Q3ABT4 (SYFA_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
Phenylalanine--tRNA ligase alpha chain
EC=6.1.1.20

Alternative name(s):
Phenylalanyl-tRNA synthetase alpha chain
Short name=PheRS

Gene names

Name:	pheS
Ordered Locus Names:	CHY_1571

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	340 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Catalytic activity	ATP + L-phenylalanine + tRNA(Phe) = AMP + diphosphate + L-phenylalanyl-tRNA(Phe). HAMAP MF_00281
Cofactor	Binds 2 magnesium ions per tetramer By similarity. HAMAP MF_00281
Subunit structure	Tetramer of two alpha and two beta chains By similarity.
Subcellular location	Cytoplasm HAMAP MF_00281.
Sequence similarities	Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily.

Ontologies

Keywords
Biological process	Protein biosynthesis
Cellular component	Cytoplasm
Ligand	ATP-binding Magnesium Metal-binding Nucleotide-binding
Molecular function	Aminoacyl-tRNA synthetase Ligase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	phenylalanyl-tRNA aminoacylation Inferred from electronic annotation. Source: InterPro
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	ATP binding Inferred from electronic annotation. Source: UniProtKB-KW metal ion binding Inferred from electronic annotation. Source: UniProtKB-KW phenylalanine-tRNA ligase activity Inferred from electronic annotation. Source: EC tRNA binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 340	340	Phenylalanine--tRNA ligase alpha chain HAMAP MF_00281		PRO_0000231971
Sites
	Metal binding	255	1	Magnesium By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3ABT4 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: AA764A26ABFF25CF	FASTA	340	38,640
	10 20 30 40 50 60 MNLHNEELWQ KYLGEVIRVS DLNELQTVKV KFLGKKGEVT LALKNLKNLP PELRPEAGHK 70 80 90 100 110 120 INTVKKLIEE DLEKREQELL QKALEEKLSK EAIDVTLPGY SLKLGKLHPL TQVQHRIVEI 130 140 150 160 170 180 FTSMGFSVAT GPEIEKDYYN FEALNLPKDH PARDMQDSFY ITEEVLLRTH TSPVQVRVME 190 200 210 220 230 240 KYAPNLPIRI ISPGKVYRRD DDATHSPMFA QCEGLAVDKN IRFSDLKGVL LTFIKELFGP 250 260 270 280 290 300 KTKMRLRPSY FPFTEPSAEV DISCVICGGI GCKVCKGSGW LEILGSGMVH PRVLEMAGYD 310 320 330 340 SSVVTGFAFG MGLERIAMLL YGIDDLRLFY DNDIRFLQMF

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB15132.1.
RefSeq	YP_360400.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3ABT4.
SMR	Q3ABT4. Positions 78-340.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3ABT4.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3727234.
GenomeReviews	Gene locus CHY_1571 in contig CP000141_GR.
KEGG	chy:CHY_1571.
NMPDR	fig|246194.3.peg.1554.
PATRIC	21276267. VBICarHyd26463_1505.
TIGR	CHY_1571.
Phylogenomic databases
eggNOG	COG0016.
HOGENOM	HBG284353.
OMA	FRASYFP.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_1571-MONOMER.
Family and domain databases
HAMAP	MF_00281. Phe_tRNA_synth_alpha1. [Tree]
InterPro	IPR006195. aa-tRNA-synth_II. IPR004529. Phe-tRNA-synth_IIc_asu. IPR004188. Phe-tRNA_synth_II_N. IPR022911. Phe_tRNA_synth_alpha1_bac. IPR002319. Phenylalanyl-tRNA_Synthase. IPR010978. tRNA-bd_arm. [Graphical view]
KO	K01889.
Pfam	PF02912. Phe_tRNA-synt_N. 1 hit. PF01409. tRNA-synt_2d. 1 hit. [Graphical view]
SUPFAM	SSF46589. tRNA_binding_arm. 1 hit.
TIGRFAMs	TIGR00468. PheS. 1 hit.
PROSITE	PS50862. AA_TRNA_LIGASE_II. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

SYFA_CARHZ

Accession

Primary (citable) accession number: Q3ABT4

Entry history

Integrated into UniProtKB/Swiss-Prot:	April 4, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 43 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program