Q3ABX6 (NADE_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
NH(3)-dependent NAD(+) synthetase
EC=6.3.1.5

Gene names

Name:	nadE
Ordered Locus Names:	CHY_1526

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	243 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + deamido-NAD+ + NH3 = AMP + diphosphate + NAD+. HAMAP MF_00193
Pathway	Cofactor biosynthesis; NAD(+) biosynthesis; NAD(+) from deamido-NAD(+) (ammonia route): step 1/1. HAMAP MF_00193
Sequence similarities	Belongs to the NAD synthetase family.

Ontologies

Keywords
Ligand	ATP-binding NAD Nucleotide-binding
Molecular function	Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	NAD biosynthetic process Inferred from electronic annotation. Source: InterPro
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW NAD+ synthase (glutamine-hydrolyzing) activity Inferred from electronic annotation. Source: InterPro NAD+ synthase activity Inferred from electronic annotation. Source: EC
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 243	243	NH(3)-dependent NAD(+) synthetase HAMAP MF_00193		PRO_1000077544
Regions
	Nucleotide binding	31 – 38	8	ATP By similarity
Sites
	Active site	33	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ABX6 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: 0B5F5C45BC894595	FASTA	243	27,369
	10 20 30 40 50 60 MRVNWEEKTE KLVNWLREKT REANASGLLV GLSGGVDSAV VATLIKKAFP EKSLGIIMPC 70 80 90 100 110 120 FSNPEDEEDA RMIANHLNLK YIVVNLDEPY QALVSSLKNA TPHEPEKLAL ANIKPRLRMT 130 140 150 160 170 180 TLYYWAANLN YLVAGTGNRT ELEIGYFTKW GDGGVDLLPI GNLTKTEVWE FARYLGLPEK 190 200 210 220 230 240 IITKAPSAGL WEGQTDEGEM GFTYKDIDHY LLTGEGSAEL VDFVKRMQRK SQHKKRIPEV PML

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB13788.1.
RefSeq	YP_360358.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3ABX6.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ABX6.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3726907.
GenomeReviews	Gene locus CHY_1526 in contig CP000141_GR.
KEGG	chy:CHY_1526.
NMPDR	fig|246194.3.peg.1486.
PATRIC	21276181. VBICarHyd26463_1465.
TIGR	CHY_1526.
Phylogenomic databases
eggNOG	COG0171.
HOGENOM	HBG351567.
OMA	YDISARD.
ProtClustDB	CLSK717777.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_1526-MONOMER.
Family and domain databases
HAMAP	MF_00193. NadE. [Tree]
InterPro	IPR022310. NAD/GMP_synthase. IPR003694. NAD_synthase. IPR022926. NH(3)-dep_NAD(+)_synth. IPR014729. Rossmann-like_a/b/a_fold. [Graphical view]
Gene3D	G3DSA:3.40.50.620. Rossmann-like_a/b/a_fold. 1 hit.
KO	K01916.
Pfam	PF02540. NAD_synthase. 1 hit. [Graphical view]
TIGRFAMs	TIGR00552. NadE. 1 hit.
ProtoNet	Search...

Entry information

Entry name

NADE_CARHZ

Accession

Primary (citable) accession number: Q3ABX6

Entry history

Integrated into UniProtKB/Swiss-Prot:	May 20, 2008
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program