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Protein

D-alanine--D-alanine ligase

Gene

ddl

Organism
Carboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Status
Reviewed-Annotation score: -Protein inferred from homologyi

Functioni

Cell wall formation.UniRule annotation

Catalytic activityi

ATP + 2 D-alanine = ADP + phosphate + D-alanyl-D-alanine.UniRule annotation

Cofactori

Mg2+By similarity, Mn2+By similarityNote: Binds 2 magnesium or manganese ions per subunit.By similarity

Pathwayi: peptidoglycan biosynthesis

This protein is involved in the pathway peptidoglycan biosynthesis, which is part of Cell wall biogenesis.UniRule annotation
View all proteins of this organism that are known to be involved in the pathway peptidoglycan biosynthesis and in Cell wall biogenesis.

Sites

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Metal bindingi257Magnesium or manganese 1UniRule annotation1
Metal bindingi271Magnesium or manganese 1UniRule annotation1
Metal bindingi271Magnesium or manganese 2UniRule annotation1
Metal bindingi273Magnesium or manganese 2UniRule annotation1

Regions

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Nucleotide bindingi131 – 186ATPUniRule annotationAdd BLAST56

GO - Molecular functioni

GO - Biological processi

Keywordsi

Molecular functionLigase
Biological processCell shape, Cell wall biogenesis/degradation, Peptidoglycan synthesis
LigandATP-binding, Magnesium, Manganese, Metal-binding, Nucleotide-binding

Enzyme and pathway databases

BioCyciCHYD246194:G1G5B-1252-MONOMER
UniPathwayiUPA00219

Names & Taxonomyi

Protein namesi
Recommended name:
D-alanine--D-alanine ligaseUniRule annotation (EC:6.3.2.4UniRule annotation)
Alternative name(s):
D-Ala-D-Ala ligaseUniRule annotation
D-alanylalanine synthetaseUniRule annotation
Gene namesi
Name:ddlUniRule annotation
Ordered Locus Names:CHY_1346
OrganismiCarboxydothermus hydrogenoformans (strain ATCC BAA-161 / DSM 6008 / Z-2901)
Taxonomic identifieri246194 [NCBI]
Taxonomic lineageiBacteriaFirmicutesClostridiaThermoanaerobacteralesThermoanaerobacteraceaeCarboxydothermus
Proteomesi
  • UP000002706 Componenti: Chromosome

Subcellular locationi

  • Cytoplasm UniRule annotation

GO - Cellular componenti

Keywords - Cellular componenti

Cytoplasm

PTM / Processingi

Molecule processing

Feature keyPosition(s)DescriptionActionsGraphical viewLength
ChainiPRO_10000304351 – 312D-alanine--D-alanine ligaseAdd BLAST312

Proteomic databases

PRIDEiQ3ACF5

Interactioni

Protein-protein interaction databases

STRINGi246194.CHY_1346

Structurei

3D structure databases

ProteinModelPortaliQ3ACF5
SMRiQ3ACF5
ModBaseiSearch...
MobiDBiSearch...

Family & Domainsi

Domains and Repeats

Feature keyPosition(s)DescriptionActionsGraphical viewLength
Domaini99 – 304ATP-graspUniRule annotationAdd BLAST206

Sequence similaritiesi

Belongs to the D-alanine--D-alanine ligase family.UniRule annotation

Phylogenomic databases

eggNOGiENOG4105CPF Bacteria
COG1181 LUCA
HOGENOMiHOG000011592
KOiK01921
OMAiMQGLLEC
OrthoDBiPOG091H00GT

Family and domain databases

Gene3Di3.30.1490.20, 1 hit
HAMAPiMF_00047 Dala_Dala_lig, 1 hit
InterProiView protein in InterPro
IPR011761 ATP-grasp
IPR013815 ATP_grasp_subdomain_1
IPR000291 D-Ala_lig_Van_CS
IPR005905 D_ala_D_ala
IPR011095 Dala_Dala_lig_C
IPR011127 Dala_Dala_lig_N
IPR016185 PreATP-grasp_dom_sf
PfamiView protein in Pfam
PF07478 Dala_Dala_lig_C, 1 hit
PF01820 Dala_Dala_lig_N, 1 hit
PIRSFiPIRSF039102 Ddl/VanB, 1 hit
SUPFAMiSSF52440 SSF52440, 1 hit
TIGRFAMsiTIGR01205 D_ala_D_alaTIGR, 1 hit
PROSITEiView protein in PROSITE
PS50975 ATP_GRASP, 1 hit
PS00843 DALA_DALA_LIGASE_1, 1 hit
PS00844 DALA_DALA_LIGASE_2, 1 hit

Sequencei

Sequence statusi: Complete.

Q3ACF5-1 [UniParc]FASTAAdd to basket

« Hide

        10         20         30         40         50
MKIGVICGGL SSEREVSLRT GDAIFRALLK KGYNVVKIDM DRNIAETLKK
60 70 80 90 100
ENIDFAFIAL HGKYGEDGAI QGLLEIMDIP YTGSGILASS LAIDKIMTKK
110 120 130 140 150
ILKAEGIPTP NYIAFSFDEN PNFEAISSEI LQTLKLPVVI KAPREGSTIG
160 170 180 190 200
IEFVFSKQEL PKAIKKVLEI DKQLLVEEFI EGVEVTASVL GNSNPVVLPL
210 220 230 240 250
IEIVSKTRFY DYEAKYTPGL SEHIIPPRIA PELSQKAIEY ARKTYKALGC
260 270 280 290 300
RGFARVDFMI DVRKNEAYVL EVNTIPGMTA TSLFPDAAKA QGISFEDLVE
310
KILMLGLEGR QK
Length:312
Mass (Da):34,462
Last modified:November 22, 2005 - v1
Checksum:iB407789057080FF8
GO

Sequence databases

Select the link destinations:
EMBLi
GenBanki
DDBJi
Links Updated
CP000141 Genomic DNA Translation: ABB14185.1
RefSeqiWP_011344255.1, NC_007503.1

Genome annotation databases

EnsemblBacteriaiABB14185; ABB14185; CHY_1346
KEGGichy:CHY_1346

Similar proteinsi

Entry informationi

Entry nameiDDL_CARHZ
AccessioniPrimary (citable) accession number: Q3ACF5
Entry historyiIntegrated into UniProtKB/Swiss-Prot: January 15, 2008
Last sequence update: November 22, 2005
Last modified: May 23, 2018
This is version 87 of the entry and version 1 of the sequence. See complete history.
Entry statusiReviewed (UniProtKB/Swiss-Prot)
Annotation programProkaryotic Protein Annotation Program

Miscellaneousi

Keywords - Technical termi

Complete proteome, Reference proteome

Documents

  1. PATHWAY comments
    Index of metabolic and biosynthesis pathways
  2. SIMILARITY comments
    Index of protein domains and families

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