Q3AAE8 (MURB_CARHZ) Reviewed, UniProtKB/Swiss-Prot

Names and origin

Protein names

Recommended name:
UDP-N-acetylenolpyruvoylglucosamine reductase
EC=1.1.1.158

Alternative name(s):
UDP-N-acetylmuramate dehydrogenase

Gene names

Name:	murB
Ordered Locus Names:	CHY_2067

Organism

Carboxydothermus hydrogenoformans (strain Z-2901 / DSM 6008) [Complete proteome] [HAMAP]

Taxonomic identifier

246194 [NCBI]

Taxonomic lineage

Bacteria › Firmicutes › Clostridia › Thermoanaerobacterales › Thermoanaerobacteriaceae › Carboxydothermus

Protein attributes

Sequence length	302 AA.
Sequence status	Complete.
Protein existence	Inferred from homology

General annotation (Comments)

Function	Cell wall formation By similarity. HAMAP MF_00037
Catalytic activity	UDP-N-acetylmuramate + NADP+ = UDP-N-acetyl-3-O-(1-carboxyvinyl)-D-glucosamine + NADPH. HAMAP MF_00037
Cofactor	FAD By similarity. HAMAP MF_00037
Pathway	Cell wall biogenesis; peptidoglycan biosynthesis. HAMAP MF_00037
Subcellular location	Cytoplasm By similarity HAMAP MF_00037.
Sequence similarities	Belongs to the MurB family. Contains 1 FAD-binding PCMH-type domain.

Ontologies

Keywords
Biological process	Cell cycle Cell division Cell shape Cell wall biogenesis/degradation Peptidoglycan synthesis
Cellular component	Cytoplasm
Ligand	FAD Flavoprotein NADP
Molecular function	Oxidoreductase
Technical term	Complete proteome
Gene Ontology (GO)
Biological process	cell cycle Inferred from electronic annotation. Source: UniProtKB-KW cell division Inferred from electronic annotation. Source: UniProtKB-KW cellular cell wall organization Inferred from electronic annotation. Source: UniProtKB-KW peptidoglycan biosynthetic process Inferred from electronic annotation. Source: UniProtKB-KW regulation of cell shape Inferred from electronic annotation. Source: UniProtKB-KW
Cellular component	cytoplasm Inferred from electronic annotation. Source: UniProtKB-SubCell
Molecular function	UDP-N-acetylmuramate dehydrogenase activity Inferred from electronic annotation. Source: EC flavin adenine dinucleotide binding Inferred from electronic annotation. Source: InterPro
Complete GO annotation...

Sequence annotation (Features)

	Feature key	Position(s)	Length	Description	Graphical view	Feature identifier
Molecule processing
	Chain	1 – 302	302	UDP-N-acetylenolpyruvoylglucosamine reductase HAMAP MF_00037		PRO_0000224674
Regions
	Domain	31 – 213	183	FAD-binding PCMH-type
Sites
	Active site	176	1	By similarity
	Active site	226	1	Proton donor By similarity
	Active site	296	1	By similarity

Sequences

	Sequence		Length	Mass (Da)
	Q3AAE8 [UniParc]. Last modified November 22, 2005. Version 1. Checksum: C57F425B929F49FC	FASTA	302	33,328
	10 20 30 40 50 60 MDKAKLKEEL TKRISSPVLE NEPLAQHTTW KIGGPADFLI EPQSIEELSL VIRFLTENAV 70 80 90 100 110 120 NFRVIGNGSN ILVLDRGFRG VIIKTKKINK VEITAGGQVF AEAGVLLPAL AARALKVGLS 130 140 150 160 170 180 GLEELCAIPG SVGGAIRQNA GAHGKEIKDV LKRVWTINER GELKEFFANE CGFKYRSSRF 190 200 210 220 230 240 KEEKQWIVKA EFSLNPGDKK EILKKIREFR EKRLASQPLE FPNAGSVFKN PEGIPAWKLI 250 260 270 280 290 300 KEAGAQGLKK GGAMVSEKHA NFIINTGGAS AADVIYLINK IQELVWKKFS VKLLLEVEVL GE

References

[1]

"Life in hot carbon monoxide: the complete genome sequence of Carboxydothermus hydrogenoformans Z-2901." Wu M., Ren Q., Durkin A.S., Daugherty S.C., Brinkac L.M., Dodson R.J., Madupu R., Sullivan S.A., Kolonay J.F., Nelson W.C., Tallon L.J., Jones K.M., Ulrich L.E., Gonzalez J.M., Zhulin I.B., Robb F.T., Eisen J.A. PLoS Genet. 1:563-574(2005) [PubMed: 16311624] [Abstract] Cited for: NUCLEOTIDE SEQUENCE [LARGE SCALE GENOMIC DNA]. Strain: Z-2901 / DSM 6008.

Cross-references

Sequence databases
EMBL GenBank DDBJ	CP000141 Genomic DNA. Translation: ABB15062.1.
RefSeq	YP_360886.1. NC_007503.1.
3D structure databases
ProteinModelPortal	Q3AAE8.
ModBase	Search...
Protein-protein interaction databases
STRING	Q3AAE8.
Protocols and materials databases
StructuralBiologyKnowledgebase	Search...
Genome annotation databases
GeneID	3727163.
GenomeReviews	Gene locus CHY_2067 in contig CP000141_GR.
KEGG	chy:CHY_2067.
NMPDR	fig|246194.3.peg.1855.
PATRIC	21277215. VBICarHyd26463_1970.
TIGR	CHY_2067.
Phylogenomic databases
eggNOG	COG0812.
HOGENOM	HBG686573.
OMA	SKKHAGF.
ProtClustDB	PRK13905.
Enzyme and pathway databases
BioCyc	CHYD246194:CHY_2067-MONOMER.
Family and domain databases
HAMAP	MF_00037. MurB. [Tree]
InterPro	IPR016169. CO_DH_flavot_FAD-bd_sub2. IPR016166. FAD-bd_2. IPR016167. FAD-bd_2_sub1. IPR003170. MurB. IPR011601. MurB_C. IPR006094. Oxid_FAD_bind_N. [Graphical view]
Gene3D	G3DSA:3.30.465.10. CO_DH_flavoprot_FAD-bd_sub2. 1 hit. G3DSA:3.30.43.10. FAD-binding_2_sub1. 1 hit. G3DSA:3.90.78.10. MurB_C. 1 hit.
KO	K00075.
PANTHER	PTHR21071. MurB. 1 hit.
Pfam	PF01565. FAD_binding_4. 1 hit. PF02873. MurB_C. 1 hit. [Graphical view]
SUPFAM	SSF56176. FAD-binding_2. 1 hit. SSF56194. MurB_C. 1 hit.
TIGRFAMs	TIGR00179. MurB. 1 hit.
PROSITE	PS51387. FAD_PCMH. 1 hit. [Graphical view]
ProtoNet	Search...

Entry information

Entry name

MURB_CARHZ

Accession

Primary (citable) accession number: Q3AAE8

Entry history

Integrated into UniProtKB/Swiss-Prot:	March 7, 2006
Last sequence update:	November 22, 2005
Last modified:	January 25, 2012
This is version 46 of the entry and version 1 of the sequence. [Complete history]

Entry status

Reviewed (UniProtKB/Swiss-Prot)

Annotation program

Prokaryotic Protein Annotation Program