe PMC] [Abstract]
Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA].
Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases

EMBL
GenBank
DDBJ
CP000141 Genomic DNA. Translation: ABB14185.1.
RefSeqYP_360179.1. NC_007503.1.

3D structure databases

ProteinModelPortalQ3ACF5.
ModBaseSearch...
MobiDBSearch...

Protein-protein interaction databases

STRING246194.CHY_1346.

Protocols and materials databases

StructuralBiologyKnowledgebaseSearch...

Genome annotation databases

EnsemblBacteriaABB14185; ABB14185; CHY_1346.
GeneID3728690.
KEGGchy:CHY_1346.
PATRIC21275827. VBICarHyd26463_1288.

Organism-specific databases

CMRSearch...

Phylogenomic databases

eggNOGCOG1181.
HOGENOMHOG000011592.
KOK01921.
OMAKYTEGFA.
OrthoDBEOG6ND0KB.
ProtClustDBPRK01372.

Enzyme and pathway databases

BioCycCHYD246194:GJCN-1345-MONOMER.
UniPathwayUPA00219.

Family and domain databases

Gene3D3.30.1490.20. 1 hit.
3.30.470.20. 2 hits.
3.40.50.20. 1 hit.
HAMAPMF_00047. Dala_Dala_lig.
InterProIPR011761. ATP-grasp.
IPR013815. ATP_grasp_subdomain_1.
IPR013816. ATP_grasp_subdomain_2.
IPR000291. D-Ala_lig_Van_CS.
IPR005905. D_ala_D_ala.
IPR011095. Dala_Dala_lig_C.
IPR011127. Dala_Dala_lig_N.
IPR016185. PreATP-grasp_dom.
[Graphical view]
PANTHERPTHR23132. PTHR23132. 1 hit.
PfamPF07478. Dala_Dala_lig_C. 1 hit.
PF01820. Dala_Dala_lig_N. 2 hits.
[Graphical view]
SUPFAMSSF52440. SSF52440. 1 hit.
TIGRFAMsTIGR01205. D_ala_D_alaTIGR. 1 hit.
PROSITEPS50975. ATP_GRASP. 1 hit.
PS00843. DALA_DALA_LIGASE_1. 1 hit.
PS00844. DALA_DALA_LIGASE_2. 1 hit.
[Graphical view]
ProtoNetSearch...

Entry information

Entry nameDDL_CARHZ
AccessionPrimary (citable) accession number: Q3ACF5
Entry history
Integrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: February 19, 2014
This is version 62 of the entry and version 1 of the sequence. [Complete history]
Entry statusReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Relevant documents

SIMILARITY comments

Index of protein domains and families

PATHWAY comments

Index of metabolic and biosynthesis pathways